Distinct expression profiles and different functions of odorant binding proteins in Nilaparvata lugens Stål.

<h4>Background</h4>Odorant binding proteins (OBPs) play important roles in insect olfaction. The brown planthopper (BPH), Nilaparvata lugens Stål (Delphacidae, Auchenorrhyncha, Hemiptera) is one of the most important rice pests. Its monophagy (only feeding on rice), wing form (long and s...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Peng He, Jin Zhang, Nai-Yong Liu, Ya-Nan Zhang, Ke Yang, Shuang-Lin Dong
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/243d524589334534b31e94cc6460d6d3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:243d524589334534b31e94cc6460d6d3
record_format	dspace
spelling	oai:doaj.org-article:243d524589334534b31e94cc6460d6d32021-11-18T07:32:34ZDistinct expression profiles and different functions of odorant binding proteins in Nilaparvata lugens Stål.1932-620310.1371/journal.pone.0028921https://doaj.org/article/243d524589334534b31e94cc6460d6d32011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22174925/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Odorant binding proteins (OBPs) play important roles in insect olfaction. The brown planthopper (BPH), Nilaparvata lugens Stål (Delphacidae, Auchenorrhyncha, Hemiptera) is one of the most important rice pests. Its monophagy (only feeding on rice), wing form (long and short wing) variation, and annual long distance migration (seeking for rice plants of high nutrition) imply that the olfaction would play a central role in BPH behavior. However, the olfaction related proteins have not been characterized in this insect.<h4>Methodology/principal findings</h4>Full length cDNA of three OBPs were obtained and distinct expression profiles were revealed regarding to tissue, developmental stage, wing form and gender for the first time for the species. The results provide important clues in functional differentiation of these genes. Binding assays with 41 compounds demonstrated that NlugOBP3 had markedly higher binding ability and wider binding spectrum than the other two OBPs. Terpenes and Ketones displayed higher binding while Alkanes showed no binding to the three OBPs. Focused on NlugOBP3, RNA interference experiments showed that NlugOBP3 not only involved in nymph olfaction on rice seedlings, but also had non-olfactory functions, as it was closely related to nymph survival.<h4>Conclusions</h4>NlugOBP3 plays important roles in both olfaction and survival of BPH. It may serve as a potential target for developing behavioral disruptant and/or lethal agent in N. lugens.Peng HeJin ZhangNai-Yong LiuYa-Nan ZhangKe YangShuang-Lin DongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e28921 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peng He Jin Zhang Nai-Yong Liu Ya-Nan Zhang Ke Yang Shuang-Lin Dong Distinct expression profiles and different functions of odorant binding proteins in Nilaparvata lugens Stål.
description	<h4>Background</h4>Odorant binding proteins (OBPs) play important roles in insect olfaction. The brown planthopper (BPH), Nilaparvata lugens Stål (Delphacidae, Auchenorrhyncha, Hemiptera) is one of the most important rice pests. Its monophagy (only feeding on rice), wing form (long and short wing) variation, and annual long distance migration (seeking for rice plants of high nutrition) imply that the olfaction would play a central role in BPH behavior. However, the olfaction related proteins have not been characterized in this insect.<h4>Methodology/principal findings</h4>Full length cDNA of three OBPs were obtained and distinct expression profiles were revealed regarding to tissue, developmental stage, wing form and gender for the first time for the species. The results provide important clues in functional differentiation of these genes. Binding assays with 41 compounds demonstrated that NlugOBP3 had markedly higher binding ability and wider binding spectrum than the other two OBPs. Terpenes and Ketones displayed higher binding while Alkanes showed no binding to the three OBPs. Focused on NlugOBP3, RNA interference experiments showed that NlugOBP3 not only involved in nymph olfaction on rice seedlings, but also had non-olfactory functions, as it was closely related to nymph survival.<h4>Conclusions</h4>NlugOBP3 plays important roles in both olfaction and survival of BPH. It may serve as a potential target for developing behavioral disruptant and/or lethal agent in N. lugens.
format	article
author	Peng He Jin Zhang Nai-Yong Liu Ya-Nan Zhang Ke Yang Shuang-Lin Dong
author_facet	Peng He Jin Zhang Nai-Yong Liu Ya-Nan Zhang Ke Yang Shuang-Lin Dong
author_sort	Peng He
title	Distinct expression profiles and different functions of odorant binding proteins in Nilaparvata lugens Stål.
title_short	Distinct expression profiles and different functions of odorant binding proteins in Nilaparvata lugens Stål.
title_full	Distinct expression profiles and different functions of odorant binding proteins in Nilaparvata lugens Stål.
title_fullStr	Distinct expression profiles and different functions of odorant binding proteins in Nilaparvata lugens Stål.
title_full_unstemmed	Distinct expression profiles and different functions of odorant binding proteins in Nilaparvata lugens Stål.
title_sort	distinct expression profiles and different functions of odorant binding proteins in nilaparvata lugens stål.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/243d524589334534b31e94cc6460d6d3
work_keys_str_mv	AT penghe distinctexpressionprofilesanddifferentfunctionsofodorantbindingproteinsinnilaparvatalugensstal AT jinzhang distinctexpressionprofilesanddifferentfunctionsofodorantbindingproteinsinnilaparvatalugensstal AT naiyongliu distinctexpressionprofilesanddifferentfunctionsofodorantbindingproteinsinnilaparvatalugensstal AT yananzhang distinctexpressionprofilesanddifferentfunctionsofodorantbindingproteinsinnilaparvatalugensstal AT keyang distinctexpressionprofilesanddifferentfunctionsofodorantbindingproteinsinnilaparvatalugensstal AT shuanglindong distinctexpressionprofilesanddifferentfunctionsofodorantbindingproteinsinnilaparvatalugensstal
_version_	1718423273044180992

Distinct expression profiles and different functions of odorant binding proteins in Nilaparvata lugens Stål.

Ejemplares similares