PECULIARITIES OF ALLOGENIC INTERACTIONS IN THE SHORT-TERM CULTURE OF LYMPHOCYTES OF SPOUSES WHO HAVE CHILDREN WITH CONGENITAL HEART DISEASES OR EARLY REPRODUCTIVE LOSSES

We have studied HLA allogeneic interactions in short-term cultures of lymphocytes from the parents having children with congenital heart defects (CHD), or subject to early reproductive losses. Twentyone married couples (CHD as the main group) who had children with sporadic CHD (interventricular sept...

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Autores principales:	A. V. Shabaldin, S. A. Shmulevich, G. N. Chistyakova, I. I. Remizova, E. B. Lukoyanycheva, S. V. Gorshkova, E. V. Shabaldina
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2019
Materias:	mixed lymphocyte culture reproductive loss congenital heart disease hla-dr Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/24189bf27b7f4e7da947e24cd403a177
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spelling	oai:doaj.org-article:24189bf27b7f4e7da947e24cd403a1772021-11-18T08:03:48ZPECULIARITIES OF ALLOGENIC INTERACTIONS IN THE SHORT-TERM CULTURE OF LYMPHOCYTES OF SPOUSES WHO HAVE CHILDREN WITH CONGENITAL HEART DISEASES OR EARLY REPRODUCTIVE LOSSES1563-06252313-741X10.15789/1563-0625-2019-2-279-292https://doaj.org/article/24189bf27b7f4e7da947e24cd403a1772019-05-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1511https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XWe have studied HLA allogeneic interactions in short-term cultures of lymphocytes from the parents having children with congenital heart defects (CHD), or subject to early reproductive losses. Twentyone married couples (CHD as the main group) who had children with sporadic CHD (interventricular septal defect) without chromosomal diseases were observed. Fifty married couples (a comparison group) had two or more reproductive losses in early gestation (up to 9 weeks), denoted as PNPs (miscarriages, missed abortions, habitual miscarriages. Forty-one families with three or more healthy children represented a control group. Immune response in cell cultures was evaluated by increasing expression of HLA-DR in a mixed culture, as compared to spontaneous lymphocyte cultures. Initial labeling of female and male lymphocytes with monoclonal antibodies to CD45 conjugated to different fluorescent dyes (PC-5 and PC-7) allowed us to evaluate the immune response of female lymphocytes to males and vice versa. The suppressor effect of autologous female serum upon the mixed culture of the lymphocytes of the spouses was also evaluated. Results of the present study showed a difference in HLA allogeneic interactions in the short-term culture of lymphocytes registered for spouses with reproductive losses and children with congenital heart defects. Reproductive losses were associated with a low blocking effect of female auto-serum upon allogeneic HLA interactions in the short-term culture of the lymphocytes of the spouses. Congenital heart defects were associated with high activity of female B-lymphocytes (CD3-/HL-DR+) in short-term mixed culture of lymphocytes from the spouses.A. V. ShabaldinS. A. ShmulevichG. N. ChistyakovaI. I. RemizovaE. B. LukoyanychevaS. V. GorshkovaE. V. ShabaldinaSPb RAACIarticlemixed lymphocyte culturereproductive losscongenital heart diseasehla-drImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 21, Iss 2, Pp 279-292 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	mixed lymphocyte culture reproductive loss congenital heart disease hla-dr Immunologic diseases. Allergy RC581-607
