Electroacupuncture at ST36 Relieves Visceral Hypersensitivity via the NGF/TrkA/TRPV1 Peripheral Afferent Pathway in a Rodent Model of Post-Inflammation Rectal Hypersensitivity

Yan Chen,1,2 Jiafei Cheng,1 Yiling Zhang,1 Jiande DZ Chen,1,3 Florin M Seralu1 1Division of Gastroenterology and Hepatology, Johns Hopkins University School of Medicine, Baltimore, MD, USA; 2Division of Gastroenterology, Binzhou Medical University Hospital, Binzhou, Shandong, People’s Repu...

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Autores principales:	Chen Y, Cheng J, Zhang Y, Chen JDZ, Seralu FM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	electroacupuncture inflammation remission ulcerative colitis visceral hypersensitivity mast cells Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/243707d14fc24a91999d0d1e1940978c
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spelling	oai:doaj.org-article:243707d14fc24a91999d0d1e1940978c2021-12-02T14:14:59ZElectroacupuncture at ST36 Relieves Visceral Hypersensitivity via the NGF/TrkA/TRPV1 Peripheral Afferent Pathway in a Rodent Model of Post-Inflammation Rectal Hypersensitivity1178-7031https://doaj.org/article/243707d14fc24a91999d0d1e1940978c2021-02-01T00:00:00Zhttps://www.dovepress.com/electroacupuncture-at-st36-relieves-visceral-hypersensitivity-via-the--peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Yan Chen,1,2 Jiafei Cheng,1 Yiling Zhang,1 Jiande DZ Chen,1,3 Florin M Seralu1 1Division of Gastroenterology and Hepatology, Johns Hopkins University School of Medicine, Baltimore, MD, USA; 2Division of Gastroenterology, Binzhou Medical University Hospital, Binzhou, Shandong, People’s Republic of China; 3Division of Gastroenterology and Hepatology, University of Michigan, Ann Arbor, MI, USACorrespondence: Florin M SeraluDivision of Gastroenterology and Hepatology, Johns Hopkins University School of Medicine, Baltimore, MD, USATel/Fax +1 4105023147Email fselaru1@jhmi.eduJiande DZ ChenDivision of Gastroenterology and Hepatology, University of Michigan, Ann Arbor, MI, USATel +1 7346475749Email Jiandedzchen@gmail.comPurpose: The aim of the study was to investigate the effects of electroacupuncture (EA) at ST36 on rectal hypersensitivity and compliance in DSS-treated post-inflammation rats. In addition, we explored the involvement of mast cells-triggered NGF/TrkA/TRPV1 peripheral afferent pathway.Methods: Rats were provided water with 5% dextran sulphate sodium (DSS) for 7 days. Two weeks after the DSS treatment they were subjected to initial and repetitive EA. Different sets of parameters were compared in the initial test and then EA with the selected parameters were performed for 2 weeks. Rectal compliance was assessed by colorectal distension while visceral sensitivity was evaluated by abdominal withdraw reflexes (AWR) and electromyogram (EMG). Masson’s trichrome staining was performed to stain collagen and toluidine blue staining was applied to assess the degranulation of mast cells. Nerve growth factor (NGF), tryptase, TrkA and TRPV1 were measured by Western blot or immunofluorescence staining.Results: EA at 100 Hz was more effective in improving rectal compliance and visceral hypersensitivity. Daily EA improved visceral hypersensitivity but not rectal compliance. Five weeks after DSS treatment, fibrosis was noted in both sham-EA and EA groups. The expression and activation of mast cells were significantly reduced after the 2-week EA treatment with a concurrent decrease in the expression of colonic NGF/TrkA and TRPV1 in both colon and dorsal root ganglions.Conclusion: EA at ST36 with a special set of parameters has no effect on reduced rectal compliance but relieves visceral hypersensitivity via the mast cells-triggered NGF/TrkA/TRPV1 peripheral afferent pathway in DSS-treated post-inflammation rats.Keywords: electroacupuncture, inflammation remission, ulcerative colitis, visceral hypersensitivity, mast cellsChen YCheng JZhang YChen JDZSeralu FMDove Medical Pressarticleelectroacupunctureinflammation remissionulcerative colitisvisceral hypersensitivitymast cellsPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 325-339 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	electroacupuncture inflammation remission ulcerative colitis visceral hypersensitivity mast cells Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	electroacupuncture inflammation remission ulcerative colitis visceral hypersensitivity mast cells Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Chen Y Cheng J Zhang Y Chen JDZ Seralu FM Electroacupuncture at ST36 Relieves Visceral Hypersensitivity via the NGF/TrkA/TRPV1 Peripheral Afferent Pathway in a Rodent Model of Post-Inflammation Rectal Hypersensitivity
