Pathogenic Mechanisms of Preeclampsia with Severe Features Implied by the Plasma Exosomal miRNA Profile

Preeclampsia is a complication of pregnancy characterised by high blood pressure and organ damage after 20 gestational weeks. It is associated with high maternal and fetal morbidity and mortality; however, at present, there is no effective prevention or treatment for this condition. Previous studies...

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Autores principales:	Zhirui Chen, Wen Zhang, Mengying Wu, Haixia Huang, Li Zou, Qingqing Luo
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	exosomes mirnas profile pre-eclampsia gene ontology kegg endothelial cell dysfunction Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/24093d4ca6dc49a4979145698cd858a7
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spelling	oai:doaj.org-article:24093d4ca6dc49a4979145698cd858a72021-11-04T15:51:54ZPathogenic Mechanisms of Preeclampsia with Severe Features Implied by the Plasma Exosomal miRNA Profile2165-59792165-598710.1080/21655979.2021.1993717https://doaj.org/article/24093d4ca6dc49a4979145698cd858a72021-10-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1993717https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Preeclampsia is a complication of pregnancy characterised by high blood pressure and organ damage after 20 gestational weeks. It is associated with high maternal and fetal morbidity and mortality; however, at present, there is no effective prevention or treatment for this condition. Previous studies have revealed that plasma exosomal miRNAs from pregnant women with preeclampsia could serve as biomarkers of pathogenic factors. However, the roles of plasma exosomal miRNAs in preeclampsia with severe features (sPE), which is associated with poorer pregnancy outcomes, remain unknown. Thus, the aims of this study were to characterise plasma exosomal miRNAs in sPE and explore the related pathogenic mechanisms using bioinformatic analysis. Plasma exosomes were isolated using a mirVana RNA isolation kit. The exosomal miRNAs were detected using high-throughput sequencing and the miRNAs related to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways and gene ontology (GO) terms were analysed using the clusterProfiler package of R. Fifteen miRNAs exhibited increased expression and fourteen miRNAs exhibited reduced expression in plasma exosomes from women with sPE as compared to normal pregnant women. Further, gene set enrichment analysis revealed that the differentially expressed plasma exosomal miRNAs were related to the stress response and cell junction regulation, among others. In summary, this study is the first to identify the differentially expressed plasma exosomal miRNAs in sPE. These findings highlight promising pathogenesis mechanisms underlying preeclampsia.Zhirui ChenWen ZhangMengying WuHaixia HuangLi ZouQingqing LuoTaylor & Francis Grouparticleexosomesmirnas profilepre-eclampsiagene ontologykeggendothelial cell dysfunctionBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	exosomes mirnas profile pre-eclampsia gene ontology kegg endothelial cell dysfunction Biotechnology TP248.13-248.65
spellingShingle	exosomes mirnas profile pre-eclampsia gene ontology kegg endothelial cell dysfunction Biotechnology TP248.13-248.65 Zhirui Chen Wen Zhang Mengying Wu Haixia Huang Li Zou Qingqing Luo Pathogenic Mechanisms of Preeclampsia with Severe Features Implied by the Plasma Exosomal miRNA Profile
description	Preeclampsia is a complication of pregnancy characterised by high blood pressure and organ damage after 20 gestational weeks. It is associated with high maternal and fetal morbidity and mortality; however, at present, there is no effective prevention or treatment for this condition. Previous studies have revealed that plasma exosomal miRNAs from pregnant women with preeclampsia could serve as biomarkers of pathogenic factors. However, the roles of plasma exosomal miRNAs in preeclampsia with severe features (sPE), which is associated with poorer pregnancy outcomes, remain unknown. Thus, the aims of this study were to characterise plasma exosomal miRNAs in sPE and explore the related pathogenic mechanisms using bioinformatic analysis. Plasma exosomes were isolated using a mirVana RNA isolation kit. The exosomal miRNAs were detected using high-throughput sequencing and the miRNAs related to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways and gene ontology (GO) terms were analysed using the clusterProfiler package of R. Fifteen miRNAs exhibited increased expression and fourteen miRNAs exhibited reduced expression in plasma exosomes from women with sPE as compared to normal pregnant women. Further, gene set enrichment analysis revealed that the differentially expressed plasma exosomal miRNAs were related to the stress response and cell junction regulation, among others. In summary, this study is the first to identify the differentially expressed plasma exosomal miRNAs in sPE. These findings highlight promising pathogenesis mechanisms underlying preeclampsia.
format	article
author	Zhirui Chen Wen Zhang Mengying Wu Haixia Huang Li Zou Qingqing Luo
author_facet	Zhirui Chen Wen Zhang Mengying Wu Haixia Huang Li Zou Qingqing Luo
author_sort	Zhirui Chen
title	Pathogenic Mechanisms of Preeclampsia with Severe Features Implied by the Plasma Exosomal miRNA Profile
title_short	Pathogenic Mechanisms of Preeclampsia with Severe Features Implied by the Plasma Exosomal miRNA Profile
title_full	Pathogenic Mechanisms of Preeclampsia with Severe Features Implied by the Plasma Exosomal miRNA Profile
title_fullStr	Pathogenic Mechanisms of Preeclampsia with Severe Features Implied by the Plasma Exosomal miRNA Profile
title_full_unstemmed	Pathogenic Mechanisms of Preeclampsia with Severe Features Implied by the Plasma Exosomal miRNA Profile
title_sort	pathogenic mechanisms of preeclampsia with severe features implied by the plasma exosomal mirna profile
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/24093d4ca6dc49a4979145698cd858a7
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Pathogenic Mechanisms of Preeclampsia with Severe Features Implied by the Plasma Exosomal miRNA Profile

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