spellingShingle	mixed lymphocyte culture reproductive loss congenital heart disease hla-dr Immunologic diseases. Allergy RC581-607 A. V. Shabaldin S. A. Shmulevich G. N. Chistyakova I. I. Remizova E. B. Lukoyanycheva S. V. Gorshkova E. V. Shabaldina PECULIARITIES OF ALLOGENIC INTERACTIONS IN THE SHORT-TERM CULTURE OF LYMPHOCYTES OF SPOUSES WHO HAVE CHILDREN WITH CONGENITAL HEART DISEASES OR EARLY REPRODUCTIVE LOSSES
description	We have studied HLA allogeneic interactions in short-term cultures of lymphocytes from the parents having children with congenital heart defects (CHD), or subject to early reproductive losses. Twentyone married couples (CHD as the main group) who had children with sporadic CHD (interventricular septal defect) without chromosomal diseases were observed. Fifty married couples (a comparison group) had two or more reproductive losses in early gestation (up to 9 weeks), denoted as PNPs (miscarriages, missed abortions, habitual miscarriages. Forty-one families with three or more healthy children represented a control group. Immune response in cell cultures was evaluated by increasing expression of HLA-DR in a mixed culture, as compared to spontaneous lymphocyte cultures. Initial labeling of female and male lymphocytes with monoclonal antibodies to CD45 conjugated to different fluorescent dyes (PC-5 and PC-7) allowed us to evaluate the immune response of female lymphocytes to males and vice versa. The suppressor effect of autologous female serum upon the mixed culture of the lymphocytes of the spouses was also evaluated. Results of the present study showed a difference in HLA allogeneic interactions in the short-term culture of lymphocytes registered for spouses with reproductive losses and children with congenital heart defects. Reproductive losses were associated with a low blocking effect of female auto-serum upon allogeneic HLA interactions in the short-term culture of the lymphocytes of the spouses. Congenital heart defects were associated with high activity of female B-lymphocytes (CD3-/HL-DR+) in short-term mixed culture of lymphocytes from the spouses.
format	article
author	A. V. Shabaldin S. A. Shmulevich G. N. Chistyakova I. I. Remizova E. B. Lukoyanycheva S. V. Gorshkova E. V. Shabaldina
author_facet	A. V. Shabaldin S. A. Shmulevich G. N. Chistyakova I. I. Remizova E. B. Lukoyanycheva S. V. Gorshkova E. V. Shabaldina
author_sort	A. V. Shabaldin
title	PECULIARITIES OF ALLOGENIC INTERACTIONS IN THE SHORT-TERM CULTURE OF LYMPHOCYTES OF SPOUSES WHO HAVE CHILDREN WITH CONGENITAL HEART DISEASES OR EARLY REPRODUCTIVE LOSSES
title_short	PECULIARITIES OF ALLOGENIC INTERACTIONS IN THE SHORT-TERM CULTURE OF LYMPHOCYTES OF SPOUSES WHO HAVE CHILDREN WITH CONGENITAL HEART DISEASES OR EARLY REPRODUCTIVE LOSSES
title_full	PECULIARITIES OF ALLOGENIC INTERACTIONS IN THE SHORT-TERM CULTURE OF LYMPHOCYTES OF SPOUSES WHO HAVE CHILDREN WITH CONGENITAL HEART DISEASES OR EARLY REPRODUCTIVE LOSSES
title_fullStr	PECULIARITIES OF ALLOGENIC INTERACTIONS IN THE SHORT-TERM CULTURE OF LYMPHOCYTES OF SPOUSES WHO HAVE CHILDREN WITH CONGENITAL HEART DISEASES OR EARLY REPRODUCTIVE LOSSES
title_full_unstemmed	PECULIARITIES OF ALLOGENIC INTERACTIONS IN THE SHORT-TERM CULTURE OF LYMPHOCYTES OF SPOUSES WHO HAVE CHILDREN WITH CONGENITAL HEART DISEASES OR EARLY REPRODUCTIVE LOSSES
title_sort	peculiarities of allogenic interactions in the short-term culture of lymphocytes of spouses who have children with congenital heart diseases or early reproductive losses
publisher	SPb RAACI
publishDate	2019
url	https://doaj.org/article/24189bf27b7f4e7da947e24cd403a177
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PECULIARITIES OF ALLOGENIC INTERACTIONS IN THE SHORT-TERM CULTURE OF LYMPHOCYTES OF SPOUSES WHO HAVE CHILDREN WITH CONGENITAL HEART DISEASES OR EARLY REPRODUCTIVE LOSSES

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