Heat stress elicits remodeling in the anther lipidome of peanut

Abstract Understanding the changes in peanut (Arachis hypogaea L.) anther lipidome under heat stress (HT) will aid in understanding the mechanisms of heat tolerance. We profiled the anther lipidome of seven genotypes exposed to ambient temperature (AT) or HT during flowering. Under AT and HT, the li...

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Autores principales:	Zolian S. Zoong Lwe, Ruth Welti, Daniel Anco, Salman Naveed, Sachin Rustgi, Sruthi Narayanan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2458557076df4647aacea94452194d6c
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spelling	oai:doaj.org-article:2458557076df4647aacea94452194d6c2021-12-02T12:42:28ZHeat stress elicits remodeling in the anther lipidome of peanut10.1038/s41598-020-78695-32045-2322https://doaj.org/article/2458557076df4647aacea94452194d6c2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78695-3https://doaj.org/toc/2045-2322Abstract Understanding the changes in peanut (Arachis hypogaea L.) anther lipidome under heat stress (HT) will aid in understanding the mechanisms of heat tolerance. We profiled the anther lipidome of seven genotypes exposed to ambient temperature (AT) or HT during flowering. Under AT and HT, the lipidome was dominated by phosphatidylcholine (PC), phosphatidylethanolamine (PE), and triacylglycerol (TAG) species (> 50% of total lipids). Of 89 lipid analytes specified by total acyl carbons:total carbon–carbon double bonds, 36:6, 36:5, and 34:3 PC and 34:3 PE (all contain 18:3 fatty acid and decreased under HT) were the most important lipids that differentiated HT from AT. Heat stress caused decreases in unsaturation indices of membrane lipids, primarily due to decreases in highly-unsaturated lipid species that contained 18:3 fatty acids. In parallel, the expression of Fatty Acid Desaturase 3-2 (FAD3-2; converts 18:2 fatty acids to 18:3) decreased under HT for the heat-tolerant genotype SPT 06-07 but not for the susceptible genotype Bailey. Our results suggested that decreasing lipid unsaturation levels by lowering 18:3 fatty-acid amount through reducing FAD3 expression is likely an acclimation mechanism to heat stress in peanut. Thus, genotypes that are more efficient in doing so will be relatively more tolerant to HT.Zolian S. Zoong LweRuth WeltiDaniel AncoSalman NaveedSachin RustgiSruthi NarayananNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-18 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zolian S. Zoong Lwe Ruth Welti Daniel Anco Salman Naveed Sachin Rustgi Sruthi Narayanan Heat stress elicits remodeling in the anther lipidome of peanut
description	Abstract Understanding the changes in peanut (Arachis hypogaea L.) anther lipidome under heat stress (HT) will aid in understanding the mechanisms of heat tolerance. We profiled the anther lipidome of seven genotypes exposed to ambient temperature (AT) or HT during flowering. Under AT and HT, the lipidome was dominated by phosphatidylcholine (PC), phosphatidylethanolamine (PE), and triacylglycerol (TAG) species (> 50% of total lipids). Of 89 lipid analytes specified by total acyl carbons:total carbon–carbon double bonds, 36:6, 36:5, and 34:3 PC and 34:3 PE (all contain 18:3 fatty acid and decreased under HT) were the most important lipids that differentiated HT from AT. Heat stress caused decreases in unsaturation indices of membrane lipids, primarily due to decreases in highly-unsaturated lipid species that contained 18:3 fatty acids. In parallel, the expression of Fatty Acid Desaturase 3-2 (FAD3-2; converts 18:2 fatty acids to 18:3) decreased under HT for the heat-tolerant genotype SPT 06-07 but not for the susceptible genotype Bailey. Our results suggested that decreasing lipid unsaturation levels by lowering 18:3 fatty-acid amount through reducing FAD3 expression is likely an acclimation mechanism to heat stress in peanut. Thus, genotypes that are more efficient in doing so will be relatively more tolerant to HT.
format	article
author	Zolian S. Zoong Lwe Ruth Welti Daniel Anco Salman Naveed Sachin Rustgi Sruthi Narayanan
author_facet	Zolian S. Zoong Lwe Ruth Welti Daniel Anco Salman Naveed Sachin Rustgi Sruthi Narayanan
author_sort	Zolian S. Zoong Lwe
title	Heat stress elicits remodeling in the anther lipidome of peanut
title_short	Heat stress elicits remodeling in the anther lipidome of peanut
title_full	Heat stress elicits remodeling in the anther lipidome of peanut
title_fullStr	Heat stress elicits remodeling in the anther lipidome of peanut
title_full_unstemmed	Heat stress elicits remodeling in the anther lipidome of peanut
title_sort	heat stress elicits remodeling in the anther lipidome of peanut
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/2458557076df4647aacea94452194d6c
work_keys_str_mv	AT zolianszoonglwe heatstresselicitsremodelingintheantherlipidomeofpeanut AT ruthwelti heatstresselicitsremodelingintheantherlipidomeofpeanut AT danielanco heatstresselicitsremodelingintheantherlipidomeofpeanut AT salmannaveed heatstresselicitsremodelingintheantherlipidomeofpeanut AT sachinrustgi heatstresselicitsremodelingintheantherlipidomeofpeanut AT sruthinarayanan heatstresselicitsremodelingintheantherlipidomeofpeanut
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Heat stress elicits remodeling in the anther lipidome of peanut

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