The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells

The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells

Genetic barcoding has been used to track clonal dynamics of cells. Here, the authors develop a Tracking Recombination Alleles in Clonal Engraftment using sequencing (TRACE-Seq), to barcode repaired alleles by introducing silent mutations or outside of coding regions, to show clonal complexity of edi...

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Autores principales: Rajiv Sharma, Daniel P. Dever, Ciaran M. Lee, Armon Azizi, Yidan Pan, Joab Camarena, Thomas Köhnke, Gang Bao, Matthew H. Porteus, Ravindra Majeti
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/24227f1bebe84818bfcc9151aa481b5d
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spelling oai:doaj.org-article:24227f1bebe84818bfcc9151aa481b5d2021-12-02T14:19:18ZThe TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells10.1038/s41467-020-20792-y2041-1723https://doaj.org/article/24227f1bebe84818bfcc9151aa481b5d2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-20792-yhttps://doaj.org/toc/2041-1723Genetic barcoding has been used to track clonal dynamics of cells. Here, the authors develop a Tracking Recombination Alleles in Clonal Engraftment using sequencing (TRACE-Seq), to barcode repaired alleles by introducing silent mutations or outside of coding regions, to show clonal complexity of edited CD34 + cells following engraftment.Rajiv SharmaDaniel P. DeverCiaran M. LeeArmon AziziYidan PanJoab CamarenaThomas KöhnkeGang BaoMatthew H. PorteusRavindra MajetiNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Rajiv Sharma
Daniel P. Dever
Ciaran M. Lee
Armon Azizi
Yidan Pan
Joab Camarena
Thomas Köhnke
Gang Bao
Matthew H. Porteus
Ravindra Majeti
The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells
description Genetic barcoding has been used to track clonal dynamics of cells. Here, the authors develop a Tracking Recombination Alleles in Clonal Engraftment using sequencing (TRACE-Seq), to barcode repaired alleles by introducing silent mutations or outside of coding regions, to show clonal complexity of edited CD34 + cells following engraftment.
format article
author Rajiv Sharma
Daniel P. Dever
Ciaran M. Lee
Armon Azizi
Yidan Pan
Joab Camarena
Thomas Köhnke
Gang Bao
Matthew H. Porteus
Ravindra Majeti
author_facet Rajiv Sharma
Daniel P. Dever
Ciaran M. Lee
Armon Azizi
Yidan Pan
Joab Camarena
Thomas Köhnke
Gang Bao
Matthew H. Porteus
Ravindra Majeti
author_sort Rajiv Sharma
title The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells
title_short The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells
title_full The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells
title_fullStr The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells
title_full_unstemmed The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells
title_sort trace-seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/24227f1bebe84818bfcc9151aa481b5d
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