Impaired renal function and dysbiosis of gut microbiota contribute to increased trimethylamine-N-oxide in chronic kidney disease patients

Abstract Chronic kidney disease (CKD) patients have an increased risk of cardiovascular diseases (CVDs). The present study aimed to investigate the gut microbiota and blood trimethylamine-N-oxide concentration (TMAO) in Chinese CKD patients and explore the underlying explanations through the animal...

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Autores principales:	Kai-Yu Xu, Geng-Hong Xia, Jun-Qi Lu, Mu-Xuan Chen, Xin Zhen, Shan Wang, Chao You, Jing Nie, Hong-Wei Zhou, Jia Yin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2451301a7e214c35a44a1aaea8db50ff
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spelling	oai:doaj.org-article:2451301a7e214c35a44a1aaea8db50ff2021-12-02T11:53:13ZImpaired renal function and dysbiosis of gut microbiota contribute to increased trimethylamine-N-oxide in chronic kidney disease patients10.1038/s41598-017-01387-y2045-2322https://doaj.org/article/2451301a7e214c35a44a1aaea8db50ff2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01387-yhttps://doaj.org/toc/2045-2322Abstract Chronic kidney disease (CKD) patients have an increased risk of cardiovascular diseases (CVDs). The present study aimed to investigate the gut microbiota and blood trimethylamine-N-oxide concentration (TMAO) in Chinese CKD patients and explore the underlying explanations through the animal experiment. The median plasma TMAO level was 30.33 μmol/L in the CKD patients, which was significantly higher than the 2.08 μmol/L concentration measured in the healthy controls. Next-generation sequence revealed obvious dysbiosis of the gut microbiome in CKD patients, with reduced bacterial diversity and biased community constitutions. CKD patients had higher percentages of opportunistic pathogens from gamma-Proteobacteria and reduced percentages of beneficial microbes, such as Roseburia, Coprococcus, and Ruminococcaceae. The PICRUSt analysis demonstrated that eight genes involved in choline, betaine, L-carnitine and trimethylamine (TMA) metabolism were changed in the CKD patients. Moreover, we transferred faecal samples from CKD patients and healthy controls into antibiotic-treated C57BL/6 mice and found that the mice that received gut microbes from the CKD patients had significantly higher plasma TMAO levels and different composition of gut microbiota than did the comparative mouse group. Our present study demonstrated that CKD patients had increased plasma TMAO levels due to contributions from both impaired renal functions and dysbiosis of the gut microbiota.Kai-Yu XuGeng-Hong XiaJun-Qi LuMu-Xuan ChenXin ZhenShan WangChao YouJing NieHong-Wei ZhouJia YinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kai-Yu Xu Geng-Hong Xia Jun-Qi Lu Mu-Xuan Chen Xin Zhen Shan Wang Chao You Jing Nie Hong-Wei Zhou Jia Yin Impaired renal function and dysbiosis of gut microbiota contribute to increased trimethylamine-N-oxide in chronic kidney disease patients
description	Abstract Chronic kidney disease (CKD) patients have an increased risk of cardiovascular diseases (CVDs). The present study aimed to investigate the gut microbiota and blood trimethylamine-N-oxide concentration (TMAO) in Chinese CKD patients and explore the underlying explanations through the animal experiment. The median plasma TMAO level was 30.33 μmol/L in the CKD patients, which was significantly higher than the 2.08 μmol/L concentration measured in the healthy controls. Next-generation sequence revealed obvious dysbiosis of the gut microbiome in CKD patients, with reduced bacterial diversity and biased community constitutions. CKD patients had higher percentages of opportunistic pathogens from gamma-Proteobacteria and reduced percentages of beneficial microbes, such as Roseburia, Coprococcus, and Ruminococcaceae. The PICRUSt analysis demonstrated that eight genes involved in choline, betaine, L-carnitine and trimethylamine (TMA) metabolism were changed in the CKD patients. Moreover, we transferred faecal samples from CKD patients and healthy controls into antibiotic-treated C57BL/6 mice and found that the mice that received gut microbes from the CKD patients had significantly higher plasma TMAO levels and different composition of gut microbiota than did the comparative mouse group. Our present study demonstrated that CKD patients had increased plasma TMAO levels due to contributions from both impaired renal functions and dysbiosis of the gut microbiota.
format	article
author	Kai-Yu Xu Geng-Hong Xia Jun-Qi Lu Mu-Xuan Chen Xin Zhen Shan Wang Chao You Jing Nie Hong-Wei Zhou Jia Yin
author_facet	Kai-Yu Xu Geng-Hong Xia Jun-Qi Lu Mu-Xuan Chen Xin Zhen Shan Wang Chao You Jing Nie Hong-Wei Zhou Jia Yin
author_sort	Kai-Yu Xu
title	Impaired renal function and dysbiosis of gut microbiota contribute to increased trimethylamine-N-oxide in chronic kidney disease patients
title_short	Impaired renal function and dysbiosis of gut microbiota contribute to increased trimethylamine-N-oxide in chronic kidney disease patients
title_full	Impaired renal function and dysbiosis of gut microbiota contribute to increased trimethylamine-N-oxide in chronic kidney disease patients
title_fullStr	Impaired renal function and dysbiosis of gut microbiota contribute to increased trimethylamine-N-oxide in chronic kidney disease patients
title_full_unstemmed	Impaired renal function and dysbiosis of gut microbiota contribute to increased trimethylamine-N-oxide in chronic kidney disease patients
title_sort	impaired renal function and dysbiosis of gut microbiota contribute to increased trimethylamine-n-oxide in chronic kidney disease patients
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2451301a7e214c35a44a1aaea8db50ff
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Impaired renal function and dysbiosis of gut microbiota contribute to increased trimethylamine-N-oxide in chronic kidney disease patients

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