Linkers for oligonucleotide microarray synthesis

A number of linkers for microchip-based (microarray) synthesis of oligonucleotides were synthesized here using photolabile intermediates. The resulting linkers are designed for covalent immobilization on slides containing both hydroxyl and amino groups.The first series of linkers was intended for th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	A.N. Sinyakov, V.A. Ryabinin, E.V. Kostina, D.E. Zaytsev, N.V. Chukanov, G.N. Kamaev
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Synthesis Oligonucleotide Hybridization analysis Assembly of an artificial genes DNA Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/2463728a020241e5b1786dc87d91f3de
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2463728a020241e5b1786dc87d91f3de
record_format	dspace
spelling	oai:doaj.org-article:2463728a020241e5b1786dc87d91f3de2021-11-18T04:45:55ZLinkers for oligonucleotide microarray synthesis1319-610310.1016/j.jscs.2021.101382https://doaj.org/article/2463728a020241e5b1786dc87d91f3de2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319610321001873https://doaj.org/toc/1319-6103A number of linkers for microchip-based (microarray) synthesis of oligonucleotides were synthesized here using photolabile intermediates. The resulting linkers are designed for covalent immobilization on slides containing both hydroxyl and amino groups.The first series of linkers was intended for the synthesis of oligonucleotide arrays for hybridization analysis. These linkers provide a strong covalent bond with the slide surface when amino groups in oligonucleotide heterocyclic bases are deprotected.The second series of linkers allows to cleave the synthesized oligonucleotides from the support on which they were synthesized. Furthermore, the use of such linkers yields oligonucleotides devoid of the phosphate group at their 3′ end.The obtained linkers were successfully tested in the synthesis of oligonucleotides on a microchip for subsequent hybridization analysis and assembly of a DNA fragment.A.N. SinyakovV.A. RyabininE.V. KostinaD.E. ZaytsevN.V. ChukanovG.N. KamaevElsevierarticleSynthesisOligonucleotideHybridization analysisAssembly of an artificial genesDNAChemistryQD1-999ENJournal of Saudi Chemical Society, Vol 25, Iss 12, Pp 101382- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Synthesis Oligonucleotide Hybridization analysis Assembly of an artificial genes DNA Chemistry QD1-999
spellingShingle	Synthesis Oligonucleotide Hybridization analysis Assembly of an artificial genes DNA Chemistry QD1-999 A.N. Sinyakov V.A. Ryabinin E.V. Kostina D.E. Zaytsev N.V. Chukanov G.N. Kamaev Linkers for oligonucleotide microarray synthesis
description	A number of linkers for microchip-based (microarray) synthesis of oligonucleotides were synthesized here using photolabile intermediates. The resulting linkers are designed for covalent immobilization on slides containing both hydroxyl and amino groups.The first series of linkers was intended for the synthesis of oligonucleotide arrays for hybridization analysis. These linkers provide a strong covalent bond with the slide surface when amino groups in oligonucleotide heterocyclic bases are deprotected.The second series of linkers allows to cleave the synthesized oligonucleotides from the support on which they were synthesized. Furthermore, the use of such linkers yields oligonucleotides devoid of the phosphate group at their 3′ end.The obtained linkers were successfully tested in the synthesis of oligonucleotides on a microchip for subsequent hybridization analysis and assembly of a DNA fragment.
format	article
author	A.N. Sinyakov V.A. Ryabinin E.V. Kostina D.E. Zaytsev N.V. Chukanov G.N. Kamaev
author_facet	A.N. Sinyakov V.A. Ryabinin E.V. Kostina D.E. Zaytsev N.V. Chukanov G.N. Kamaev
author_sort	A.N. Sinyakov
title	Linkers for oligonucleotide microarray synthesis
title_short	Linkers for oligonucleotide microarray synthesis
title_full	Linkers for oligonucleotide microarray synthesis
title_fullStr	Linkers for oligonucleotide microarray synthesis
title_full_unstemmed	Linkers for oligonucleotide microarray synthesis
title_sort	linkers for oligonucleotide microarray synthesis
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/2463728a020241e5b1786dc87d91f3de
work_keys_str_mv	AT ansinyakov linkersforoligonucleotidemicroarraysynthesis AT varyabinin linkersforoligonucleotidemicroarraysynthesis AT evkostina linkersforoligonucleotidemicroarraysynthesis AT dezaytsev linkersforoligonucleotidemicroarraysynthesis AT nvchukanov linkersforoligonucleotidemicroarraysynthesis AT gnkamaev linkersforoligonucleotidemicroarraysynthesis
_version_	1718425026699460608

Linkers for oligonucleotide microarray synthesis

Ejemplares similares