Formation of the embryonic organizer is restricted by the competitive influences of Fgf signaling and the SoxB1 transcription factors.

The organizer is one of the earliest structures to be established during vertebrate development and is crucial to subsequent patterning of the embryo. We have previously shown that the SoxB1 transcription factor, Sox3, plays a central role as a transcriptional repressor of zebrafish organizer gene e...

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Autores principales:	Cheng-Liang Kuo, Chi Man Lam, Jane E Hewitt, Paul J Scotting
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/244e743c9df54675bd79434aa7ef1ea4
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spelling	oai:doaj.org-article:244e743c9df54675bd79434aa7ef1ea42021-11-18T07:55:25ZFormation of the embryonic organizer is restricted by the competitive influences of Fgf signaling and the SoxB1 transcription factors.1932-620310.1371/journal.pone.0057698https://doaj.org/article/244e743c9df54675bd79434aa7ef1ea42013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23469052/?tool=EBIhttps://doaj.org/toc/1932-6203The organizer is one of the earliest structures to be established during vertebrate development and is crucial to subsequent patterning of the embryo. We have previously shown that the SoxB1 transcription factor, Sox3, plays a central role as a transcriptional repressor of zebrafish organizer gene expression. Recent data suggest that Fgf signaling has a positive influence on organizer formation, but its role remains to be fully elucidated. In order to better understand how Fgf signaling fits into the complex regulatory network that determines when and where the organizer forms, the relationship between the positive effects of Fgf signaling and the repressive effects of the SoxB1 factors must be resolved. This study demonstrates that both fgf3 and fgf8 are required for expression of the organizer genes, gsc and chd, and that SoxB1 factors (Sox3, and the zebrafish specific factors, Sox19a and Sox19b) can repress the expression of both fgf3 and fgf8. However, we also find that these SoxB1 factors inhibit the expression of gsc and chd independently of their repression of fgf expression. We show that ectopic expression of organizer genes induced solely by the inhibition of SoxB1 function is dependent upon the activation of fgf expression. These data allow us to describe a comprehensive signaling network in which the SoxB1 factors restrict organizer formation by inhibiting Fgf, Nodal and Wnt signaling, as well as independently repressing the targets of that signaling. The organizer therefore forms only where Nodal-induced Fgf signaling overlaps with Wnt signaling and the SoxB1 proteins are absent.Cheng-Liang KuoChi Man LamJane E HewittPaul J ScottingPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e57698 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cheng-Liang Kuo Chi Man Lam Jane E Hewitt Paul J Scotting Formation of the embryonic organizer is restricted by the competitive influences of Fgf signaling and the SoxB1 transcription factors.
description	The organizer is one of the earliest structures to be established during vertebrate development and is crucial to subsequent patterning of the embryo. We have previously shown that the SoxB1 transcription factor, Sox3, plays a central role as a transcriptional repressor of zebrafish organizer gene expression. Recent data suggest that Fgf signaling has a positive influence on organizer formation, but its role remains to be fully elucidated. In order to better understand how Fgf signaling fits into the complex regulatory network that determines when and where the organizer forms, the relationship between the positive effects of Fgf signaling and the repressive effects of the SoxB1 factors must be resolved. This study demonstrates that both fgf3 and fgf8 are required for expression of the organizer genes, gsc and chd, and that SoxB1 factors (Sox3, and the zebrafish specific factors, Sox19a and Sox19b) can repress the expression of both fgf3 and fgf8. However, we also find that these SoxB1 factors inhibit the expression of gsc and chd independently of their repression of fgf expression. We show that ectopic expression of organizer genes induced solely by the inhibition of SoxB1 function is dependent upon the activation of fgf expression. These data allow us to describe a comprehensive signaling network in which the SoxB1 factors restrict organizer formation by inhibiting Fgf, Nodal and Wnt signaling, as well as independently repressing the targets of that signaling. The organizer therefore forms only where Nodal-induced Fgf signaling overlaps with Wnt signaling and the SoxB1 proteins are absent.
format	article
author	Cheng-Liang Kuo Chi Man Lam Jane E Hewitt Paul J Scotting
author_facet	Cheng-Liang Kuo Chi Man Lam Jane E Hewitt Paul J Scotting
author_sort	Cheng-Liang Kuo
title	Formation of the embryonic organizer is restricted by the competitive influences of Fgf signaling and the SoxB1 transcription factors.
title_short	Formation of the embryonic organizer is restricted by the competitive influences of Fgf signaling and the SoxB1 transcription factors.
title_full	Formation of the embryonic organizer is restricted by the competitive influences of Fgf signaling and the SoxB1 transcription factors.
title_fullStr	Formation of the embryonic organizer is restricted by the competitive influences of Fgf signaling and the SoxB1 transcription factors.
title_full_unstemmed	Formation of the embryonic organizer is restricted by the competitive influences of Fgf signaling and the SoxB1 transcription factors.
title_sort	formation of the embryonic organizer is restricted by the competitive influences of fgf signaling and the soxb1 transcription factors.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/244e743c9df54675bd79434aa7ef1ea4
work_keys_str_mv	AT chengliangkuo formationoftheembryonicorganizerisrestrictedbythecompetitiveinfluencesoffgfsignalingandthesoxb1transcriptionfactors AT chimanlam formationoftheembryonicorganizerisrestrictedbythecompetitiveinfluencesoffgfsignalingandthesoxb1transcriptionfactors AT janeehewitt formationoftheembryonicorganizerisrestrictedbythecompetitiveinfluencesoffgfsignalingandthesoxb1transcriptionfactors AT pauljscotting formationoftheembryonicorganizerisrestrictedbythecompetitiveinfluencesoffgfsignalingandthesoxb1transcriptionfactors
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Formation of the embryonic organizer is restricted by the competitive influences of Fgf signaling and the SoxB1 transcription factors.

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