Super-resolution imaging using nano-bells

Abstract In this paper we demonstrate a new scheme for optical super-resolution, inspired, in-part, by PALM and STORM. In this scheme each object in the field of view is tagged with a signal that allows them to be detected separately. By doing this we can identify and locate each object separately w...

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Autores principales:	Rafael Fuentes-Domínguez, Fernando Pérez-Cota, Shakila Naznin, Richard J. Smith, Matt Clark
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Super Resolution Techniques Optical PSF Optical Resolution Limit Point Spread Function (PSF) Gridded Coverslips Medicine R Science Q
Acceso en línea:	https://doaj.org/article/243c062c7ff24562b36fde979a6e1a0a
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spelling	oai:doaj.org-article:243c062c7ff24562b36fde979a6e1a0a2021-12-02T15:08:52ZSuper-resolution imaging using nano-bells10.1038/s41598-018-34744-62045-2322https://doaj.org/article/243c062c7ff24562b36fde979a6e1a0a2018-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34744-6https://doaj.org/toc/2045-2322Abstract In this paper we demonstrate a new scheme for optical super-resolution, inspired, in-part, by PALM and STORM. In this scheme each object in the field of view is tagged with a signal that allows them to be detected separately. By doing this we can identify and locate each object separately with significantly higher resolution than the diffraction limit. We demonstrate this by imaging nanoparticles significantly smaller than the optical resolution limit. In this case the “tag” we have used is the frequency of vibration of nanoscale “bells” made of metallic nanoparticles whose acoustic vibrational frequency is in the multi-GHz range. Since the vibration of the particles can be easily excited and detected and the frequency is directly related to the particle size, we can separate the signals from many particles of sufficiently different sizes even though they are smaller than, and separated by less than, the optical resolution limit. Using this scheme we have been able to localise the nanoparticle position with a precision of ~3 nm. This has many potential advantages - such nanoparticles are easily inserted into cells and well tolerated, the particles do not bleach and can be produced easily with very dispersed sizes. We estimate that 50 or more different particles (or frequency channels) can be accessed in each optical point spread function using the vibrational frequencies of gold nanospheres. However, many more channels may be accessed using more complex structures (such as nanorods) and detection techniques (for instance using polarization or wavelength selective detection) opening up this technique as a generalized method of achieving super-optical resolution imaging.Rafael Fuentes-DomínguezFernando Pérez-CotaShakila NazninRichard J. SmithMatt ClarkNature PortfolioarticleSuper Resolution TechniquesOptical PSFOptical Resolution LimitPoint Spread Function (PSF)Gridded CoverslipsMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Super Resolution Techniques Optical PSF Optical Resolution Limit Point Spread Function (PSF) Gridded Coverslips Medicine R Science Q
spellingShingle	Super Resolution Techniques Optical PSF Optical Resolution Limit Point Spread Function (PSF) Gridded Coverslips Medicine R Science Q Rafael Fuentes-Domínguez Fernando Pérez-Cota Shakila Naznin Richard J. Smith Matt Clark Super-resolution imaging using nano-bells
description	Abstract In this paper we demonstrate a new scheme for optical super-resolution, inspired, in-part, by PALM and STORM. In this scheme each object in the field of view is tagged with a signal that allows them to be detected separately. By doing this we can identify and locate each object separately with significantly higher resolution than the diffraction limit. We demonstrate this by imaging nanoparticles significantly smaller than the optical resolution limit. In this case the “tag” we have used is the frequency of vibration of nanoscale “bells” made of metallic nanoparticles whose acoustic vibrational frequency is in the multi-GHz range. Since the vibration of the particles can be easily excited and detected and the frequency is directly related to the particle size, we can separate the signals from many particles of sufficiently different sizes even though they are smaller than, and separated by less than, the optical resolution limit. Using this scheme we have been able to localise the nanoparticle position with a precision of ~3 nm. This has many potential advantages - such nanoparticles are easily inserted into cells and well tolerated, the particles do not bleach and can be produced easily with very dispersed sizes. We estimate that 50 or more different particles (or frequency channels) can be accessed in each optical point spread function using the vibrational frequencies of gold nanospheres. However, many more channels may be accessed using more complex structures (such as nanorods) and detection techniques (for instance using polarization or wavelength selective detection) opening up this technique as a generalized method of achieving super-optical resolution imaging.
format	article
author	Rafael Fuentes-Domínguez Fernando Pérez-Cota Shakila Naznin Richard J. Smith Matt Clark
author_facet	Rafael Fuentes-Domínguez Fernando Pérez-Cota Shakila Naznin Richard J. Smith Matt Clark
author_sort	Rafael Fuentes-Domínguez
title	Super-resolution imaging using nano-bells
title_short	Super-resolution imaging using nano-bells
title_full	Super-resolution imaging using nano-bells
title_fullStr	Super-resolution imaging using nano-bells
title_full_unstemmed	Super-resolution imaging using nano-bells
title_sort	super-resolution imaging using nano-bells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/243c062c7ff24562b36fde979a6e1a0a
work_keys_str_mv	AT rafaelfuentesdominguez superresolutionimagingusingnanobells AT fernandoperezcota superresolutionimagingusingnanobells AT shakilanaznin superresolutionimagingusingnanobells AT richardjsmith superresolutionimagingusingnanobells AT mattclark superresolutionimagingusingnanobells
_version_	1718387988706099200

Super-resolution imaging using nano-bells

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