MiR-21 expression in the tumor stroma of oral squamous cell carcinoma: an independent biomarker of disease free survival.

Oral squamous cell carcinoma (OSCC) patients have a high mortality rate; thus, new clinical biomarkers and therapeutic options are needed. MicroRNAs (miRNAs) are short noncoding RNAs that regulate posttranscriptional gene expression and are commonly deregulated in OSCC and other cancers. MicroRNA-21...

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Autores principales:	Nora Hedbäck, David H Jensen, Lena Specht, Anne-Marie K Fiehn, Marianne H Therkildsen, Lennart Friis-Hansen, Erik Dabelsteen, Christian von Buchwald
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/248e8c6304214b7a87488a6e6a7d510b
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spelling	oai:doaj.org-article:248e8c6304214b7a87488a6e6a7d510b2021-11-18T08:21:56ZMiR-21 expression in the tumor stroma of oral squamous cell carcinoma: an independent biomarker of disease free survival.1932-620310.1371/journal.pone.0095193https://doaj.org/article/248e8c6304214b7a87488a6e6a7d510b2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24755828/?tool=EBIhttps://doaj.org/toc/1932-6203Oral squamous cell carcinoma (OSCC) patients have a high mortality rate; thus, new clinical biomarkers and therapeutic options are needed. MicroRNAs (miRNAs) are short noncoding RNAs that regulate posttranscriptional gene expression and are commonly deregulated in OSCC and other cancers. MicroRNA-21 (miR-21) is the most consistently overexpressed miRNA in several types of cancer, and it might be a useful clinical biomarker and therapeutic target. To better understand the role of miR-21 in OSCC, paraffin-embedded tumor tissue samples from 86 patients with primary OSCC were analyzed by in situ hybridization. We found that miR-21 was primarily expressed in the tumor stroma and in some tumor-associated blood vessels with no expression in the adjacent normal epithelia or stroma. Using image analysis, we quantitatively estimated miR-21 expression levels specifically in the stroma of a cohort of OSCC samples. These miR-21 levels significantly correlated with disease free survival with the highest levels being located in the stroma. Stromal miR-21 expression was independently associated with a poorer prognosis, even after adjusting for clinical parameters (perineural invasion and N-stage) in a multivariate analysis. In summary, we have shown that miR-21 is located in the carcinoma cells, stroma and blood vessels of tumors, and its expression specifically in the stromal compartment has a negative prognostic value in OSCC.Nora HedbäckDavid H JensenLena SpechtAnne-Marie K FiehnMarianne H TherkildsenLennart Friis-HansenErik DabelsteenChristian von BuchwaldPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e95193 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nora Hedbäck David H Jensen Lena Specht Anne-Marie K Fiehn Marianne H Therkildsen Lennart Friis-Hansen Erik Dabelsteen Christian von Buchwald MiR-21 expression in the tumor stroma of oral squamous cell carcinoma: an independent biomarker of disease free survival.
description	Oral squamous cell carcinoma (OSCC) patients have a high mortality rate; thus, new clinical biomarkers and therapeutic options are needed. MicroRNAs (miRNAs) are short noncoding RNAs that regulate posttranscriptional gene expression and are commonly deregulated in OSCC and other cancers. MicroRNA-21 (miR-21) is the most consistently overexpressed miRNA in several types of cancer, and it might be a useful clinical biomarker and therapeutic target. To better understand the role of miR-21 in OSCC, paraffin-embedded tumor tissue samples from 86 patients with primary OSCC were analyzed by in situ hybridization. We found that miR-21 was primarily expressed in the tumor stroma and in some tumor-associated blood vessels with no expression in the adjacent normal epithelia or stroma. Using image analysis, we quantitatively estimated miR-21 expression levels specifically in the stroma of a cohort of OSCC samples. These miR-21 levels significantly correlated with disease free survival with the highest levels being located in the stroma. Stromal miR-21 expression was independently associated with a poorer prognosis, even after adjusting for clinical parameters (perineural invasion and N-stage) in a multivariate analysis. In summary, we have shown that miR-21 is located in the carcinoma cells, stroma and blood vessels of tumors, and its expression specifically in the stromal compartment has a negative prognostic value in OSCC.
format	article
author	Nora Hedbäck David H Jensen Lena Specht Anne-Marie K Fiehn Marianne H Therkildsen Lennart Friis-Hansen Erik Dabelsteen Christian von Buchwald
author_facet	Nora Hedbäck David H Jensen Lena Specht Anne-Marie K Fiehn Marianne H Therkildsen Lennart Friis-Hansen Erik Dabelsteen Christian von Buchwald
author_sort	Nora Hedbäck
title	MiR-21 expression in the tumor stroma of oral squamous cell carcinoma: an independent biomarker of disease free survival.
title_short	MiR-21 expression in the tumor stroma of oral squamous cell carcinoma: an independent biomarker of disease free survival.
title_full	MiR-21 expression in the tumor stroma of oral squamous cell carcinoma: an independent biomarker of disease free survival.
title_fullStr	MiR-21 expression in the tumor stroma of oral squamous cell carcinoma: an independent biomarker of disease free survival.
title_full_unstemmed	MiR-21 expression in the tumor stroma of oral squamous cell carcinoma: an independent biomarker of disease free survival.
title_sort	mir-21 expression in the tumor stroma of oral squamous cell carcinoma: an independent biomarker of disease free survival.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/248e8c6304214b7a87488a6e6a7d510b
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MiR-21 expression in the tumor stroma of oral squamous cell carcinoma: an independent biomarker of disease free survival.

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