Inactivation and sensitization of Pseudomonas aeruginosa by microplasma jet array for treating otitis media

Abstract Otitis media (OM), known as a middle ear infection, is the leading cause of antibiotic prescriptions for children. With wide-spread use of antibiotics in OM, resistance to antibiotics continues to decrease the efficacy of the treatment. Furthermore, as the presence of a middle ear biofilm h...

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Autores principales:	Peter P. Sun, Jungeun Won, Gabrielle Choo-Kang, Shouyan Li, Wenyuan Chen, Guillermo L. Monroy, Eric J. Chaney, Stephen A. Boppart, J. Gary Eden, Thanh H. Nguyen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/246c0750729e4576bbadf6c475420f6a
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spelling	oai:doaj.org-article:246c0750729e4576bbadf6c475420f6a2021-12-02T15:02:24ZInactivation and sensitization of Pseudomonas aeruginosa by microplasma jet array for treating otitis media10.1038/s41522-021-00219-22055-5008https://doaj.org/article/246c0750729e4576bbadf6c475420f6a2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00219-2https://doaj.org/toc/2055-5008Abstract Otitis media (OM), known as a middle ear infection, is the leading cause of antibiotic prescriptions for children. With wide-spread use of antibiotics in OM, resistance to antibiotics continues to decrease the efficacy of the treatment. Furthermore, as the presence of a middle ear biofilm has contributed to this reduced susceptibility to antimicrobials, effective interventions are necessary. A miniaturized 3D-printed microplasma jet array has been developed to inactivate Pseudomonas aeruginosa, a common bacterial strain associated with OM. The experiments demonstrate the disruption of planktonic and biofilm P. aeruginosa by long-lived molecular species generated by microplasma, as well as the synergy of combining microplasma treatment with antibiotic therapy. In addition, a middle ear phantom model was developed with an excised rat eardrum to investigate the antimicrobial effects of microplasma on bacteria located behind the eardrum, as in a patient-relevant setup. These results suggest the potential for microplasma as a new treatment paradigm for OM.Peter P. SunJungeun WonGabrielle Choo-KangShouyan LiWenyuan ChenGuillermo L. MonroyEric J. ChaneyStephen A. BoppartJ. Gary EdenThanh H. NguyenNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Peter P. Sun Jungeun Won Gabrielle Choo-Kang Shouyan Li Wenyuan Chen Guillermo L. Monroy Eric J. Chaney Stephen A. Boppart J. Gary Eden Thanh H. Nguyen Inactivation and sensitization of Pseudomonas aeruginosa by microplasma jet array for treating otitis media
description	Abstract Otitis media (OM), known as a middle ear infection, is the leading cause of antibiotic prescriptions for children. With wide-spread use of antibiotics in OM, resistance to antibiotics continues to decrease the efficacy of the treatment. Furthermore, as the presence of a middle ear biofilm has contributed to this reduced susceptibility to antimicrobials, effective interventions are necessary. A miniaturized 3D-printed microplasma jet array has been developed to inactivate Pseudomonas aeruginosa, a common bacterial strain associated with OM. The experiments demonstrate the disruption of planktonic and biofilm P. aeruginosa by long-lived molecular species generated by microplasma, as well as the synergy of combining microplasma treatment with antibiotic therapy. In addition, a middle ear phantom model was developed with an excised rat eardrum to investigate the antimicrobial effects of microplasma on bacteria located behind the eardrum, as in a patient-relevant setup. These results suggest the potential for microplasma as a new treatment paradigm for OM.
format	article
author	Peter P. Sun Jungeun Won Gabrielle Choo-Kang Shouyan Li Wenyuan Chen Guillermo L. Monroy Eric J. Chaney Stephen A. Boppart J. Gary Eden Thanh H. Nguyen
author_facet	Peter P. Sun Jungeun Won Gabrielle Choo-Kang Shouyan Li Wenyuan Chen Guillermo L. Monroy Eric J. Chaney Stephen A. Boppart J. Gary Eden Thanh H. Nguyen
author_sort	Peter P. Sun
title	Inactivation and sensitization of Pseudomonas aeruginosa by microplasma jet array for treating otitis media
title_short	Inactivation and sensitization of Pseudomonas aeruginosa by microplasma jet array for treating otitis media
title_full	Inactivation and sensitization of Pseudomonas aeruginosa by microplasma jet array for treating otitis media
title_fullStr	Inactivation and sensitization of Pseudomonas aeruginosa by microplasma jet array for treating otitis media
title_full_unstemmed	Inactivation and sensitization of Pseudomonas aeruginosa by microplasma jet array for treating otitis media
title_sort	inactivation and sensitization of pseudomonas aeruginosa by microplasma jet array for treating otitis media
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/246c0750729e4576bbadf6c475420f6a
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Inactivation and sensitization of Pseudomonas aeruginosa by microplasma jet array for treating otitis media

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