Sperm associated antigen 9 plays an important role in bladder transitional cell carcinoma.

<h4>Background</h4>Majority of bladder cancer deaths are caused due to transitional cell carcinoma (TCC) which is the most prevalent and chemoresistant malignancy of urinary bladder. Therefore, we analyzed the role of Sperm associated antigen 9 (SPAG9) in bladder TCC.<h4>Methodolog...

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Autores principales:	Deepika Kanojia, Manoj Garg, Shikha Saini, Sumit Agarwal, Deepak Parashar, Nirmala Jagadish, Amlesh Seth, Amar Bhatnagar, Anju Gupta, Rajive Kumar, Nirmal Kumar Lohiya, Anil Suri
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/241b4b2e87a5410da492bd776acee98a
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spelling	oai:doaj.org-article:241b4b2e87a5410da492bd776acee98a2021-11-18T08:42:57ZSperm associated antigen 9 plays an important role in bladder transitional cell carcinoma.1932-620310.1371/journal.pone.0081348https://doaj.org/article/241b4b2e87a5410da492bd776acee98a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24349057/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Majority of bladder cancer deaths are caused due to transitional cell carcinoma (TCC) which is the most prevalent and chemoresistant malignancy of urinary bladder. Therefore, we analyzed the role of Sperm associated antigen 9 (SPAG9) in bladder TCC.<h4>Methodology and findings</h4>We examined SPAG9 expression and humoral response in 125 bladder TCC patients. Four bladder cancer cell lines were assessed for SPAG9 expression. In addition, we investigated the effect of SPAG9 ablation on cellular proliferation, cell cycle, migration and invasion in UM-UC-3 bladder cancer cells by employing gene silencing approach. Our SPAG9 gene and protein expression analysis revealed SPAG9 expression in 81% of bladder TCC tissue specimens. High SPAG9 expression (>60% SPAG9 positive cells) was found to be significantly associated with superficial non-muscle invasive stage (P = 0.042) and low grade tumors (P = 0.002) suggesting SPAG9 putative role in early spread and tumorigenesis. Humoral response against SPAG9 was observed in 95% of patients found positive for SPAG9 expression. All four bladder cancer cell lines revealed SPAG9 expression. In addition, SPAG9 gene silencing in UM-UC-3 cells resulted in induction of G0-G1 arrest characterized by up-regulation of p16 and p21 and consequent down-regulation of cyclin E, cyclin D and cyclin B, CDK4 and CDK1. Further, SPAG9 gene silencing also resulted in reduction in cellular growth, and migration and invasion ability of cancer cells in vitro.<h4>Conclusions</h4>Collectively, our data in clinical specimens indicated that SPAG9 is potential biomarker and therapeutic target for bladder TCC.Deepika KanojiaManoj GargShikha SainiSumit AgarwalDeepak ParasharNirmala JagadishAmlesh SethAmar BhatnagarAnju GuptaRajive KumarNirmal Kumar LohiyaAnil SuriPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e81348 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Deepika Kanojia Manoj Garg Shikha Saini Sumit Agarwal Deepak Parashar Nirmala Jagadish Amlesh Seth Amar Bhatnagar Anju Gupta Rajive Kumar Nirmal Kumar Lohiya Anil Suri Sperm associated antigen 9 plays an important role in bladder transitional cell carcinoma.
description	<h4>Background</h4>Majority of bladder cancer deaths are caused due to transitional cell carcinoma (TCC) which is the most prevalent and chemoresistant malignancy of urinary bladder. Therefore, we analyzed the role of Sperm associated antigen 9 (SPAG9) in bladder TCC.<h4>Methodology and findings</h4>We examined SPAG9 expression and humoral response in 125 bladder TCC patients. Four bladder cancer cell lines were assessed for SPAG9 expression. In addition, we investigated the effect of SPAG9 ablation on cellular proliferation, cell cycle, migration and invasion in UM-UC-3 bladder cancer cells by employing gene silencing approach. Our SPAG9 gene and protein expression analysis revealed SPAG9 expression in 81% of bladder TCC tissue specimens. High SPAG9 expression (>60% SPAG9 positive cells) was found to be significantly associated with superficial non-muscle invasive stage (P = 0.042) and low grade tumors (P = 0.002) suggesting SPAG9 putative role in early spread and tumorigenesis. Humoral response against SPAG9 was observed in 95% of patients found positive for SPAG9 expression. All four bladder cancer cell lines revealed SPAG9 expression. In addition, SPAG9 gene silencing in UM-UC-3 cells resulted in induction of G0-G1 arrest characterized by up-regulation of p16 and p21 and consequent down-regulation of cyclin E, cyclin D and cyclin B, CDK4 and CDK1. Further, SPAG9 gene silencing also resulted in reduction in cellular growth, and migration and invasion ability of cancer cells in vitro.<h4>Conclusions</h4>Collectively, our data in clinical specimens indicated that SPAG9 is potential biomarker and therapeutic target for bladder TCC.
format	article
author	Deepika Kanojia Manoj Garg Shikha Saini Sumit Agarwal Deepak Parashar Nirmala Jagadish Amlesh Seth Amar Bhatnagar Anju Gupta Rajive Kumar Nirmal Kumar Lohiya Anil Suri
author_facet	Deepika Kanojia Manoj Garg Shikha Saini Sumit Agarwal Deepak Parashar Nirmala Jagadish Amlesh Seth Amar Bhatnagar Anju Gupta Rajive Kumar Nirmal Kumar Lohiya Anil Suri
author_sort	Deepika Kanojia
title	Sperm associated antigen 9 plays an important role in bladder transitional cell carcinoma.
title_short	Sperm associated antigen 9 plays an important role in bladder transitional cell carcinoma.
title_full	Sperm associated antigen 9 plays an important role in bladder transitional cell carcinoma.
title_fullStr	Sperm associated antigen 9 plays an important role in bladder transitional cell carcinoma.
title_full_unstemmed	Sperm associated antigen 9 plays an important role in bladder transitional cell carcinoma.
title_sort	sperm associated antigen 9 plays an important role in bladder transitional cell carcinoma.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/241b4b2e87a5410da492bd776acee98a
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Sperm associated antigen 9 plays an important role in bladder transitional cell carcinoma.

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