All-printed highly sensitive 2D MoS2 based multi-reagent immunosensor for smartphone based point-of-care diagnosis

Abstract Immunosensors are used to detect the presence of certain bio-reagents mostly targeted at the diagnosis of a condition or a disease. Here, a general purpose electrical immunosensor has been fabricated for the quantitative detection of multiple bio-reagents through the formation of an antibod...

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Autores principales:	Memoon Sajid, Ahmed Osman, Ghayas Uddin Siddiqui, Hyun Bum Kim, Soo Wan Kim, Jeong Bum Ko, Yoon Kyu Lim, Kyung Hyun Choi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2411ce09b97540ba95644b80691a6d17
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spelling	oai:doaj.org-article:2411ce09b97540ba95644b80691a6d172021-12-02T16:07:46ZAll-printed highly sensitive 2D MoS2 based multi-reagent immunosensor for smartphone based point-of-care diagnosis10.1038/s41598-017-06265-12045-2322https://doaj.org/article/2411ce09b97540ba95644b80691a6d172017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06265-1https://doaj.org/toc/2045-2322Abstract Immunosensors are used to detect the presence of certain bio-reagents mostly targeted at the diagnosis of a condition or a disease. Here, a general purpose electrical immunosensor has been fabricated for the quantitative detection of multiple bio-reagents through the formation of an antibody-antigen pair. The sensors were fabricated using all printing approaches. 2D transition metal dichalcogenide (TMDC) MoS2 thin film was deposited using Electrohydrodynamic atomization (EHDA) on top of an interdigitated transducer (IDT) electrode fabricated by reverse offset printing. The sensors were then treated with three different types of antibodies that were immobilized by physisorption into the highly porous multi-layered structure of MoS2 active layer. BSA was used as blocking agent to prevent non-specific absorption (NSA). The sensors were then employed for the targeted detection of the specific antigens including prostate specific antigen (PSA), mouse immunoglobulin-G (IgG), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). IgG was then selected to test the sensors for point of care (POC) diagnosis through a specially designed electronic readout system for sensors and interfacing it with a smartphone using Bluetooth connection. The sensors showed promising performance in terms of stability, specificity, repeatability, sensitivity, limit of detection (LoD), and range of detection (RoD).Memoon SajidAhmed OsmanGhayas Uddin SiddiquiHyun Bum KimSoo Wan KimJeong Bum KoYoon Kyu LimKyung Hyun ChoiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Memoon Sajid Ahmed Osman Ghayas Uddin Siddiqui Hyun Bum Kim Soo Wan Kim Jeong Bum Ko Yoon Kyu Lim Kyung Hyun Choi All-printed highly sensitive 2D MoS2 based multi-reagent immunosensor for smartphone based point-of-care diagnosis
description	Abstract Immunosensors are used to detect the presence of certain bio-reagents mostly targeted at the diagnosis of a condition or a disease. Here, a general purpose electrical immunosensor has been fabricated for the quantitative detection of multiple bio-reagents through the formation of an antibody-antigen pair. The sensors were fabricated using all printing approaches. 2D transition metal dichalcogenide (TMDC) MoS2 thin film was deposited using Electrohydrodynamic atomization (EHDA) on top of an interdigitated transducer (IDT) electrode fabricated by reverse offset printing. The sensors were then treated with three different types of antibodies that were immobilized by physisorption into the highly porous multi-layered structure of MoS2 active layer. BSA was used as blocking agent to prevent non-specific absorption (NSA). The sensors were then employed for the targeted detection of the specific antigens including prostate specific antigen (PSA), mouse immunoglobulin-G (IgG), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). IgG was then selected to test the sensors for point of care (POC) diagnosis through a specially designed electronic readout system for sensors and interfacing it with a smartphone using Bluetooth connection. The sensors showed promising performance in terms of stability, specificity, repeatability, sensitivity, limit of detection (LoD), and range of detection (RoD).
format	article
author	Memoon Sajid Ahmed Osman Ghayas Uddin Siddiqui Hyun Bum Kim Soo Wan Kim Jeong Bum Ko Yoon Kyu Lim Kyung Hyun Choi
author_facet	Memoon Sajid Ahmed Osman Ghayas Uddin Siddiqui Hyun Bum Kim Soo Wan Kim Jeong Bum Ko Yoon Kyu Lim Kyung Hyun Choi
author_sort	Memoon Sajid
title	All-printed highly sensitive 2D MoS2 based multi-reagent immunosensor for smartphone based point-of-care diagnosis
title_short	All-printed highly sensitive 2D MoS2 based multi-reagent immunosensor for smartphone based point-of-care diagnosis
title_full	All-printed highly sensitive 2D MoS2 based multi-reagent immunosensor for smartphone based point-of-care diagnosis
title_fullStr	All-printed highly sensitive 2D MoS2 based multi-reagent immunosensor for smartphone based point-of-care diagnosis
title_full_unstemmed	All-printed highly sensitive 2D MoS2 based multi-reagent immunosensor for smartphone based point-of-care diagnosis
title_sort	all-printed highly sensitive 2d mos2 based multi-reagent immunosensor for smartphone based point-of-care diagnosis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2411ce09b97540ba95644b80691a6d17
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_version_	1718384704422412288

All-printed highly sensitive 2D MoS2 based multi-reagent immunosensor for smartphone based point-of-care diagnosis

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