Neurotoxicity of perfluorooctane sulfonate to hippocampal cells in adult mice.

Perfluorooctane sulfonate (PFOS) is a ubiquitous pollutant and found in the environment and in biota. The neurotoxicity of PFOS has received much concern among its various toxic effects when given during developing period of brain. However, little is known about the neurotoxic effects and potential...

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Autores principales:	Yan Long, Yubang Wang, Guixiang Ji, Lifeng Yan, Fan Hu, Aihua Gu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/243840c1a6f8404c85983accaf491b92
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spelling	oai:doaj.org-article:243840c1a6f8404c85983accaf491b922021-11-18T07:59:34ZNeurotoxicity of perfluorooctane sulfonate to hippocampal cells in adult mice.1932-620310.1371/journal.pone.0054176https://doaj.org/article/243840c1a6f8404c85983accaf491b922013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23382877/?tool=EBIhttps://doaj.org/toc/1932-6203Perfluorooctane sulfonate (PFOS) is a ubiquitous pollutant and found in the environment and in biota. The neurotoxicity of PFOS has received much concern among its various toxic effects when given during developing period of brain. However, little is known about the neurotoxic effects and potential mechanisms of PFOS in the mature brain. Our study demonstrated the neurotoxicity and the potential mechanisms of PFOS in the hippocampus of adult mice for the first time. The impairments of spatial learning and memory were observed by water maze studies after exposure to PFOS for three months. Significant apoptosis was found in hippocampal cells after PFOS exposure, accompanied with a increase of glutamate in the hippocampus and decreases of dopamine (DA) and 3,4-dihydrophenylacetic acid (DOPAC) in Caudate Putamen in the 10.75 mg/kg PFOS group. By two-dimensional fluorescence difference in gel electrophoresis (2D-DIGE) analysis, seven related proteins in the hippocampus that responded to PFOS exposure were identified, among which, Mib1 protein (an E3 ubiquitin-protein ligase), Herc5 (hect domain and RLD 5 isoform 2) and Tyro3 (TYRO3 protein tyrosine kinase 3) were found down-regulated, while Sdha (Succinate dehydrogenase flavoprotein subunit), Gzma (Isoform HF1 of Granzyme A precursor), Plau (Urokinase-type plasminogen activator precursor) and Lig4 (DNA ligase 4) were found up-regulated in the 10.75 mg/kg PFOS-treated group compare with control group. Furthermore, we also found that (i) increased expression of caspase-3 protein and decreased expression of Bcl-2, Bcl-XL and survivin proteins, (ii) the increased glutamate release in the hippocampus. All these might contribute to the dysfunction of hippocampus which finally account for the impairments of spatial learning and memory in adult mice.Yan LongYubang WangGuixiang JiLifeng YanFan HuAihua GuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e54176 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yan Long Yubang Wang Guixiang Ji Lifeng Yan Fan Hu Aihua Gu Neurotoxicity of perfluorooctane sulfonate to hippocampal cells in adult mice.
description	Perfluorooctane sulfonate (PFOS) is a ubiquitous pollutant and found in the environment and in biota. The neurotoxicity of PFOS has received much concern among its various toxic effects when given during developing period of brain. However, little is known about the neurotoxic effects and potential mechanisms of PFOS in the mature brain. Our study demonstrated the neurotoxicity and the potential mechanisms of PFOS in the hippocampus of adult mice for the first time. The impairments of spatial learning and memory were observed by water maze studies after exposure to PFOS for three months. Significant apoptosis was found in hippocampal cells after PFOS exposure, accompanied with a increase of glutamate in the hippocampus and decreases of dopamine (DA) and 3,4-dihydrophenylacetic acid (DOPAC) in Caudate Putamen in the 10.75 mg/kg PFOS group. By two-dimensional fluorescence difference in gel electrophoresis (2D-DIGE) analysis, seven related proteins in the hippocampus that responded to PFOS exposure were identified, among which, Mib1 protein (an E3 ubiquitin-protein ligase), Herc5 (hect domain and RLD 5 isoform 2) and Tyro3 (TYRO3 protein tyrosine kinase 3) were found down-regulated, while Sdha (Succinate dehydrogenase flavoprotein subunit), Gzma (Isoform HF1 of Granzyme A precursor), Plau (Urokinase-type plasminogen activator precursor) and Lig4 (DNA ligase 4) were found up-regulated in the 10.75 mg/kg PFOS-treated group compare with control group. Furthermore, we also found that (i) increased expression of caspase-3 protein and decreased expression of Bcl-2, Bcl-XL and survivin proteins, (ii) the increased glutamate release in the hippocampus. All these might contribute to the dysfunction of hippocampus which finally account for the impairments of spatial learning and memory in adult mice.
format	article
author	Yan Long Yubang Wang Guixiang Ji Lifeng Yan Fan Hu Aihua Gu
author_facet	Yan Long Yubang Wang Guixiang Ji Lifeng Yan Fan Hu Aihua Gu
author_sort	Yan Long
title	Neurotoxicity of perfluorooctane sulfonate to hippocampal cells in adult mice.
title_short	Neurotoxicity of perfluorooctane sulfonate to hippocampal cells in adult mice.
title_full	Neurotoxicity of perfluorooctane sulfonate to hippocampal cells in adult mice.
title_fullStr	Neurotoxicity of perfluorooctane sulfonate to hippocampal cells in adult mice.
title_full_unstemmed	Neurotoxicity of perfluorooctane sulfonate to hippocampal cells in adult mice.
title_sort	neurotoxicity of perfluorooctane sulfonate to hippocampal cells in adult mice.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/243840c1a6f8404c85983accaf491b92
work_keys_str_mv	AT yanlong neurotoxicityofperfluorooctanesulfonatetohippocampalcellsinadultmice AT yubangwang neurotoxicityofperfluorooctanesulfonatetohippocampalcellsinadultmice AT guixiangji neurotoxicityofperfluorooctanesulfonatetohippocampalcellsinadultmice AT lifengyan neurotoxicityofperfluorooctanesulfonatetohippocampalcellsinadultmice AT fanhu neurotoxicityofperfluorooctanesulfonatetohippocampalcellsinadultmice AT aihuagu neurotoxicityofperfluorooctanesulfonatetohippocampalcellsinadultmice
_version_	1718422653650337792

Neurotoxicity of perfluorooctane sulfonate to hippocampal cells in adult mice.

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