The structural basis of N-acyl-α-amino-β-lactone formation catalyzed by a nonribosomal peptide synthetase

The antimicrobial β-lactone obafluorin is produced by a Nonribosomal Peptide Synthetase (NRPS). Here the authors present the crystal structure of the obafluorin NRPS and develop a reconstitution assay that allows them to analyse product formation from obafluorin NRPS mutants and alternate substrates...

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Autores principales:	Dale F. Kreitler, Erin M. Gemmell, Jason E. Schaffer, Timothy A. Wencewicz, Andrew M. Gulick
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/2435d7d14da6444cb2bc75c34ae2a3ca
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spelling	oai:doaj.org-article:2435d7d14da6444cb2bc75c34ae2a3ca2021-12-02T16:58:18ZThe structural basis of N-acyl-α-amino-β-lactone formation catalyzed by a nonribosomal peptide synthetase10.1038/s41467-019-11383-72041-1723https://doaj.org/article/2435d7d14da6444cb2bc75c34ae2a3ca2019-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-11383-7https://doaj.org/toc/2041-1723The antimicrobial β-lactone obafluorin is produced by a Nonribosomal Peptide Synthetase (NRPS). Here the authors present the crystal structure of the obafluorin NRPS and develop a reconstitution assay that allows them to analyse product formation from obafluorin NRPS mutants and alternate substrates.Dale F. KreitlerErin M. GemmellJason E. SchafferTimothy A. WencewiczAndrew M. GulickNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Dale F. Kreitler Erin M. Gemmell Jason E. Schaffer Timothy A. Wencewicz Andrew M. Gulick The structural basis of N-acyl-α-amino-β-lactone formation catalyzed by a nonribosomal peptide synthetase
description	The antimicrobial β-lactone obafluorin is produced by a Nonribosomal Peptide Synthetase (NRPS). Here the authors present the crystal structure of the obafluorin NRPS and develop a reconstitution assay that allows them to analyse product formation from obafluorin NRPS mutants and alternate substrates.
format	article
author	Dale F. Kreitler Erin M. Gemmell Jason E. Schaffer Timothy A. Wencewicz Andrew M. Gulick
author_facet	Dale F. Kreitler Erin M. Gemmell Jason E. Schaffer Timothy A. Wencewicz Andrew M. Gulick
author_sort	Dale F. Kreitler
title	The structural basis of N-acyl-α-amino-β-lactone formation catalyzed by a nonribosomal peptide synthetase
title_short	The structural basis of N-acyl-α-amino-β-lactone formation catalyzed by a nonribosomal peptide synthetase
title_full	The structural basis of N-acyl-α-amino-β-lactone formation catalyzed by a nonribosomal peptide synthetase
title_fullStr	The structural basis of N-acyl-α-amino-β-lactone formation catalyzed by a nonribosomal peptide synthetase
title_full_unstemmed	The structural basis of N-acyl-α-amino-β-lactone formation catalyzed by a nonribosomal peptide synthetase
title_sort	structural basis of n-acyl-α-amino-β-lactone formation catalyzed by a nonribosomal peptide synthetase
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/2435d7d14da6444cb2bc75c34ae2a3ca
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The structural basis of N-acyl-α-amino-β-lactone formation catalyzed by a nonribosomal peptide synthetase

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