Structural and functional analysis of the role of the chaperonin CCT in mTOR complex assembly

β-propeller domains are an important class of folding substrates for the eukaryotic cytosolic chaperonin CTT. Here the authors find that CTT contributes to the folding and assembly of two β-propeller proteins from mTOR complexes, mLST8 and Raptor, and determine the 4.0 Å cryoEM structure of a human...

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Autores principales:	Jorge Cuéllar, W. Grant Ludlam, Nicole C. Tensmeyer, Takuma Aoba, Madhura Dhavale, César Santiago, M. Teresa Bueno-Carrasco, Michael J. Mann, Rebecca L. Plimpton, Aman Makaju, Sarah Franklin, Barry M. Willardson, José M. Valpuesta
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/23eeb432bf314670b0ab58f513d2d7e9
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spelling	oai:doaj.org-article:23eeb432bf314670b0ab58f513d2d7e92021-12-02T16:57:11ZStructural and functional analysis of the role of the chaperonin CCT in mTOR complex assembly10.1038/s41467-019-10781-12041-1723https://doaj.org/article/23eeb432bf314670b0ab58f513d2d7e92019-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10781-1https://doaj.org/toc/2041-1723β-propeller domains are an important class of folding substrates for the eukaryotic cytosolic chaperonin CTT. Here the authors find that CTT contributes to the folding and assembly of two β-propeller proteins from mTOR complexes, mLST8 and Raptor, and determine the 4.0 Å cryoEM structure of a human mLST8-CCT intermediate that shows mLST8 in a near-native state.Jorge CuéllarW. Grant LudlamNicole C. TensmeyerTakuma AobaMadhura DhavaleCésar SantiagoM. Teresa Bueno-CarrascoMichael J. MannRebecca L. PlimptonAman MakajuSarah FranklinBarry M. WillardsonJosé M. ValpuestaNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jorge Cuéllar W. Grant Ludlam Nicole C. Tensmeyer Takuma Aoba Madhura Dhavale César Santiago M. Teresa Bueno-Carrasco Michael J. Mann Rebecca L. Plimpton Aman Makaju Sarah Franklin Barry M. Willardson José M. Valpuesta Structural and functional analysis of the role of the chaperonin CCT in mTOR complex assembly
description	β-propeller domains are an important class of folding substrates for the eukaryotic cytosolic chaperonin CTT. Here the authors find that CTT contributes to the folding and assembly of two β-propeller proteins from mTOR complexes, mLST8 and Raptor, and determine the 4.0 Å cryoEM structure of a human mLST8-CCT intermediate that shows mLST8 in a near-native state.
format	article
author	Jorge Cuéllar W. Grant Ludlam Nicole C. Tensmeyer Takuma Aoba Madhura Dhavale César Santiago M. Teresa Bueno-Carrasco Michael J. Mann Rebecca L. Plimpton Aman Makaju Sarah Franklin Barry M. Willardson José M. Valpuesta
author_facet	Jorge Cuéllar W. Grant Ludlam Nicole C. Tensmeyer Takuma Aoba Madhura Dhavale César Santiago M. Teresa Bueno-Carrasco Michael J. Mann Rebecca L. Plimpton Aman Makaju Sarah Franklin Barry M. Willardson José M. Valpuesta
author_sort	Jorge Cuéllar
title	Structural and functional analysis of the role of the chaperonin CCT in mTOR complex assembly
title_short	Structural and functional analysis of the role of the chaperonin CCT in mTOR complex assembly
title_full	Structural and functional analysis of the role of the chaperonin CCT in mTOR complex assembly
title_fullStr	Structural and functional analysis of the role of the chaperonin CCT in mTOR complex assembly
title_full_unstemmed	Structural and functional analysis of the role of the chaperonin CCT in mTOR complex assembly
title_sort	structural and functional analysis of the role of the chaperonin cct in mtor complex assembly
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/23eeb432bf314670b0ab58f513d2d7e9
work_keys_str_mv	AT jorgecuellar structuralandfunctionalanalysisoftheroleofthechaperonincctinmtorcomplexassembly AT wgrantludlam structuralandfunctionalanalysisoftheroleofthechaperonincctinmtorcomplexassembly AT nicolectensmeyer structuralandfunctionalanalysisoftheroleofthechaperonincctinmtorcomplexassembly AT takumaaoba structuralandfunctionalanalysisoftheroleofthechaperonincctinmtorcomplexassembly AT madhuradhavale structuralandfunctionalanalysisoftheroleofthechaperonincctinmtorcomplexassembly AT cesarsantiago structuralandfunctionalanalysisoftheroleofthechaperonincctinmtorcomplexassembly AT mteresabuenocarrasco structuralandfunctionalanalysisoftheroleofthechaperonincctinmtorcomplexassembly AT michaeljmann structuralandfunctionalanalysisoftheroleofthechaperonincctinmtorcomplexassembly AT rebeccalplimpton structuralandfunctionalanalysisoftheroleofthechaperonincctinmtorcomplexassembly AT amanmakaju structuralandfunctionalanalysisoftheroleofthechaperonincctinmtorcomplexassembly AT sarahfranklin structuralandfunctionalanalysisoftheroleofthechaperonincctinmtorcomplexassembly AT barrymwillardson structuralandfunctionalanalysisoftheroleofthechaperonincctinmtorcomplexassembly AT josemvalpuesta structuralandfunctionalanalysisoftheroleofthechaperonincctinmtorcomplexassembly
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Structural and functional analysis of the role of the chaperonin CCT in mTOR complex assembly

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