Nanostring-Based Identification of the Gene Expression Profile in Trigger Finger Samples

Trigger finger is a common yet vastly understudied fibroproliferative hand pathology, severely affecting patients’ quality of life. Consistent trauma due to inadequate positioning within the afflicted finger’s tendon/pulley system leads to cellular dysregulation and eventual fibrosis. While the gene...

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Autores principales:	Ravindra Kolhe, Umar Ghilzai, Ashis K. Mondal, Chetan Pundkar, Pankaj Ahluwalia, Nikhil S. Sahajpal, Jie Chen, Carlos M. Isales, Mark Fulcher, Sadanand Fulzele
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	trigger finger pain gene expression Nanostring Medicine R
Acceso en línea:	https://doaj.org/article/24515fc1811c4fd488a6ea56a11c1370
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spelling	oai:doaj.org-article:24515fc1811c4fd488a6ea56a11c13702021-11-25T17:46:44ZNanostring-Based Identification of the Gene Expression Profile in Trigger Finger Samples10.3390/healthcare91115922227-9032https://doaj.org/article/24515fc1811c4fd488a6ea56a11c13702021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9032/9/11/1592https://doaj.org/toc/2227-9032Trigger finger is a common yet vastly understudied fibroproliferative hand pathology, severely affecting patients’ quality of life. Consistent trauma due to inadequate positioning within the afflicted finger’s tendon/pulley system leads to cellular dysregulation and eventual fibrosis. While the genetic characteristics of the fibrotic tissue in the trigger finger have been studied, the pathways that govern the initiation and propagation of fibrosis are still unknown. The complete gene expression profile of the trigger finger has never been explored. Our study has used the Nanostring nCounter gene expression assay to investigate the molecular signaling involved in trigger finger pathogenesis. We collected samples from patients undergoing trigger finger (<i>n</i> = 4) release surgery and compared the gene expression to carpal tunnel tissue (<i>n</i> = 4). Nanostring nCounter analysis identified 165 genes that were differentially regulated; 145 of these genes were upregulated, whereas 20 genes were downregulated. We found that several collagen genes were significantly upregulated, and a regulatory matrix metalloproteinase (MMP), MMP-3, was downregulated. Bioinformatic analysis revealed that several known signaling pathways were dysregulated, such as the TGF-β1 and Wnt signaling pathways. We also found several novel signaling pathways (e.g., PI3K, MAPK, JAK-STAT, and Notch) differentially regulated in trigger finger. The outcome of our study helps in understanding the molecular signaling pathway involved in the pathogenesis of the trigger finger.Ravindra KolheUmar GhilzaiAshis K. MondalChetan PundkarPankaj AhluwaliaNikhil S. SahajpalJie ChenCarlos M. IsalesMark FulcherSadanand FulzeleMDPI AGarticletrigger fingerpaingene expressionNanostringMedicineRENHealthcare, Vol 9, Iss 1592, p 1592 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	trigger finger pain gene expression Nanostring Medicine R
spellingShingle	trigger finger pain gene expression Nanostring Medicine R Ravindra Kolhe Umar Ghilzai Ashis K. Mondal Chetan Pundkar Pankaj Ahluwalia Nikhil S. Sahajpal Jie Chen Carlos M. Isales Mark Fulcher Sadanand Fulzele Nanostring-Based Identification of the Gene Expression Profile in Trigger Finger Samples
description	Trigger finger is a common yet vastly understudied fibroproliferative hand pathology, severely affecting patients’ quality of life. Consistent trauma due to inadequate positioning within the afflicted finger’s tendon/pulley system leads to cellular dysregulation and eventual fibrosis. While the genetic characteristics of the fibrotic tissue in the trigger finger have been studied, the pathways that govern the initiation and propagation of fibrosis are still unknown. The complete gene expression profile of the trigger finger has never been explored. Our study has used the Nanostring nCounter gene expression assay to investigate the molecular signaling involved in trigger finger pathogenesis. We collected samples from patients undergoing trigger finger (<i>n</i> = 4) release surgery and compared the gene expression to carpal tunnel tissue (<i>n</i> = 4). Nanostring nCounter analysis identified 165 genes that were differentially regulated; 145 of these genes were upregulated, whereas 20 genes were downregulated. We found that several collagen genes were significantly upregulated, and a regulatory matrix metalloproteinase (MMP), MMP-3, was downregulated. Bioinformatic analysis revealed that several known signaling pathways were dysregulated, such as the TGF-β1 and Wnt signaling pathways. We also found several novel signaling pathways (e.g., PI3K, MAPK, JAK-STAT, and Notch) differentially regulated in trigger finger. The outcome of our study helps in understanding the molecular signaling pathway involved in the pathogenesis of the trigger finger.
format	article
author	Ravindra Kolhe Umar Ghilzai Ashis K. Mondal Chetan Pundkar Pankaj Ahluwalia Nikhil S. Sahajpal Jie Chen Carlos M. Isales Mark Fulcher Sadanand Fulzele
author_facet	Ravindra Kolhe Umar Ghilzai Ashis K. Mondal Chetan Pundkar Pankaj Ahluwalia Nikhil S. Sahajpal Jie Chen Carlos M. Isales Mark Fulcher Sadanand Fulzele
author_sort	Ravindra Kolhe
title	Nanostring-Based Identification of the Gene Expression Profile in Trigger Finger Samples
title_short	Nanostring-Based Identification of the Gene Expression Profile in Trigger Finger Samples
title_full	Nanostring-Based Identification of the Gene Expression Profile in Trigger Finger Samples
title_fullStr	Nanostring-Based Identification of the Gene Expression Profile in Trigger Finger Samples
title_full_unstemmed	Nanostring-Based Identification of the Gene Expression Profile in Trigger Finger Samples
title_sort	nanostring-based identification of the gene expression profile in trigger finger samples
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/24515fc1811c4fd488a6ea56a11c1370
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Nanostring-Based Identification of the Gene Expression Profile in Trigger Finger Samples

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