Interactions of an Emerging Fungal Pathogen Scedosporium aurantiacum with Human Lung Epithelial Cells

Abstract Scedosporium fungi are found in various natural and host-associated environments, including the lungs of cystic fibrosis patients. However, their role in infection development remains underexplored. Here the attachment of conidia of a virulent S. aurantiacum strain WM 06.482 onto the human...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jashanpreet Kaur, Liisa Kautto, Anahit Penesyan, Wieland Meyer, Liam D. H. Elbourne, Ian T. Paulsen, Helena Nevalainen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2481f3b5cf324e4bbe0c864d75ad25d7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2481f3b5cf324e4bbe0c864d75ad25d7
record_format	dspace
spelling	oai:doaj.org-article:2481f3b5cf324e4bbe0c864d75ad25d72021-12-02T15:09:15ZInteractions of an Emerging Fungal Pathogen Scedosporium aurantiacum with Human Lung Epithelial Cells10.1038/s41598-019-41435-32045-2322https://doaj.org/article/2481f3b5cf324e4bbe0c864d75ad25d72019-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-41435-3https://doaj.org/toc/2045-2322Abstract Scedosporium fungi are found in various natural and host-associated environments, including the lungs of cystic fibrosis patients. However, their role in infection development remains underexplored. Here the attachment of conidia of a virulent S. aurantiacum strain WM 06.482 onto the human lung epithelial A549 cells in vitro was visualized using microscopy to examine the initial steps of infection. We showed that 75–80% of fungal conidia were bound to the A549 cells within four hours of co-incubation, and started to produce germ tubes. The germinating conidia seemed to invade the cells through the intercellular space, no intracellular uptake of fungal conidia by the airway epithelial cells after conidial attachment. Transcriptomic analysis of the A549 cells revealed that the up-regulated genes were mainly associated with cell repair and inflammatory processes indicating a protective response against S. aurantiacum infection. Network analysis of the differentially expressed genes showed activation of the innate immune system (NF-kB pathway) leading to the release of pro-inflammatory cytokines. We believe this is the first report showing the transcriptomic response of human alveolar epithelial cells exposed to S. aurantiacum conidia paving a way for better understanding of the mechanism of the infection process.Jashanpreet KaurLiisa KauttoAnahit PenesyanWieland MeyerLiam D. H. ElbourneIan T. PaulsenHelena NevalainenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jashanpreet Kaur Liisa Kautto Anahit Penesyan Wieland Meyer Liam D. H. Elbourne Ian T. Paulsen Helena Nevalainen Interactions of an Emerging Fungal Pathogen Scedosporium aurantiacum with Human Lung Epithelial Cells
description	Abstract Scedosporium fungi are found in various natural and host-associated environments, including the lungs of cystic fibrosis patients. However, their role in infection development remains underexplored. Here the attachment of conidia of a virulent S. aurantiacum strain WM 06.482 onto the human lung epithelial A549 cells in vitro was visualized using microscopy to examine the initial steps of infection. We showed that 75–80% of fungal conidia were bound to the A549 cells within four hours of co-incubation, and started to produce germ tubes. The germinating conidia seemed to invade the cells through the intercellular space, no intracellular uptake of fungal conidia by the airway epithelial cells after conidial attachment. Transcriptomic analysis of the A549 cells revealed that the up-regulated genes were mainly associated with cell repair and inflammatory processes indicating a protective response against S. aurantiacum infection. Network analysis of the differentially expressed genes showed activation of the innate immune system (NF-kB pathway) leading to the release of pro-inflammatory cytokines. We believe this is the first report showing the transcriptomic response of human alveolar epithelial cells exposed to S. aurantiacum conidia paving a way for better understanding of the mechanism of the infection process.
format	article
author	Jashanpreet Kaur Liisa Kautto Anahit Penesyan Wieland Meyer Liam D. H. Elbourne Ian T. Paulsen Helena Nevalainen
author_facet	Jashanpreet Kaur Liisa Kautto Anahit Penesyan Wieland Meyer Liam D. H. Elbourne Ian T. Paulsen Helena Nevalainen
author_sort	Jashanpreet Kaur
title	Interactions of an Emerging Fungal Pathogen Scedosporium aurantiacum with Human Lung Epithelial Cells
title_short	Interactions of an Emerging Fungal Pathogen Scedosporium aurantiacum with Human Lung Epithelial Cells
title_full	Interactions of an Emerging Fungal Pathogen Scedosporium aurantiacum with Human Lung Epithelial Cells
title_fullStr	Interactions of an Emerging Fungal Pathogen Scedosporium aurantiacum with Human Lung Epithelial Cells
title_full_unstemmed	Interactions of an Emerging Fungal Pathogen Scedosporium aurantiacum with Human Lung Epithelial Cells
title_sort	interactions of an emerging fungal pathogen scedosporium aurantiacum with human lung epithelial cells
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/2481f3b5cf324e4bbe0c864d75ad25d7
work_keys_str_mv	AT jashanpreetkaur interactionsofanemergingfungalpathogenscedosporiumaurantiacumwithhumanlungepithelialcells AT liisakautto interactionsofanemergingfungalpathogenscedosporiumaurantiacumwithhumanlungepithelialcells AT anahitpenesyan interactionsofanemergingfungalpathogenscedosporiumaurantiacumwithhumanlungepithelialcells AT wielandmeyer interactionsofanemergingfungalpathogenscedosporiumaurantiacumwithhumanlungepithelialcells AT liamdhelbourne interactionsofanemergingfungalpathogenscedosporiumaurantiacumwithhumanlungepithelialcells AT iantpaulsen interactionsofanemergingfungalpathogenscedosporiumaurantiacumwithhumanlungepithelialcells AT helenanevalainen interactionsofanemergingfungalpathogenscedosporiumaurantiacumwithhumanlungepithelialcells
_version_	1718387861380661248

Interactions of an Emerging Fungal Pathogen Scedosporium aurantiacum with Human Lung Epithelial Cells

Ejemplares similares