TRPM8 channel inhibitor-encapsulated hydrogel as a tunable surface for bone tissue engineering

Abstract A major limitation in the bio-medical sector is the availability of materials suitable for bone tissue engineering using stem cells and methodology converting the stochastic biological events towards definitive as well as efficient bio-mineralization. We show that osteoblasts and Bone Marro...

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Autores principales:	Tusar Kanta Acharya, Satish Kumar, Nikhil Tiwari, Arijit Ghosh, Ankit Tiwari, Subhashis Pal, Rakesh Kumar Majhi, Ashutosh Kumar, Rashmita Das, Abhishek Singh, Pradip K. Maji, Naibedya Chattopadhyay, Luna Goswami, Chandan Goswami
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/244af8698191426da6e6ef3ea0c07e0a
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spelling	oai:doaj.org-article:244af8698191426da6e6ef3ea0c07e0a2021-12-02T13:30:17ZTRPM8 channel inhibitor-encapsulated hydrogel as a tunable surface for bone tissue engineering10.1038/s41598-021-81041-w2045-2322https://doaj.org/article/244af8698191426da6e6ef3ea0c07e0a2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81041-whttps://doaj.org/toc/2045-2322Abstract A major limitation in the bio-medical sector is the availability of materials suitable for bone tissue engineering using stem cells and methodology converting the stochastic biological events towards definitive as well as efficient bio-mineralization. We show that osteoblasts and Bone Marrow-derived Mesenchymal Stem Cell Pools (BM-MSCP) express TRPM8, a Ca2+-ion channel critical for bone-mineralization. TRPM8 inhibition triggers up-regulation of key osteogenesis factors; and increases mineralization by osteoblasts. We utilized CMT:HEMA, a carbohydrate polymer-based hydrogel that has nanofiber-like structure suitable for optimum delivery of TRPM8-specific activators or inhibitors. This hydrogel is ideal for proper adhesion, growth, and differentiation of osteoblast cell lines, primary osteoblasts, and BM-MSCP. CMT:HEMA coated with AMTB (TRPM8 inhibitor) induces differentiation of BM-MSCP into osteoblasts and subsequent mineralization in a dose-dependent manner. Prolonged and optimum inhibition of TRPM8 by AMTB released from the gels results in upregulation of osteogenic markers. We propose that AMTB-coated CMT:HEMA can be used as a tunable surface for bone tissue engineering. These findings may have broad implications in different bio-medical sectors.Tusar Kanta AcharyaSatish KumarNikhil TiwariArijit GhoshAnkit TiwariSubhashis PalRakesh Kumar MajhiAshutosh KumarRashmita DasAbhishek SinghPradip K. MajiNaibedya ChattopadhyayLuna GoswamiChandan GoswamiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tusar Kanta Acharya Satish Kumar Nikhil Tiwari Arijit Ghosh Ankit Tiwari Subhashis Pal Rakesh Kumar Majhi Ashutosh Kumar Rashmita Das Abhishek Singh Pradip K. Maji Naibedya Chattopadhyay Luna Goswami Chandan Goswami TRPM8 channel inhibitor-encapsulated hydrogel as a tunable surface for bone tissue engineering
description	Abstract A major limitation in the bio-medical sector is the availability of materials suitable for bone tissue engineering using stem cells and methodology converting the stochastic biological events towards definitive as well as efficient bio-mineralization. We show that osteoblasts and Bone Marrow-derived Mesenchymal Stem Cell Pools (BM-MSCP) express TRPM8, a Ca2+-ion channel critical for bone-mineralization. TRPM8 inhibition triggers up-regulation of key osteogenesis factors; and increases mineralization by osteoblasts. We utilized CMT:HEMA, a carbohydrate polymer-based hydrogel that has nanofiber-like structure suitable for optimum delivery of TRPM8-specific activators or inhibitors. This hydrogel is ideal for proper adhesion, growth, and differentiation of osteoblast cell lines, primary osteoblasts, and BM-MSCP. CMT:HEMA coated with AMTB (TRPM8 inhibitor) induces differentiation of BM-MSCP into osteoblasts and subsequent mineralization in a dose-dependent manner. Prolonged and optimum inhibition of TRPM8 by AMTB released from the gels results in upregulation of osteogenic markers. We propose that AMTB-coated CMT:HEMA can be used as a tunable surface for bone tissue engineering. These findings may have broad implications in different bio-medical sectors.
format	article
author	Tusar Kanta Acharya Satish Kumar Nikhil Tiwari Arijit Ghosh Ankit Tiwari Subhashis Pal Rakesh Kumar Majhi Ashutosh Kumar Rashmita Das Abhishek Singh Pradip K. Maji Naibedya Chattopadhyay Luna Goswami Chandan Goswami
author_facet	Tusar Kanta Acharya Satish Kumar Nikhil Tiwari Arijit Ghosh Ankit Tiwari Subhashis Pal Rakesh Kumar Majhi Ashutosh Kumar Rashmita Das Abhishek Singh Pradip K. Maji Naibedya Chattopadhyay Luna Goswami Chandan Goswami
author_sort	Tusar Kanta Acharya
title	TRPM8 channel inhibitor-encapsulated hydrogel as a tunable surface for bone tissue engineering
title_short	TRPM8 channel inhibitor-encapsulated hydrogel as a tunable surface for bone tissue engineering
title_full	TRPM8 channel inhibitor-encapsulated hydrogel as a tunable surface for bone tissue engineering
title_fullStr	TRPM8 channel inhibitor-encapsulated hydrogel as a tunable surface for bone tissue engineering
title_full_unstemmed	TRPM8 channel inhibitor-encapsulated hydrogel as a tunable surface for bone tissue engineering
title_sort	trpm8 channel inhibitor-encapsulated hydrogel as a tunable surface for bone tissue engineering
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/244af8698191426da6e6ef3ea0c07e0a
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TRPM8 channel inhibitor-encapsulated hydrogel as a tunable surface for bone tissue engineering

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