Early growth response gene-2 (Egr-2) regulates the development of B and T cells.

<h4>Background</h4>Understanding of how transcription factors are involved in lymphocyte development still remains a challenge. It has been shown that Egr-2 deficiency results in impaired NKT cell development and defective positive selection of T cells. Here we investigated the developme...

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Autores principales:	Suling Li, Alistair L J Symonds, Bo Zhu, Mengya Liu, Meera V Raymond, Tizong Miao, Ping Wang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/23f18569708346089680a0fc4a3bb7a4
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spelling	oai:doaj.org-article:23f18569708346089680a0fc4a3bb7a42021-11-18T06:55:43ZEarly growth response gene-2 (Egr-2) regulates the development of B and T cells.1932-620310.1371/journal.pone.0018498https://doaj.org/article/23f18569708346089680a0fc4a3bb7a42011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21533228/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Understanding of how transcription factors are involved in lymphocyte development still remains a challenge. It has been shown that Egr-2 deficiency results in impaired NKT cell development and defective positive selection of T cells. Here we investigated the development of T, B and NKT cells in Egr-2 transgenic mice and the roles in the regulation of distinct stages of B and T cell development.<h4>Methods and findings</h4>The expression of Egr1, 2 and 3 were analysed at different stages of T and B cell development by RT-PCT and results showed that the expression was strictly regulated at different stages. Forced expression of Egr-2 in CD2(+) lymphocytes resulted in a severe reduction of CD4(+)CD8(+) (DP) cells in thymus and pro-B cells in bone marrow, which was associated with reduced expression of Notch1 in ISP thymocytes and Pax5 in pro-B cells, suggesting that retraction of Egr-2 at the ISP and pro-B cell stages is important for the activation of lineage differentiation programs. In contrast to reduction of DP and pro-B cells, Egr-2 enhanced the maturation of DP cells into single positive (SP) T and NKT cells in thymus, and immature B cells into mature B cells in bone marrow.<h4>Conclusions</h4>Our results demonstrate that Egr-2 expressed in restricted stages of lymphocyte development plays a dynamic, but similar role for the development of T, NKT and B cells.Suling LiAlistair L J SymondsBo ZhuMengya LiuMeera V RaymondTizong MiaoPing WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 4, p e18498 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Suling Li Alistair L J Symonds Bo Zhu Mengya Liu Meera V Raymond Tizong Miao Ping Wang Early growth response gene-2 (Egr-2) regulates the development of B and T cells.
description	<h4>Background</h4>Understanding of how transcription factors are involved in lymphocyte development still remains a challenge. It has been shown that Egr-2 deficiency results in impaired NKT cell development and defective positive selection of T cells. Here we investigated the development of T, B and NKT cells in Egr-2 transgenic mice and the roles in the regulation of distinct stages of B and T cell development.<h4>Methods and findings</h4>The expression of Egr1, 2 and 3 were analysed at different stages of T and B cell development by RT-PCT and results showed that the expression was strictly regulated at different stages. Forced expression of Egr-2 in CD2(+) lymphocytes resulted in a severe reduction of CD4(+)CD8(+) (DP) cells in thymus and pro-B cells in bone marrow, which was associated with reduced expression of Notch1 in ISP thymocytes and Pax5 in pro-B cells, suggesting that retraction of Egr-2 at the ISP and pro-B cell stages is important for the activation of lineage differentiation programs. In contrast to reduction of DP and pro-B cells, Egr-2 enhanced the maturation of DP cells into single positive (SP) T and NKT cells in thymus, and immature B cells into mature B cells in bone marrow.<h4>Conclusions</h4>Our results demonstrate that Egr-2 expressed in restricted stages of lymphocyte development plays a dynamic, but similar role for the development of T, NKT and B cells.
format	article
author	Suling Li Alistair L J Symonds Bo Zhu Mengya Liu Meera V Raymond Tizong Miao Ping Wang
author_facet	Suling Li Alistair L J Symonds Bo Zhu Mengya Liu Meera V Raymond Tizong Miao Ping Wang
author_sort	Suling Li
title	Early growth response gene-2 (Egr-2) regulates the development of B and T cells.
title_short	Early growth response gene-2 (Egr-2) regulates the development of B and T cells.
title_full	Early growth response gene-2 (Egr-2) regulates the development of B and T cells.
title_fullStr	Early growth response gene-2 (Egr-2) regulates the development of B and T cells.
title_full_unstemmed	Early growth response gene-2 (Egr-2) regulates the development of B and T cells.
title_sort	early growth response gene-2 (egr-2) regulates the development of b and t cells.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/23f18569708346089680a0fc4a3bb7a4
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Early growth response gene-2 (Egr-2) regulates the development of B and T cells.

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