Reproducibility of antimicrobial test methods

Abstract We review reproducibility results for methods that test antimicrobial efficacy against biofilms, spores and bacteria dried onto a surface. Our review, that included test results for Pseudomonas aeruginosa, Salmonella choleraesuis and Bacillus subtilis, suggests that the level of reproducibi...

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Autores principales:	Albert E. Parker, Martin A. Hamilton, Darla M. Goeres
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/24274fa833c648cab2e10fbcf4f04b2c
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spelling	oai:doaj.org-article:24274fa833c648cab2e10fbcf4f04b2c2021-12-02T11:40:54ZReproducibility of antimicrobial test methods10.1038/s41598-018-30282-32045-2322https://doaj.org/article/24274fa833c648cab2e10fbcf4f04b2c2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30282-3https://doaj.org/toc/2045-2322Abstract We review reproducibility results for methods that test antimicrobial efficacy against biofilms, spores and bacteria dried onto a surface. Our review, that included test results for Pseudomonas aeruginosa, Salmonella choleraesuis and Bacillus subtilis, suggests that the level of reproducibility depends on the efficacy of the antimicrobial agent being tested for each microbe and microbial environment. To determine the reproducibility of a method, several laboratories must independently test the same antimicrobial agent using the method. Little variability among the efficacy results suggests good reproducibility. Such reproducibility assessments currently are hampered by the absence of an objective process for deciding whether the variability is sufficiently small. We present a quantitative decision process that objectively determines whether any method that assesses antimicrobial efficacy is reproducible. Because the perception of acceptable reproducibility may differ among stakeholders, the decision process is governed by a stakeholder’s specifications that necessarily includes the efficacy of the agents to be tested.Albert E. ParkerMartin A. HamiltonDarla M. GoeresNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Albert E. Parker Martin A. Hamilton Darla M. Goeres Reproducibility of antimicrobial test methods
description	Abstract We review reproducibility results for methods that test antimicrobial efficacy against biofilms, spores and bacteria dried onto a surface. Our review, that included test results for Pseudomonas aeruginosa, Salmonella choleraesuis and Bacillus subtilis, suggests that the level of reproducibility depends on the efficacy of the antimicrobial agent being tested for each microbe and microbial environment. To determine the reproducibility of a method, several laboratories must independently test the same antimicrobial agent using the method. Little variability among the efficacy results suggests good reproducibility. Such reproducibility assessments currently are hampered by the absence of an objective process for deciding whether the variability is sufficiently small. We present a quantitative decision process that objectively determines whether any method that assesses antimicrobial efficacy is reproducible. Because the perception of acceptable reproducibility may differ among stakeholders, the decision process is governed by a stakeholder’s specifications that necessarily includes the efficacy of the agents to be tested.
format	article
author	Albert E. Parker Martin A. Hamilton Darla M. Goeres
author_facet	Albert E. Parker Martin A. Hamilton Darla M. Goeres
author_sort	Albert E. Parker
title	Reproducibility of antimicrobial test methods
title_short	Reproducibility of antimicrobial test methods
title_full	Reproducibility of antimicrobial test methods
title_fullStr	Reproducibility of antimicrobial test methods
title_full_unstemmed	Reproducibility of antimicrobial test methods
title_sort	reproducibility of antimicrobial test methods
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/24274fa833c648cab2e10fbcf4f04b2c
work_keys_str_mv	AT alberteparker reproducibilityofantimicrobialtestmethods AT martinahamilton reproducibilityofantimicrobialtestmethods AT darlamgoeres reproducibilityofantimicrobialtestmethods
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Reproducibility of antimicrobial test methods

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