Mass spectrometry reveals the chemistry of formaldehyde cross-linking in structured proteins

Formaldehyde (FA) is a popular cross-linking reagent, but applying it for cross-linking mass spectrometry (XLMS) has been largely unsuccessful. Here, the authors show that cross-links in structured proteins are the product of two FA molecules and identify hundreds of FA cross-links by XLMS in vitro...

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Autores principales:	Tamar Tayri-Wilk, Moriya Slavin, Joanna Zamel, Ayelet Blass, Shon Cohen, Alex Motzik, Xue Sun, Deborah E. Shalev, Oren Ram, Nir Kalisman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/241e25d8cf7443558ebf3920295f87b0
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spelling	oai:doaj.org-article:241e25d8cf7443558ebf3920295f87b02021-12-02T17:23:25ZMass spectrometry reveals the chemistry of formaldehyde cross-linking in structured proteins10.1038/s41467-020-16935-w2041-1723https://doaj.org/article/241e25d8cf7443558ebf3920295f87b02020-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16935-whttps://doaj.org/toc/2041-1723Formaldehyde (FA) is a popular cross-linking reagent, but applying it for cross-linking mass spectrometry (XLMS) has been largely unsuccessful. Here, the authors show that cross-links in structured proteins are the product of two FA molecules and identify hundreds of FA cross-links by XLMS in vitro and in situ.Tamar Tayri-WilkMoriya SlavinJoanna ZamelAyelet BlassShon CohenAlex MotzikXue SunDeborah E. ShalevOren RamNir KalismanNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Tamar Tayri-Wilk Moriya Slavin Joanna Zamel Ayelet Blass Shon Cohen Alex Motzik Xue Sun Deborah E. Shalev Oren Ram Nir Kalisman Mass spectrometry reveals the chemistry of formaldehyde cross-linking in structured proteins
description	Formaldehyde (FA) is a popular cross-linking reagent, but applying it for cross-linking mass spectrometry (XLMS) has been largely unsuccessful. Here, the authors show that cross-links in structured proteins are the product of two FA molecules and identify hundreds of FA cross-links by XLMS in vitro and in situ.
format	article
author	Tamar Tayri-Wilk Moriya Slavin Joanna Zamel Ayelet Blass Shon Cohen Alex Motzik Xue Sun Deborah E. Shalev Oren Ram Nir Kalisman
author_facet	Tamar Tayri-Wilk Moriya Slavin Joanna Zamel Ayelet Blass Shon Cohen Alex Motzik Xue Sun Deborah E. Shalev Oren Ram Nir Kalisman
author_sort	Tamar Tayri-Wilk
title	Mass spectrometry reveals the chemistry of formaldehyde cross-linking in structured proteins
title_short	Mass spectrometry reveals the chemistry of formaldehyde cross-linking in structured proteins
title_full	Mass spectrometry reveals the chemistry of formaldehyde cross-linking in structured proteins
title_fullStr	Mass spectrometry reveals the chemistry of formaldehyde cross-linking in structured proteins
title_full_unstemmed	Mass spectrometry reveals the chemistry of formaldehyde cross-linking in structured proteins
title_sort	mass spectrometry reveals the chemistry of formaldehyde cross-linking in structured proteins
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/241e25d8cf7443558ebf3920295f87b0
work_keys_str_mv	AT tamartayriwilk massspectrometryrevealsthechemistryofformaldehydecrosslinkinginstructuredproteins AT moriyaslavin massspectrometryrevealsthechemistryofformaldehydecrosslinkinginstructuredproteins AT joannazamel massspectrometryrevealsthechemistryofformaldehydecrosslinkinginstructuredproteins AT ayeletblass massspectrometryrevealsthechemistryofformaldehydecrosslinkinginstructuredproteins AT shoncohen massspectrometryrevealsthechemistryofformaldehydecrosslinkinginstructuredproteins AT alexmotzik massspectrometryrevealsthechemistryofformaldehydecrosslinkinginstructuredproteins AT xuesun massspectrometryrevealsthechemistryofformaldehydecrosslinkinginstructuredproteins AT deboraheshalev massspectrometryrevealsthechemistryofformaldehydecrosslinkinginstructuredproteins AT orenram massspectrometryrevealsthechemistryofformaldehydecrosslinkinginstructuredproteins AT nirkalisman massspectrometryrevealsthechemistryofformaldehydecrosslinkinginstructuredproteins
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Mass spectrometry reveals the chemistry of formaldehyde cross-linking in structured proteins

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