Antibiotic resistance plasmid composition and architecture in Escherichia coli isolates from meat

Abstract Resistance plasmids play a crucial role in the transfer of antimicrobial resistance from the veterinary sector to human healthcare. In this study plasmids from foodborne Escherichia coli isolates with a known (ES)BL or tetracycline resistance were sequenced entirely with short- and long-rea...

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Autores principales:	Tania S. Darphorn, Keshia Bel, Belinda B. Koenders-van Sint Anneland, Stanley Brul, Benno H. Ter Kuile
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2412464a07e444d7898d0487f7198021
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spelling	oai:doaj.org-article:2412464a07e444d7898d0487f71980212021-12-02T10:49:11ZAntibiotic resistance plasmid composition and architecture in Escherichia coli isolates from meat10.1038/s41598-021-81683-w2045-2322https://doaj.org/article/2412464a07e444d7898d0487f71980212021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81683-whttps://doaj.org/toc/2045-2322Abstract Resistance plasmids play a crucial role in the transfer of antimicrobial resistance from the veterinary sector to human healthcare. In this study plasmids from foodborne Escherichia coli isolates with a known (ES)BL or tetracycline resistance were sequenced entirely with short- and long-read technologies to obtain insight into their composition and to identify driving factors for spreading. Resistant foodborne E. coli isolates often contained several plasmids coding for resistance to various antimicrobials. Most plasmids were large and contained multiple resistance genes in addition to the selected resistance gene. The majority of plasmids belonged to the IncI, IncF and IncX incompatibility groups. Conserved and variable regions could be distinguished in each of the plasmid groups. Clusters containing resistance genes were located in the variable regions. Tetracycline and (extended spectrum) beta-lactamase resistance genes were each situated in separate clusters, but sulphonamide, macrolide and aminoglycoside formed one cluster and lincosamide and aminoglycoside another. In most plasmids, addiction systems were found to maintain presence in the cell.Tania S. DarphornKeshia BelBelinda B. Koenders-van Sint AnnelandStanley BrulBenno H. Ter KuileNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tania S. Darphorn Keshia Bel Belinda B. Koenders-van Sint Anneland Stanley Brul Benno H. Ter Kuile Antibiotic resistance plasmid composition and architecture in Escherichia coli isolates from meat
description	Abstract Resistance plasmids play a crucial role in the transfer of antimicrobial resistance from the veterinary sector to human healthcare. In this study plasmids from foodborne Escherichia coli isolates with a known (ES)BL or tetracycline resistance were sequenced entirely with short- and long-read technologies to obtain insight into their composition and to identify driving factors for spreading. Resistant foodborne E. coli isolates often contained several plasmids coding for resistance to various antimicrobials. Most plasmids were large and contained multiple resistance genes in addition to the selected resistance gene. The majority of plasmids belonged to the IncI, IncF and IncX incompatibility groups. Conserved and variable regions could be distinguished in each of the plasmid groups. Clusters containing resistance genes were located in the variable regions. Tetracycline and (extended spectrum) beta-lactamase resistance genes were each situated in separate clusters, but sulphonamide, macrolide and aminoglycoside formed one cluster and lincosamide and aminoglycoside another. In most plasmids, addiction systems were found to maintain presence in the cell.
format	article
author	Tania S. Darphorn Keshia Bel Belinda B. Koenders-van Sint Anneland Stanley Brul Benno H. Ter Kuile
author_facet	Tania S. Darphorn Keshia Bel Belinda B. Koenders-van Sint Anneland Stanley Brul Benno H. Ter Kuile
author_sort	Tania S. Darphorn
title	Antibiotic resistance plasmid composition and architecture in Escherichia coli isolates from meat
title_short	Antibiotic resistance plasmid composition and architecture in Escherichia coli isolates from meat
title_full	Antibiotic resistance plasmid composition and architecture in Escherichia coli isolates from meat
title_fullStr	Antibiotic resistance plasmid composition and architecture in Escherichia coli isolates from meat
title_full_unstemmed	Antibiotic resistance plasmid composition and architecture in Escherichia coli isolates from meat
title_sort	antibiotic resistance plasmid composition and architecture in escherichia coli isolates from meat
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2412464a07e444d7898d0487f7198021
work_keys_str_mv	AT taniasdarphorn antibioticresistanceplasmidcompositionandarchitectureinescherichiacoliisolatesfrommeat AT keshiabel antibioticresistanceplasmidcompositionandarchitectureinescherichiacoliisolatesfrommeat AT belindabkoendersvansintanneland antibioticresistanceplasmidcompositionandarchitectureinescherichiacoliisolatesfrommeat AT stanleybrul antibioticresistanceplasmidcompositionandarchitectureinescherichiacoliisolatesfrommeat AT bennohterkuile antibioticresistanceplasmidcompositionandarchitectureinescherichiacoliisolatesfrommeat
_version_	1718396576193314816

Antibiotic resistance plasmid composition and architecture in Escherichia coli isolates from meat

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