description	Yan Chen,1,2 Jiafei Cheng,1 Yiling Zhang,1 Jiande DZ Chen,1,3 Florin M Seralu1 1Division of Gastroenterology and Hepatology, Johns Hopkins University School of Medicine, Baltimore, MD, USA; 2Division of Gastroenterology, Binzhou Medical University Hospital, Binzhou, Shandong, People’s Republic of China; 3Division of Gastroenterology and Hepatology, University of Michigan, Ann Arbor, MI, USACorrespondence: Florin M SeraluDivision of Gastroenterology and Hepatology, Johns Hopkins University School of Medicine, Baltimore, MD, USATel/Fax +1 4105023147Email fselaru1@jhmi.eduJiande DZ ChenDivision of Gastroenterology and Hepatology, University of Michigan, Ann Arbor, MI, USATel +1 7346475749Email Jiandedzchen@gmail.comPurpose: The aim of the study was to investigate the effects of electroacupuncture (EA) at ST36 on rectal hypersensitivity and compliance in DSS-treated post-inflammation rats. In addition, we explored the involvement of mast cells-triggered NGF/TrkA/TRPV1 peripheral afferent pathway.Methods: Rats were provided water with 5% dextran sulphate sodium (DSS) for 7 days. Two weeks after the DSS treatment they were subjected to initial and repetitive EA. Different sets of parameters were compared in the initial test and then EA with the selected parameters were performed for 2 weeks. Rectal compliance was assessed by colorectal distension while visceral sensitivity was evaluated by abdominal withdraw reflexes (AWR) and electromyogram (EMG). Masson’s trichrome staining was performed to stain collagen and toluidine blue staining was applied to assess the degranulation of mast cells. Nerve growth factor (NGF), tryptase, TrkA and TRPV1 were measured by Western blot or immunofluorescence staining.Results: EA at 100 Hz was more effective in improving rectal compliance and visceral hypersensitivity. Daily EA improved visceral hypersensitivity but not rectal compliance. Five weeks after DSS treatment, fibrosis was noted in both sham-EA and EA groups. The expression and activation of mast cells were significantly reduced after the 2-week EA treatment with a concurrent decrease in the expression of colonic NGF/TrkA and TRPV1 in both colon and dorsal root ganglions.Conclusion: EA at ST36 with a special set of parameters has no effect on reduced rectal compliance but relieves visceral hypersensitivity via the mast cells-triggered NGF/TrkA/TRPV1 peripheral afferent pathway in DSS-treated post-inflammation rats.Keywords: electroacupuncture, inflammation remission, ulcerative colitis, visceral hypersensitivity, mast cells
format	article
author	Chen Y Cheng J Zhang Y Chen JDZ Seralu FM
author_facet	Chen Y Cheng J Zhang Y Chen JDZ Seralu FM
author_sort	Chen Y
title	Electroacupuncture at ST36 Relieves Visceral Hypersensitivity via the NGF/TrkA/TRPV1 Peripheral Afferent Pathway in a Rodent Model of Post-Inflammation Rectal Hypersensitivity
title_short	Electroacupuncture at ST36 Relieves Visceral Hypersensitivity via the NGF/TrkA/TRPV1 Peripheral Afferent Pathway in a Rodent Model of Post-Inflammation Rectal Hypersensitivity
title_full	Electroacupuncture at ST36 Relieves Visceral Hypersensitivity via the NGF/TrkA/TRPV1 Peripheral Afferent Pathway in a Rodent Model of Post-Inflammation Rectal Hypersensitivity
title_fullStr	Electroacupuncture at ST36 Relieves Visceral Hypersensitivity via the NGF/TrkA/TRPV1 Peripheral Afferent Pathway in a Rodent Model of Post-Inflammation Rectal Hypersensitivity
title_full_unstemmed	Electroacupuncture at ST36 Relieves Visceral Hypersensitivity via the NGF/TrkA/TRPV1 Peripheral Afferent Pathway in a Rodent Model of Post-Inflammation Rectal Hypersensitivity
title_sort	electroacupuncture at st36 relieves visceral hypersensitivity via the ngf/trka/trpv1 peripheral afferent pathway in a rodent model of post-inflammation rectal hypersensitivity
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/243707d14fc24a91999d0d1e1940978c
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Electroacupuncture at ST36 Relieves Visceral Hypersensitivity via the NGF/TrkA/TRPV1 Peripheral Afferent Pathway in a Rodent Model of Post-Inflammation Rectal Hypersensitivity

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