Nano-Scaled Zeolitic Imidazole Framework-8 as an Efficient Carrier for the Intracellular Delivery of RNase A in Cancer Treatment

Jiaxin Jia, Shudi Zhang, Kai Wen, Quanshun Li Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, People’s Republic of ChinaCorrespondence: Quanshun Li Tel +86-431-85155201Fax +86-431-85155200Email q...

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Autores principales:	Jia J, Zhang S, Wen K, Li Q
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	zeolitic imidazole framework-8 rnase a biomimetic mineralization protein delivery metal-organic frameworks Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/247d42ca976d4a359691e4918606f125
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spelling	oai:doaj.org-article:247d42ca976d4a359691e4918606f1252021-12-02T05:37:43ZNano-Scaled Zeolitic Imidazole Framework-8 as an Efficient Carrier for the Intracellular Delivery of RNase A in Cancer Treatment1178-2013https://doaj.org/article/247d42ca976d4a359691e4918606f1252019-12-01T00:00:00Zhttps://www.dovepress.com/nano-scaled-zeolitic-imidazole-framework-8-as-an-efficient-carrier-for-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jiaxin Jia, Shudi Zhang, Kai Wen, Quanshun Li Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, People’s Republic of ChinaCorrespondence: Quanshun Li Tel +86-431-85155201Fax +86-431-85155200Email quanshun@jlu.edu.cnBackground: Zeolitic imidazole framework-8 (ZIF-8) as an emerging platform has exhibited great potential in the protein delivery owing to its tunable chemical functionality.Materials and methods: ZIF-8 was employed as a carrier for the encapsulation and intracellular delivery of RNase A, aimed to achieve a rapid release of proteins in an acidic environment. The intracellular uptake of RNase A was studied by confocal laser scanning microscopy (CLSM), and the inhibition of cell proliferation after the delivery of RNase A was evaluated by MTT assay, Live/Dead staining, and TUNEL cell apoptosis analysis, using human lung adenocarcinoma cell line A549 as a model. The biocompatibility of RNase A@ZIF-8 nanoparticles was systematically detected through the hemolysis and cytotoxicity assay.Results: The RNase A@ZIF-8 nanoparticles constructed by biomimetic mineralization could not only facilitate the encapsulation of protein molecules (protein loading: 13.4%) but also maintain the enzymatic activity and stability of RNase A. The CLSM images showed that RNase A@ZIF-8 nanoparticles could efficiently improve the intracellular uptake of RNase A. Moreover, RNase A@ZIF-8 nanoparticles could obviously inhibit the cell proliferation through the induction of cell apoptosis, with 31.3% of cell death at an RNase A concentration of 10 μg/mL. Finally, RNase A@ZIF-8 nanoparticles were elucidated to possess excellent biocompatibility, with hemolysis of <5% using the same concentration of RNase A@ZIF-8.Conclusion: ZIF-8 could be used as an effective carrier to deliver the therapeutic protein RNase A into the cytosol, which will be beneficial for improving the efficacy of cancer treatment.Keywords: zeolitic imidazole framework-8, RNase A, biomimetic mineralization, protein delivery, metal-organic frameworks  Jia JZhang SWen KLi QDove Medical Pressarticlezeolitic imidazole framework-8rnase abiomimetic mineralizationprotein deliverymetal-organic frameworksMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 9971-9981 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	zeolitic imidazole framework-8 rnase a biomimetic mineralization protein delivery metal-organic frameworks Medicine (General) R5-920
spellingShingle	zeolitic imidazole framework-8 rnase a biomimetic mineralization protein delivery metal-organic frameworks Medicine (General) R5-920 Jia J Zhang S Wen K Li Q Nano-Scaled Zeolitic Imidazole Framework-8 as an Efficient Carrier for the Intracellular Delivery of RNase A in Cancer Treatment
