Genetic characteristics of Bursaphelenchus xylophilus third-stage dispersal juveniles

Abstract The third-stage dispersal juvenile (DJ3) of pinewood nematode (PWN) is highly associated with low-temperature survival and spread of the nematode. Oil-Red-O staining showed that its lipid content was significantly higher compared with other PWN stages. Weighted gene coexpression network ana...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Qiaoli Chen, Ruizhi Zhang, Danlei Li, Feng Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/24556f4d4df64b01bd69beab508e2dd7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:24556f4d4df64b01bd69beab508e2dd7
record_format	dspace
spelling	oai:doaj.org-article:24556f4d4df64b01bd69beab508e2dd72021-12-02T10:54:06ZGenetic characteristics of Bursaphelenchus xylophilus third-stage dispersal juveniles10.1038/s41598-021-82343-92045-2322https://doaj.org/article/24556f4d4df64b01bd69beab508e2dd72021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82343-9https://doaj.org/toc/2045-2322Abstract The third-stage dispersal juvenile (DJ3) of pinewood nematode (PWN) is highly associated with low-temperature survival and spread of the nematode. Oil-Red-O staining showed that its lipid content was significantly higher compared with other PWN stages. Weighted gene coexpression network analysis identified that genes in the pink module were highly related to DJ3 induced in the laboratory (DJ3-lab). These genes were arranged according to their gene significance (GS) to DJ3-lab. Of the top 30 genes with the highest GS, seven were found to be highly homologous to the cysteine protease family cathepsin 1 (CATH1). The top 30 genes with the highest weight value to each of the seven genes in the pink module were selected, and finally 35 genes were obtained. Between these seven CATH1 homologous genes and their 35 highly related genes, 15 were related to fat metabolism or autophagy. These autophagy-related genes were also found to be highly correlated with other genes in the pink module, suggesting that autophagy might be involved in the mechanism of longevity in DJ3 and the formation of DJ3 by regulating genes related to fat metabolism.Qiaoli ChenRuizhi ZhangDanlei LiFeng WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qiaoli Chen Ruizhi Zhang Danlei Li Feng Wang Genetic characteristics of Bursaphelenchus xylophilus third-stage dispersal juveniles
description	Abstract The third-stage dispersal juvenile (DJ3) of pinewood nematode (PWN) is highly associated with low-temperature survival and spread of the nematode. Oil-Red-O staining showed that its lipid content was significantly higher compared with other PWN stages. Weighted gene coexpression network analysis identified that genes in the pink module were highly related to DJ3 induced in the laboratory (DJ3-lab). These genes were arranged according to their gene significance (GS) to DJ3-lab. Of the top 30 genes with the highest GS, seven were found to be highly homologous to the cysteine protease family cathepsin 1 (CATH1). The top 30 genes with the highest weight value to each of the seven genes in the pink module were selected, and finally 35 genes were obtained. Between these seven CATH1 homologous genes and their 35 highly related genes, 15 were related to fat metabolism or autophagy. These autophagy-related genes were also found to be highly correlated with other genes in the pink module, suggesting that autophagy might be involved in the mechanism of longevity in DJ3 and the formation of DJ3 by regulating genes related to fat metabolism.
format	article
author	Qiaoli Chen Ruizhi Zhang Danlei Li Feng Wang
author_facet	Qiaoli Chen Ruizhi Zhang Danlei Li Feng Wang
author_sort	Qiaoli Chen
title	Genetic characteristics of Bursaphelenchus xylophilus third-stage dispersal juveniles
title_short	Genetic characteristics of Bursaphelenchus xylophilus third-stage dispersal juveniles
title_full	Genetic characteristics of Bursaphelenchus xylophilus third-stage dispersal juveniles
title_fullStr	Genetic characteristics of Bursaphelenchus xylophilus third-stage dispersal juveniles
title_full_unstemmed	Genetic characteristics of Bursaphelenchus xylophilus third-stage dispersal juveniles
title_sort	genetic characteristics of bursaphelenchus xylophilus third-stage dispersal juveniles
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/24556f4d4df64b01bd69beab508e2dd7
work_keys_str_mv	AT qiaolichen geneticcharacteristicsofbursaphelenchusxylophilusthirdstagedispersaljuveniles AT ruizhizhang geneticcharacteristicsofbursaphelenchusxylophilusthirdstagedispersaljuveniles AT danleili geneticcharacteristicsofbursaphelenchusxylophilusthirdstagedispersaljuveniles AT fengwang geneticcharacteristicsofbursaphelenchusxylophilusthirdstagedispersaljuveniles
_version_	1718396457887727616

Genetic characteristics of Bursaphelenchus xylophilus third-stage dispersal juveniles

Ejemplares similares