Whole transcriptome analysis of high and low IFN‐α producers reveals differential response patterns following rhinovirus stimulation

Abstract Objectives Viral respiratory infections cause considerable morbidity and economic loss. While rhinoviruses (RV) typically cause little more than the common cold, they can produce severe infections and disease exacerbations in susceptible individuals, such as those with asthma. Variations in...

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Autores principales:	Liisa M Murray, Gayathri Thillaiyampalam, Yang Xi, Alexandre S Cristino, John W Upham
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	antiviral immune response asthma gene expression IFN‐α immune variability interferon Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/247612ac82fc44dfa7a1978c4533959a
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spelling	oai:doaj.org-article:247612ac82fc44dfa7a1978c4533959a2021-11-25T13:32:31ZWhole transcriptome analysis of high and low IFN‐α producers reveals differential response patterns following rhinovirus stimulation2050-006810.1002/cti2.1356https://doaj.org/article/247612ac82fc44dfa7a1978c4533959a2021-01-01T00:00:00Zhttps://doi.org/10.1002/cti2.1356https://doaj.org/toc/2050-0068Abstract Objectives Viral respiratory infections cause considerable morbidity and economic loss. While rhinoviruses (RV) typically cause little more than the common cold, they can produce severe infections and disease exacerbations in susceptible individuals, such as those with asthma. Variations in the regulation of key antiviral cytokines, particularly type I interferon (IFN‐α and IFN‐β), may contribute to RV susceptibility. To understand this variability, we compared the transcriptomes of high and low type I IFN producers. Methods Blood mononuclear cells from 238 individuals with or without asthma were cultured in the presence or absence of RV. Those samples demonstrating high or low RV‐stimulated IFN‐α production (N = 75) underwent RNA‐sequencing. Results Gene expression patterns were similar in samples from healthy participants and those with asthma. At baseline, the high IFN‐α producer group showed higher expression of genes associated with plasmacytoid dendritic cells, the innate immune response and vitamin D activation, but lower expression of oxidative stress pathways than the low IFN‐α producer group. After RV stimulation, the high IFN‐α producer group showed higher expression of genes found in immune response biological pathways and lower expression of genes linked to developmental and catabolic processes when compared to the low IFN‐α producer group. Conclusions These differences suggest that the high IFN‐α group has a higher level of immune system readiness, resulting in a more intense and perhaps more focussed pathogen‐specific immune response. These results contribute to a better understanding of the variability in type I IFN production between individuals.Liisa M MurrayGayathri ThillaiyampalamYang XiAlexandre S CristinoJohn W UphamWileyarticleantiviral immune responseasthmagene expressionIFN‐αimmune variabilityinterferonImmunologic diseases. AllergyRC581-607ENClinical & Translational Immunology, Vol 10, Iss 11, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antiviral immune response asthma gene expression IFN‐α immune variability interferon Immunologic diseases. Allergy RC581-607
spellingShingle	antiviral immune response asthma gene expression IFN‐α immune variability interferon Immunologic diseases. Allergy RC581-607 Liisa M Murray Gayathri Thillaiyampalam Yang Xi Alexandre S Cristino John W Upham Whole transcriptome analysis of high and low IFN‐α producers reveals differential response patterns following rhinovirus stimulation
description	Abstract Objectives Viral respiratory infections cause considerable morbidity and economic loss. While rhinoviruses (RV) typically cause little more than the common cold, they can produce severe infections and disease exacerbations in susceptible individuals, such as those with asthma. Variations in the regulation of key antiviral cytokines, particularly type I interferon (IFN‐α and IFN‐β), may contribute to RV susceptibility. To understand this variability, we compared the transcriptomes of high and low type I IFN producers. Methods Blood mononuclear cells from 238 individuals with or without asthma were cultured in the presence or absence of RV. Those samples demonstrating high or low RV‐stimulated IFN‐α production (N = 75) underwent RNA‐sequencing. Results Gene expression patterns were similar in samples from healthy participants and those with asthma. At baseline, the high IFN‐α producer group showed higher expression of genes associated with plasmacytoid dendritic cells, the innate immune response and vitamin D activation, but lower expression of oxidative stress pathways than the low IFN‐α producer group. After RV stimulation, the high IFN‐α producer group showed higher expression of genes found in immune response biological pathways and lower expression of genes linked to developmental and catabolic processes when compared to the low IFN‐α producer group. Conclusions These differences suggest that the high IFN‐α group has a higher level of immune system readiness, resulting in a more intense and perhaps more focussed pathogen‐specific immune response. These results contribute to a better understanding of the variability in type I IFN production between individuals.
format	article
author	Liisa M Murray Gayathri Thillaiyampalam Yang Xi Alexandre S Cristino John W Upham
author_facet	Liisa M Murray Gayathri Thillaiyampalam Yang Xi Alexandre S Cristino John W Upham
author_sort	Liisa M Murray
title	Whole transcriptome analysis of high and low IFN‐α producers reveals differential response patterns following rhinovirus stimulation
title_short	Whole transcriptome analysis of high and low IFN‐α producers reveals differential response patterns following rhinovirus stimulation
title_full	Whole transcriptome analysis of high and low IFN‐α producers reveals differential response patterns following rhinovirus stimulation
title_fullStr	Whole transcriptome analysis of high and low IFN‐α producers reveals differential response patterns following rhinovirus stimulation
title_full_unstemmed	Whole transcriptome analysis of high and low IFN‐α producers reveals differential response patterns following rhinovirus stimulation
title_sort	whole transcriptome analysis of high and low ifn‐α producers reveals differential response patterns following rhinovirus stimulation
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/247612ac82fc44dfa7a1978c4533959a
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_version_	1718413424887595008

Whole transcriptome analysis of high and low IFN‐α producers reveals differential response patterns following rhinovirus stimulation

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