The mechanism of TiaoGanYiPi formula for treating chronic hepatitis B by network pharmacology and molecular docking verification
Abstract The Chinese herbal formula TiaoGanYiPi (TGYP) showed effective against chronic hepatitis B (CHB) caused by hepatitis B virus (HBV) infection. Hence, we aimed to clarify the mechanisms and potential targets between TGYP and CHB. The active compounds and related putative targets of TGYP, and...
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2021
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oai:doaj.org-article:247ec193b29f4beebb0d5dd673a331232021-12-02T18:03:14ZThe mechanism of TiaoGanYiPi formula for treating chronic hepatitis B by network pharmacology and molecular docking verification10.1038/s41598-021-87812-92045-2322https://doaj.org/article/247ec193b29f4beebb0d5dd673a331232021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87812-9https://doaj.org/toc/2045-2322Abstract The Chinese herbal formula TiaoGanYiPi (TGYP) showed effective against chronic hepatitis B (CHB) caused by hepatitis B virus (HBV) infection. Hence, we aimed to clarify the mechanisms and potential targets between TGYP and CHB. The active compounds and related putative targets of TGYP, and disease targets of CHB were obtained from the public databases. The key targets between TGYP and CHB were identified through the network construction and module analysis. The expression of the key targets was detected in Gene Expression Omnibus (GEO) dataset and normal hepatocyte cell line LO2. We first obtained 11 key targets which were predominantly enriched in the Cancer, Cell cycle and HBV-related pathways. And the expression of the key targets was related to HBV infection and liver inflammation verified in GSE83148 database. Furthermore, the results of real-time quantitative PCR and CCK-8 assay indicated that TGYP could regulate the expression of key targets including CCNA2, ABL1, CDK4, CDKN1A, IGFR and MAP2K1, and promote proliferation of LO2 cells. In coclusion, we identified the active compounds and key targets btween TGYP and CHB, and found that the TGYP might exhibite curative effect on CHB via promoting hepatocyte proliferation and inhibiting the liver inflammatory processes.Xu CaoXiaobin ZaoBaiquan XueHening ChenJiaxin ZhangShuo LiXiaobin LiShun ZhuRui GuoXiaoke LiYong’an YeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) |
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Medicine R Science Q Xu Cao Xiaobin Zao Baiquan Xue Hening Chen Jiaxin Zhang Shuo Li Xiaobin Li Shun Zhu Rui Guo Xiaoke Li Yong’an Ye The mechanism of TiaoGanYiPi formula for treating chronic hepatitis B by network pharmacology and molecular docking verification |
| description |
Abstract The Chinese herbal formula TiaoGanYiPi (TGYP) showed effective against chronic hepatitis B (CHB) caused by hepatitis B virus (HBV) infection. Hence, we aimed to clarify the mechanisms and potential targets between TGYP and CHB. The active compounds and related putative targets of TGYP, and disease targets of CHB were obtained from the public databases. The key targets between TGYP and CHB were identified through the network construction and module analysis. The expression of the key targets was detected in Gene Expression Omnibus (GEO) dataset and normal hepatocyte cell line LO2. We first obtained 11 key targets which were predominantly enriched in the Cancer, Cell cycle and HBV-related pathways. And the expression of the key targets was related to HBV infection and liver inflammation verified in GSE83148 database. Furthermore, the results of real-time quantitative PCR and CCK-8 assay indicated that TGYP could regulate the expression of key targets including CCNA2, ABL1, CDK4, CDKN1A, IGFR and MAP2K1, and promote proliferation of LO2 cells. In coclusion, we identified the active compounds and key targets btween TGYP and CHB, and found that the TGYP might exhibite curative effect on CHB via promoting hepatocyte proliferation and inhibiting the liver inflammatory processes. |
| format |
article |
| author |
Xu Cao Xiaobin Zao Baiquan Xue Hening Chen Jiaxin Zhang Shuo Li Xiaobin Li Shun Zhu Rui Guo Xiaoke Li Yong’an Ye |
| author_facet |
Xu Cao Xiaobin Zao Baiquan Xue Hening Chen Jiaxin Zhang Shuo Li Xiaobin Li Shun Zhu Rui Guo Xiaoke Li Yong’an Ye |
| author_sort |
Xu Cao |
| title |
The mechanism of TiaoGanYiPi formula for treating chronic hepatitis B by network pharmacology and molecular docking verification |
| title_short |
The mechanism of TiaoGanYiPi formula for treating chronic hepatitis B by network pharmacology and molecular docking verification |
| title_full |
The mechanism of TiaoGanYiPi formula for treating chronic hepatitis B by network pharmacology and molecular docking verification |
| title_fullStr |
The mechanism of TiaoGanYiPi formula for treating chronic hepatitis B by network pharmacology and molecular docking verification |
| title_full_unstemmed |
The mechanism of TiaoGanYiPi formula for treating chronic hepatitis B by network pharmacology and molecular docking verification |
| title_sort |
mechanism of tiaoganyipi formula for treating chronic hepatitis b by network pharmacology and molecular docking verification |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/247ec193b29f4beebb0d5dd673a33123 |
| work_keys_str_mv |
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