Isolation and selection of cellulolytic bacteria from rice straw for consortium of microbial fuel cell

Abstract. Khoirunnisa NS, Anwar S, Santosa DA. 2020. Isolation and selection of cellulolytic bacteria from rice straw for consortium of microbial fuel cell. Biodiversitas 21: 1686-1696. Cellulose such as in rice straw can be utilized as an organic substrate in Microbial Fuel Cell (MFC) to generate e...

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Autores principales:	Nur Syafira Khoirunnisa, SYAIFUL ANWAR, DWI ANDREAS SANTOSA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	bacteria, cellulose, decomposition, degrading, exoelectrogen Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/248c9ec7ecf54140b6590edf9af94692
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spelling	oai:doaj.org-article:248c9ec7ecf54140b6590edf9af946922021-11-22T00:34:39ZIsolation and selection of cellulolytic bacteria from rice straw for consortium of microbial fuel cell1412-033X2085-472210.13057/biodiv/d210450https://doaj.org/article/248c9ec7ecf54140b6590edf9af946922020-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5092https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Khoirunnisa NS, Anwar S, Santosa DA. 2020. Isolation and selection of cellulolytic bacteria from rice straw for consortium of microbial fuel cell. Biodiversitas 21: 1686-1696. Cellulose such as in rice straw can be utilized as an organic substrate in Microbial Fuel Cell (MFC) to generate electricity by microorganisms as a biocatalyst. This research aimed to get cellulose-degrading bacteria with high capability to degrade rice straw and able to be used as consortium with exoelectrogen bacteria in Microbial Fuel Cell. The stages of research included: (i) isolation of the bacteria using carboxymethylcellulose (CMC) agar medium, (ii) selection of the isolates for that purpose, (iii) enzyme assay and MFC performance test, and (iv) identification of selected isolate. There were 125 isolates that were obtained. Selection based on the ability to degrade cellulose as indicated by clear zone on CMC medium resulted in 23 isolates. Ten isolates belong to anaerobic facultative bacteria were selected. Three of them were synergistic with exoelectrogen bacteria. The three isolates were tested for exoglucanase (Avicel) and total enzyme activity (Filter Paper) with the highest results were 6.21 U/mL (isolate J404) and 5.88 U/mL (isolate J401), respectively. The optimum MFC performance was achieved by one isolate, J401, which produced highest voltage of 40.8 mV and a power density of 0.33 mW/m2. The best isolate, J401, was identified as Xanthomonas translucens based on 16S rRNA method.Nur Syafira KhoirunnisaSYAIFUL ANWARDWI ANDREAS SANTOSAMBI & UNS Soloarticlebacteria, cellulose, decomposition, degrading, exoelectrogenBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteria, cellulose, decomposition, degrading, exoelectrogen Biology (General) QH301-705.5
spellingShingle	bacteria, cellulose, decomposition, degrading, exoelectrogen Biology (General) QH301-705.5 Nur Syafira Khoirunnisa SYAIFUL ANWAR DWI ANDREAS SANTOSA Isolation and selection of cellulolytic bacteria from rice straw for consortium of microbial fuel cell
description	Abstract. Khoirunnisa NS, Anwar S, Santosa DA. 2020. Isolation and selection of cellulolytic bacteria from rice straw for consortium of microbial fuel cell. Biodiversitas 21: 1686-1696. Cellulose such as in rice straw can be utilized as an organic substrate in Microbial Fuel Cell (MFC) to generate electricity by microorganisms as a biocatalyst. This research aimed to get cellulose-degrading bacteria with high capability to degrade rice straw and able to be used as consortium with exoelectrogen bacteria in Microbial Fuel Cell. The stages of research included: (i) isolation of the bacteria using carboxymethylcellulose (CMC) agar medium, (ii) selection of the isolates for that purpose, (iii) enzyme assay and MFC performance test, and (iv) identification of selected isolate. There were 125 isolates that were obtained. Selection based on the ability to degrade cellulose as indicated by clear zone on CMC medium resulted in 23 isolates. Ten isolates belong to anaerobic facultative bacteria were selected. Three of them were synergistic with exoelectrogen bacteria. The three isolates were tested for exoglucanase (Avicel) and total enzyme activity (Filter Paper) with the highest results were 6.21 U/mL (isolate J404) and 5.88 U/mL (isolate J401), respectively. The optimum MFC performance was achieved by one isolate, J401, which produced highest voltage of 40.8 mV and a power density of 0.33 mW/m2. The best isolate, J401, was identified as Xanthomonas translucens based on 16S rRNA method.
format	article
author	Nur Syafira Khoirunnisa SYAIFUL ANWAR DWI ANDREAS SANTOSA
author_facet	Nur Syafira Khoirunnisa SYAIFUL ANWAR DWI ANDREAS SANTOSA
author_sort	Nur Syafira Khoirunnisa
title	Isolation and selection of cellulolytic bacteria from rice straw for consortium of microbial fuel cell
title_short	Isolation and selection of cellulolytic bacteria from rice straw for consortium of microbial fuel cell
title_full	Isolation and selection of cellulolytic bacteria from rice straw for consortium of microbial fuel cell
title_fullStr	Isolation and selection of cellulolytic bacteria from rice straw for consortium of microbial fuel cell
title_full_unstemmed	Isolation and selection of cellulolytic bacteria from rice straw for consortium of microbial fuel cell
title_sort	isolation and selection of cellulolytic bacteria from rice straw for consortium of microbial fuel cell
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/248c9ec7ecf54140b6590edf9af94692
work_keys_str_mv	AT nursyafirakhoirunnisa isolationandselectionofcellulolyticbacteriafromricestrawforconsortiumofmicrobialfuelcell AT syaifulanwar isolationandselectionofcellulolyticbacteriafromricestrawforconsortiumofmicrobialfuelcell AT dwiandreassantosa isolationandselectionofcellulolyticbacteriafromricestrawforconsortiumofmicrobialfuelcell
_version_	1718418569871491072

Isolation and selection of cellulolytic bacteria from rice straw for consortium of microbial fuel cell

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