Comparison of the adhesion of calcium oxalate monohydrate to HK-2 cells before and after repair using tea polysaccharides

Yao-Wang Zhao,1 Da Guo,2 Chuang-Ye Li,1 Jian-Ming Ouyang21Department of Urology, Hunan Children’s Hospital, Changsha 410007, People’s Republic of China; 2Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, People’s Republic of Chin...

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Autores principales:	Zhao YW, Guo D, Li CY, Ouyang JM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Tea polysaccharide kidney stone crystal adhesion molecular weight Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/241aacf3c53f4482b548b9066ff935e4
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spelling	oai:doaj.org-article:241aacf3c53f4482b548b9066ff935e42021-12-02T11:27:07ZComparison of the adhesion of calcium oxalate monohydrate to HK-2 cells before and after repair using tea polysaccharides1178-2013https://doaj.org/article/241aacf3c53f4482b548b9066ff935e42019-06-01T00:00:00Zhttps://www.dovepress.com/comparison-of-the-adhesion-of-calcium-oxalate-monohydrate-to-hk-2-cell-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yao-Wang Zhao,1 Da Guo,2 Chuang-Ye Li,1 Jian-Ming Ouyang21Department of Urology, Hunan Children’s Hospital, Changsha 410007, People’s Republic of China; 2Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, People’s Republic of ChinaBackground: Kidney stone formation is closely related to renal epithelial cell damage and the adhesion of calcium oxalate crystals to cells.Methods: In this research, the adhesion of human kidney proximal tubular epithelial cells (HK-2) to calcium oxalate monohydrate crystals with a size of approximately 100 nm was studied. In addition, the inhibition of crystal adhesion by four tea polysaccharides (TPS0, TPS1, TPS2, and TPS3) with the molecular weights of 10.88, 8.16, 4.82, and 2.31 kDa, respectively were compared.Results: When oxalic acid-damaged HK-2 cells were repaired, cell viability increased. By contrast, reactive oxygen species level, phosphatidylserine eversion, and osteopontin expression decreased, thus indicating that tea polysaccharides have a repairing effect on damaged HK-2 cells. Moreover, after repairing the damaged cells, the amount of adherent crystals was reduced. The repair effect of tea polysaccharides is closely related to molecular weight, and TPS2 with the moderate molecular weight displayed the best repair effect.Conclusion: These results suggest that tea polysaccharides, especially TPS2, may inhibit the formation and recurrence of calcium oxalate kidney stones.Keywords: tea polysaccharide, kidney stone, crystal adhesion, molecular weight  Zhao YWGuo DLi CYOuyang JMDove Medical PressarticleTea polysaccharidekidney stonecrystal adhesionmolecular weightMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 4277-4292 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Tea polysaccharide kidney stone crystal adhesion molecular weight Medicine (General) R5-920
spellingShingle	Tea polysaccharide kidney stone crystal adhesion molecular weight Medicine (General) R5-920 Zhao YW Guo D Li CY Ouyang JM Comparison of the adhesion of calcium oxalate monohydrate to HK-2 cells before and after repair using tea polysaccharides
description	Yao-Wang Zhao,1 Da Guo,2 Chuang-Ye Li,1 Jian-Ming Ouyang21Department of Urology, Hunan Children’s Hospital, Changsha 410007, People’s Republic of China; 2Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, People’s Republic of ChinaBackground: Kidney stone formation is closely related to renal epithelial cell damage and the adhesion of calcium oxalate crystals to cells.Methods: In this research, the adhesion of human kidney proximal tubular epithelial cells (HK-2) to calcium oxalate monohydrate crystals with a size of approximately 100 nm was studied. In addition, the inhibition of crystal adhesion by four tea polysaccharides (TPS0, TPS1, TPS2, and TPS3) with the molecular weights of 10.88, 8.16, 4.82, and 2.31 kDa, respectively were compared.Results: When oxalic acid-damaged HK-2 cells were repaired, cell viability increased. By contrast, reactive oxygen species level, phosphatidylserine eversion, and osteopontin expression decreased, thus indicating that tea polysaccharides have a repairing effect on damaged HK-2 cells. Moreover, after repairing the damaged cells, the amount of adherent crystals was reduced. The repair effect of tea polysaccharides is closely related to molecular weight, and TPS2 with the moderate molecular weight displayed the best repair effect.Conclusion: These results suggest that tea polysaccharides, especially TPS2, may inhibit the formation and recurrence of calcium oxalate kidney stones.Keywords: tea polysaccharide, kidney stone, crystal adhesion, molecular weight
format	article
author	Zhao YW Guo D Li CY Ouyang JM
author_facet	Zhao YW Guo D Li CY Ouyang JM
author_sort	Zhao YW
title	Comparison of the adhesion of calcium oxalate monohydrate to HK-2 cells before and after repair using tea polysaccharides
title_short	Comparison of the adhesion of calcium oxalate monohydrate to HK-2 cells before and after repair using tea polysaccharides
title_full	Comparison of the adhesion of calcium oxalate monohydrate to HK-2 cells before and after repair using tea polysaccharides
title_fullStr	Comparison of the adhesion of calcium oxalate monohydrate to HK-2 cells before and after repair using tea polysaccharides
title_full_unstemmed	Comparison of the adhesion of calcium oxalate monohydrate to HK-2 cells before and after repair using tea polysaccharides
title_sort	comparison of the adhesion of calcium oxalate monohydrate to hk-2 cells before and after repair using tea polysaccharides
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/241aacf3c53f4482b548b9066ff935e4
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Comparison of the adhesion of calcium oxalate monohydrate to HK-2 cells before and after repair using tea polysaccharides

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