description	Jiaxin Jia, Shudi Zhang, Kai Wen, Quanshun Li Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, People’s Republic of ChinaCorrespondence: Quanshun Li Tel +86-431-85155201Fax +86-431-85155200Email quanshun@jlu.edu.cnBackground: Zeolitic imidazole framework-8 (ZIF-8) as an emerging platform has exhibited great potential in the protein delivery owing to its tunable chemical functionality.Materials and methods: ZIF-8 was employed as a carrier for the encapsulation and intracellular delivery of RNase A, aimed to achieve a rapid release of proteins in an acidic environment. The intracellular uptake of RNase A was studied by confocal laser scanning microscopy (CLSM), and the inhibition of cell proliferation after the delivery of RNase A was evaluated by MTT assay, Live/Dead staining, and TUNEL cell apoptosis analysis, using human lung adenocarcinoma cell line A549 as a model. The biocompatibility of RNase A@ZIF-8 nanoparticles was systematically detected through the hemolysis and cytotoxicity assay.Results: The RNase A@ZIF-8 nanoparticles constructed by biomimetic mineralization could not only facilitate the encapsulation of protein molecules (protein loading: 13.4%) but also maintain the enzymatic activity and stability of RNase A. The CLSM images showed that RNase A@ZIF-8 nanoparticles could efficiently improve the intracellular uptake of RNase A. Moreover, RNase A@ZIF-8 nanoparticles could obviously inhibit the cell proliferation through the induction of cell apoptosis, with 31.3% of cell death at an RNase A concentration of 10 μg/mL. Finally, RNase A@ZIF-8 nanoparticles were elucidated to possess excellent biocompatibility, with hemolysis of <5% using the same concentration of RNase A@ZIF-8.Conclusion: ZIF-8 could be used as an effective carrier to deliver the therapeutic protein RNase A into the cytosol, which will be beneficial for improving the efficacy of cancer treatment.Keywords: zeolitic imidazole framework-8, RNase A, biomimetic mineralization, protein delivery, metal-organic frameworks
format	article
author	Jia J Zhang S Wen K Li Q
author_facet	Jia J Zhang S Wen K Li Q
author_sort	Jia J
title	Nano-Scaled Zeolitic Imidazole Framework-8 as an Efficient Carrier for the Intracellular Delivery of RNase A in Cancer Treatment
title_short	Nano-Scaled Zeolitic Imidazole Framework-8 as an Efficient Carrier for the Intracellular Delivery of RNase A in Cancer Treatment
title_full	Nano-Scaled Zeolitic Imidazole Framework-8 as an Efficient Carrier for the Intracellular Delivery of RNase A in Cancer Treatment
title_fullStr	Nano-Scaled Zeolitic Imidazole Framework-8 as an Efficient Carrier for the Intracellular Delivery of RNase A in Cancer Treatment
title_full_unstemmed	Nano-Scaled Zeolitic Imidazole Framework-8 as an Efficient Carrier for the Intracellular Delivery of RNase A in Cancer Treatment
title_sort	nano-scaled zeolitic imidazole framework-8 as an efficient carrier for the intracellular delivery of rnase a in cancer treatment
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/247d42ca976d4a359691e4918606f125
work_keys_str_mv	AT jiaj nanoscaledzeoliticimidazoleframework8asanefficientcarrierfortheintracellulardeliveryofrnaseaincancertreatment AT zhangs nanoscaledzeoliticimidazoleframework8asanefficientcarrierfortheintracellulardeliveryofrnaseaincancertreatment AT wenk nanoscaledzeoliticimidazoleframework8asanefficientcarrierfortheintracellulardeliveryofrnaseaincancertreatment AT liq nanoscaledzeoliticimidazoleframework8asanefficientcarrierfortheintracellulardeliveryofrnaseaincancertreatment
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Nano-Scaled Zeolitic Imidazole Framework-8 as an Efficient Carrier for the Intracellular Delivery of RNase A in Cancer Treatment

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