Acetylation of Chromatin-Associated Histone H3 Lysine 56 Inhibits the Development of Encysted Artemia Embryos.

<h4>Background</h4>As a response to harsh environments, the crustacean artemia produces diapause gastrula embryos (cysts), in which cell division and embryonic development are totally arrested. This dormant state can last for very long periods but be terminated by specific environmental...

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Autores principales:	Rong Zhou, Fan Yang, Dian-Fu Chen, Yu-Xia Sun, Jin-Shu Yang, Wei-Jun Yang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/24559c3fb12046edbc7a8273f851ad26
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spelling	oai:doaj.org-article:24559c3fb12046edbc7a8273f851ad262021-11-18T07:40:59ZAcetylation of Chromatin-Associated Histone H3 Lysine 56 Inhibits the Development of Encysted Artemia Embryos.1932-620310.1371/journal.pone.0068374https://doaj.org/article/24559c3fb12046edbc7a8273f851ad262013-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0068374https://doaj.org/toc/1932-6203<h4>Background</h4>As a response to harsh environments, the crustacean artemia produces diapause gastrula embryos (cysts), in which cell division and embryonic development are totally arrested. This dormant state can last for very long periods but be terminated by specific environmental stimuli. Thus, artemia is an ideal model organism in which to study cell cycle arrest and embryonic development.<h4>Principal finding</h4>Our study focuses on the roles of H3K56ac in the arrest of cell cycle and development during artemia diapause formation and termination. We found that the level of H3K56ac on chromatin increased during diapause formation, and decreased upon diapause termination, remaining basal level throughout subsequent embryonic development. In both HeLa cells and artemia, blocking the deacetylation with nicotinamide, a histone deacetylase inhibitor, increased the level of H3K56ac on chromatin and induced an artificial cell cycle arrest. Furthermore, we found that this arrest of the cell cycle and development was induced by H3K56ac and dephosphorylation of the checkpoint protein, retinoblastoma protein.<h4>Conclusions/significance</h4>These results have revealed the dynamic change in H3K56ac on chromatin during artemia diapause formation and termination. Thus, our findings provide insight into the regulation of cell division during arrest of artemia embryonic development and provide further insight into the functions of H3K56ac.Rong ZhouFan YangDian-Fu ChenYu-Xia SunJin-Shu YangWei-Jun YangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e68374 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rong Zhou Fan Yang Dian-Fu Chen Yu-Xia Sun Jin-Shu Yang Wei-Jun Yang Acetylation of Chromatin-Associated Histone H3 Lysine 56 Inhibits the Development of Encysted Artemia Embryos.
description	<h4>Background</h4>As a response to harsh environments, the crustacean artemia produces diapause gastrula embryos (cysts), in which cell division and embryonic development are totally arrested. This dormant state can last for very long periods but be terminated by specific environmental stimuli. Thus, artemia is an ideal model organism in which to study cell cycle arrest and embryonic development.<h4>Principal finding</h4>Our study focuses on the roles of H3K56ac in the arrest of cell cycle and development during artemia diapause formation and termination. We found that the level of H3K56ac on chromatin increased during diapause formation, and decreased upon diapause termination, remaining basal level throughout subsequent embryonic development. In both HeLa cells and artemia, blocking the deacetylation with nicotinamide, a histone deacetylase inhibitor, increased the level of H3K56ac on chromatin and induced an artificial cell cycle arrest. Furthermore, we found that this arrest of the cell cycle and development was induced by H3K56ac and dephosphorylation of the checkpoint protein, retinoblastoma protein.<h4>Conclusions/significance</h4>These results have revealed the dynamic change in H3K56ac on chromatin during artemia diapause formation and termination. Thus, our findings provide insight into the regulation of cell division during arrest of artemia embryonic development and provide further insight into the functions of H3K56ac.
format	article
author	Rong Zhou Fan Yang Dian-Fu Chen Yu-Xia Sun Jin-Shu Yang Wei-Jun Yang
author_facet	Rong Zhou Fan Yang Dian-Fu Chen Yu-Xia Sun Jin-Shu Yang Wei-Jun Yang
author_sort	Rong Zhou
title	Acetylation of Chromatin-Associated Histone H3 Lysine 56 Inhibits the Development of Encysted Artemia Embryos.
title_short	Acetylation of Chromatin-Associated Histone H3 Lysine 56 Inhibits the Development of Encysted Artemia Embryos.
title_full	Acetylation of Chromatin-Associated Histone H3 Lysine 56 Inhibits the Development of Encysted Artemia Embryos.
title_fullStr	Acetylation of Chromatin-Associated Histone H3 Lysine 56 Inhibits the Development of Encysted Artemia Embryos.
title_full_unstemmed	Acetylation of Chromatin-Associated Histone H3 Lysine 56 Inhibits the Development of Encysted Artemia Embryos.
title_sort	acetylation of chromatin-associated histone h3 lysine 56 inhibits the development of encysted artemia embryos.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/24559c3fb12046edbc7a8273f851ad26
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Acetylation of Chromatin-Associated Histone H3 Lysine 56 Inhibits the Development of Encysted Artemia Embryos.

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