BolA Is a Transcriptional Switch That Turns Off Motility and Turns On Biofilm Development

ABSTRACT Bacteria are extremely versatile organisms that rapidly adapt to changing environments. When bacterial cells switch from planktonic growth to biofilm, flagellum formation is turned off and the production of fimbriae and extracellular polysaccharides is switched on. BolA is present in most G...

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Autores principales:	Clémentine Dressaire, Ricardo Neves Moreira, Susana Barahona, António Pedro Alves de Matos, Cecília Maria Arraiano
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2015
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/2473aa4270fd4f568a2c813191caccc9
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spelling	oai:doaj.org-article:2473aa4270fd4f568a2c813191caccc92021-11-15T15:41:19ZBolA Is a Transcriptional Switch That Turns Off Motility and Turns On Biofilm Development10.1128/mBio.02352-142150-7511https://doaj.org/article/2473aa4270fd4f568a2c813191caccc92015-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02352-14https://doaj.org/toc/2150-7511ABSTRACT Bacteria are extremely versatile organisms that rapidly adapt to changing environments. When bacterial cells switch from planktonic growth to biofilm, flagellum formation is turned off and the production of fimbriae and extracellular polysaccharides is switched on. BolA is present in most Gram-negative bacteria, and homologues can be found from proteobacteria to eukaryotes. Here, we show that BolA is a new bacterial transcription factor that modulates the switch from a planktonic to a sessile lifestyle. It negatively modulates flagellar biosynthesis and swimming capacity in Escherichia coli. Furthermore, BolA overexpression favors biofilm formation, involving the production of fimbria-like adhesins and curli. Our results also demonstrate that BolA is a protein with high affinity to DNA and is able to regulate many genes on a genome-wide scale. Moreover, we show that the most significant targets of this protein involve a complex network of genes encoding proteins related to biofilm development. Herein, we propose that BolA is a motile/adhesive transcriptional switch, specifically involved in the transition between the planktonic and the attachment stage of biofilm formation. IMPORTANCE Escherichia coli cells possess several mechanisms to cope with stresses. BolA has been described as a protein important for survival in late stages of bacterial growth and under harsh environmental conditions. BolA-like proteins are widely conserved from prokaryotes to eukaryotes. Although their exact function is not fully established at the molecular level, they seem to be involved in cell proliferation or cell cycle regulation. Here, we unraveled the role of BolA in biofilm development and bacterial motility. Our work suggests that BolA actively contributes to the decision of bacteria to arrest flagellar production and initiate the attachment to form structured communities, such as biofilms. The molecular studies of different lifestyles coupled with the comprehension of the BolA functions may be an important step for future perspectives, with health care and biotechnology applications.Clémentine DressaireRicardo Neves MoreiraSusana BarahonaAntónio Pedro Alves de MatosCecília Maria ArraianoAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 6, Iss 1 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Clémentine Dressaire Ricardo Neves Moreira Susana Barahona António Pedro Alves de Matos Cecília Maria Arraiano BolA Is a Transcriptional Switch That Turns Off Motility and Turns On Biofilm Development
description	ABSTRACT Bacteria are extremely versatile organisms that rapidly adapt to changing environments. When bacterial cells switch from planktonic growth to biofilm, flagellum formation is turned off and the production of fimbriae and extracellular polysaccharides is switched on. BolA is present in most Gram-negative bacteria, and homologues can be found from proteobacteria to eukaryotes. Here, we show that BolA is a new bacterial transcription factor that modulates the switch from a planktonic to a sessile lifestyle. It negatively modulates flagellar biosynthesis and swimming capacity in Escherichia coli. Furthermore, BolA overexpression favors biofilm formation, involving the production of fimbria-like adhesins and curli. Our results also demonstrate that BolA is a protein with high affinity to DNA and is able to regulate many genes on a genome-wide scale. Moreover, we show that the most significant targets of this protein involve a complex network of genes encoding proteins related to biofilm development. Herein, we propose that BolA is a motile/adhesive transcriptional switch, specifically involved in the transition between the planktonic and the attachment stage of biofilm formation. IMPORTANCE Escherichia coli cells possess several mechanisms to cope with stresses. BolA has been described as a protein important for survival in late stages of bacterial growth and under harsh environmental conditions. BolA-like proteins are widely conserved from prokaryotes to eukaryotes. Although their exact function is not fully established at the molecular level, they seem to be involved in cell proliferation or cell cycle regulation. Here, we unraveled the role of BolA in biofilm development and bacterial motility. Our work suggests that BolA actively contributes to the decision of bacteria to arrest flagellar production and initiate the attachment to form structured communities, such as biofilms. The molecular studies of different lifestyles coupled with the comprehension of the BolA functions may be an important step for future perspectives, with health care and biotechnology applications.
format	article
author	Clémentine Dressaire Ricardo Neves Moreira Susana Barahona António Pedro Alves de Matos Cecília Maria Arraiano
author_facet	Clémentine Dressaire Ricardo Neves Moreira Susana Barahona António Pedro Alves de Matos Cecília Maria Arraiano
author_sort	Clémentine Dressaire
title	BolA Is a Transcriptional Switch That Turns Off Motility and Turns On Biofilm Development
title_short	BolA Is a Transcriptional Switch That Turns Off Motility and Turns On Biofilm Development
title_full	BolA Is a Transcriptional Switch That Turns Off Motility and Turns On Biofilm Development
title_fullStr	BolA Is a Transcriptional Switch That Turns Off Motility and Turns On Biofilm Development
title_full_unstemmed	BolA Is a Transcriptional Switch That Turns Off Motility and Turns On Biofilm Development
title_sort	bola is a transcriptional switch that turns off motility and turns on biofilm development
publisher	American Society for Microbiology
publishDate	2015
url	https://doaj.org/article/2473aa4270fd4f568a2c813191caccc9
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BolA Is a Transcriptional Switch That Turns Off Motility and Turns On Biofilm Development

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