Potential secondary metabolite biosynthetic gene clusters and antibacterial activity of novel taxa Gandjariella

Abstract. Ningsih F, Sari DCAF, Yabe S, Yokota A, Sjamsuridzal W. 2020. Potential secondary metabolite biosynthetic gene clusters and antibacterial activity of novel taxa Gandjariella. Biodiversitas 21: 5674-5684. Microbial resistance to available antibiotics has gained increasing attention in recen...

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Autores principales:	Fitria Ningsih, Dhian Chitra Ayu Fitria Sari, Shuhei Yabe, Akira Yokota, Wellyzar Sjamsuridzal
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	novel actinobacteria taxa, secondary metabolites, biosynthetic gene clusters, antibacterial activity Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/23edf3ce76784944bf77ab44e5700aca
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spelling	oai:doaj.org-article:23edf3ce76784944bf77ab44e5700aca2021-11-22T00:52:01ZPotential secondary metabolite biosynthetic gene clusters and antibacterial activity of novel taxa Gandjariella1412-033X2085-472210.13057/biodiv/d211225https://doaj.org/article/23edf3ce76784944bf77ab44e5700aca2020-11-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6819https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Ningsih F, Sari DCAF, Yabe S, Yokota A, Sjamsuridzal W. 2020. Potential secondary metabolite biosynthetic gene clusters and antibacterial activity of novel taxa Gandjariella. Biodiversitas 21: 5674-5684. Microbial resistance to available antibiotics has gained increasing attention in recent years and led to the urgent search for active secondary metabolites from novel microbial taxa. This study aimed to assess putative secondary metabolite biosynthetic gene clusters (BGCs) in the genome of a novel thermophilic Actinobacteria type strain Gandjariella thermophila SL3-2-4T and screen for its antibacterial activity. Four other related novel candidate Actinobacteria strains, isolated from forest soil in the Cisolok geothermal area (West Java, Indonesia), were also screened for antibacterial activity in various media solidified with gellan gum. The genome of the SL3-2-4T strain contained 21 antiSMASH-identified secondary metabolite regions harboring BGCs. These BGCs were for polyketide synthase, non-ribosomal peptide synthase, and ribosomally synthesized and post-translationally modified peptide family clusters. Three BGC regions displayed 50-100% similarity with known secondary metabolites. Thirteen and five regions displayed low (4-35%) and no similarity with known BGCs for secondary metabolites, respectively. Strains SL3-2-4T and SL3-2-7 on MM 2 medium solidified with gellan gum at 45 °C for 14 days demonstrated inhibitory activity against all Gram-positive, but not Gram-negative bacteria. Strain SL3-2-10 on ISP 3 gellan gum medium incubated for seven days only active against K. rhizophila NBRC 12078. Strains SL3-2-6 and SL3-2-9 did not exhibit any antibacterial activity against the tested bacterial strains on the three tested media. The results indicated that novel taxa have the potential for the discovery of active secondary metabolites.Fitria NingsihDhian Chitra Ayu Fitria SariShuhei YabeAkira YokotaWellyzar SjamsuridzalMBI & UNS Soloarticlenovel actinobacteria taxa, secondary metabolites, biosynthetic gene clusters, antibacterial activityBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	novel actinobacteria taxa, secondary metabolites, biosynthetic gene clusters, antibacterial activity Biology (General) QH301-705.5
spellingShingle	novel actinobacteria taxa, secondary metabolites, biosynthetic gene clusters, antibacterial activity Biology (General) QH301-705.5 Fitria Ningsih Dhian Chitra Ayu Fitria Sari Shuhei Yabe Akira Yokota Wellyzar Sjamsuridzal Potential secondary metabolite biosynthetic gene clusters and antibacterial activity of novel taxa Gandjariella
description	Abstract. Ningsih F, Sari DCAF, Yabe S, Yokota A, Sjamsuridzal W. 2020. Potential secondary metabolite biosynthetic gene clusters and antibacterial activity of novel taxa Gandjariella. Biodiversitas 21: 5674-5684. Microbial resistance to available antibiotics has gained increasing attention in recent years and led to the urgent search for active secondary metabolites from novel microbial taxa. This study aimed to assess putative secondary metabolite biosynthetic gene clusters (BGCs) in the genome of a novel thermophilic Actinobacteria type strain Gandjariella thermophila SL3-2-4T and screen for its antibacterial activity. Four other related novel candidate Actinobacteria strains, isolated from forest soil in the Cisolok geothermal area (West Java, Indonesia), were also screened for antibacterial activity in various media solidified with gellan gum. The genome of the SL3-2-4T strain contained 21 antiSMASH-identified secondary metabolite regions harboring BGCs. These BGCs were for polyketide synthase, non-ribosomal peptide synthase, and ribosomally synthesized and post-translationally modified peptide family clusters. Three BGC regions displayed 50-100% similarity with known secondary metabolites. Thirteen and five regions displayed low (4-35%) and no similarity with known BGCs for secondary metabolites, respectively. Strains SL3-2-4T and SL3-2-7 on MM 2 medium solidified with gellan gum at 45 °C for 14 days demonstrated inhibitory activity against all Gram-positive, but not Gram-negative bacteria. Strain SL3-2-10 on ISP 3 gellan gum medium incubated for seven days only active against K. rhizophila NBRC 12078. Strains SL3-2-6 and SL3-2-9 did not exhibit any antibacterial activity against the tested bacterial strains on the three tested media. The results indicated that novel taxa have the potential for the discovery of active secondary metabolites.
format	article
author	Fitria Ningsih Dhian Chitra Ayu Fitria Sari Shuhei Yabe Akira Yokota Wellyzar Sjamsuridzal
author_facet	Fitria Ningsih Dhian Chitra Ayu Fitria Sari Shuhei Yabe Akira Yokota Wellyzar Sjamsuridzal
author_sort	Fitria Ningsih
title	Potential secondary metabolite biosynthetic gene clusters and antibacterial activity of novel taxa Gandjariella
title_short	Potential secondary metabolite biosynthetic gene clusters and antibacterial activity of novel taxa Gandjariella
title_full	Potential secondary metabolite biosynthetic gene clusters and antibacterial activity of novel taxa Gandjariella
title_fullStr	Potential secondary metabolite biosynthetic gene clusters and antibacterial activity of novel taxa Gandjariella
title_full_unstemmed	Potential secondary metabolite biosynthetic gene clusters and antibacterial activity of novel taxa Gandjariella
title_sort	potential secondary metabolite biosynthetic gene clusters and antibacterial activity of novel taxa gandjariella
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/23edf3ce76784944bf77ab44e5700aca
work_keys_str_mv	AT fitrianingsih potentialsecondarymetabolitebiosyntheticgeneclustersandantibacterialactivityofnoveltaxagandjariella AT dhianchitraayufitriasari potentialsecondarymetabolitebiosyntheticgeneclustersandantibacterialactivityofnoveltaxagandjariella AT shuheiyabe potentialsecondarymetabolitebiosyntheticgeneclustersandantibacterialactivityofnoveltaxagandjariella AT akirayokota potentialsecondarymetabolitebiosyntheticgeneclustersandantibacterialactivityofnoveltaxagandjariella AT wellyzarsjamsuridzal potentialsecondarymetabolitebiosyntheticgeneclustersandantibacterialactivityofnoveltaxagandjariella
_version_	1718418446918615040

Potential secondary metabolite biosynthetic gene clusters and antibacterial activity of novel taxa Gandjariella

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