High-throughput sequencing of microRNAs in peripheral blood mononuclear cells: identification of potential weight loss biomarkers.

<h4>Introduction</h4>MicroRNAs (miRNAs) are being increasingly studied in relation to energy metabolism and body composition homeostasis. Indeed, the quantitative analysis of miRNAs expression in different adiposity conditions may contribute to understand the intimate mechanisms particip...

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Autores principales:	Fermín I Milagro, Jonatan Miranda, María P Portillo, Alfredo Fernandez-Quintela, Javier Campión, J Alfredo Martínez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/249266a9f11e4ede9375d250dd6f9951
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spelling	oai:doaj.org-article:249266a9f11e4ede9375d250dd6f99512021-11-18T08:01:23ZHigh-throughput sequencing of microRNAs in peripheral blood mononuclear cells: identification of potential weight loss biomarkers.1932-620310.1371/journal.pone.0054319https://doaj.org/article/249266a9f11e4ede9375d250dd6f99512013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23335998/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Introduction</h4>MicroRNAs (miRNAs) are being increasingly studied in relation to energy metabolism and body composition homeostasis. Indeed, the quantitative analysis of miRNAs expression in different adiposity conditions may contribute to understand the intimate mechanisms participating in body weight control and to find new biomarkers with diagnostic or prognostic value in obesity management.<h4>Objective</h4>The aim of this study was the search for miRNAs in blood cells whose expression could be used as prognostic biomarkers of weight loss.<h4>Methods</h4>Ten Caucasian obese women were selected among the participants in a weight-loss trial that consisted in following an energy-restricted treatment. Weight loss was considered unsuccessful when <5% of initial body weight (non-responders) and successful when >5% (responders). At baseline, total miRNA isolated from peripheral blood mononuclear cells (PBMC) was sequenced with SOLiD v4. The miRNA sequencing data were validated by RT-PCR.<h4>Results</h4>Differential baseline expression of several miRNAs was found between responders and non-responders. Two miRNAs were up-regulated in the non-responder group (mir-935 and mir-4772) and three others were down-regulated (mir-223, mir-224 and mir-376b). Both mir-935 and mir-4772 showed relevant associations with the magnitude of weight loss, although the expression of other transcripts (mir-874, mir-199b, mir-766, mir-589 and mir-148b) also correlated with weight loss.<h4>Conclusions</h4>This research addresses the use of high-throughput sequencing technologies in the search for miRNA expression biomarkers in obesity, by determining the miRNA transcriptome of PBMC. Basal expression of different miRNAs, particularly mir-935 and mir-4772, could be prognostic biomarkers and may forecast the response to a hypocaloric diet.Fermín I MilagroJonatan MirandaMaría P PortilloAlfredo Fernandez-QuintelaJavier CampiónJ Alfredo MartínezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e54319 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fermín I Milagro Jonatan Miranda María P Portillo Alfredo Fernandez-Quintela Javier Campión J Alfredo Martínez High-throughput sequencing of microRNAs in peripheral blood mononuclear cells: identification of potential weight loss biomarkers.
description	<h4>Introduction</h4>MicroRNAs (miRNAs) are being increasingly studied in relation to energy metabolism and body composition homeostasis. Indeed, the quantitative analysis of miRNAs expression in different adiposity conditions may contribute to understand the intimate mechanisms participating in body weight control and to find new biomarkers with diagnostic or prognostic value in obesity management.<h4>Objective</h4>The aim of this study was the search for miRNAs in blood cells whose expression could be used as prognostic biomarkers of weight loss.<h4>Methods</h4>Ten Caucasian obese women were selected among the participants in a weight-loss trial that consisted in following an energy-restricted treatment. Weight loss was considered unsuccessful when <5% of initial body weight (non-responders) and successful when >5% (responders). At baseline, total miRNA isolated from peripheral blood mononuclear cells (PBMC) was sequenced with SOLiD v4. The miRNA sequencing data were validated by RT-PCR.<h4>Results</h4>Differential baseline expression of several miRNAs was found between responders and non-responders. Two miRNAs were up-regulated in the non-responder group (mir-935 and mir-4772) and three others were down-regulated (mir-223, mir-224 and mir-376b). Both mir-935 and mir-4772 showed relevant associations with the magnitude of weight loss, although the expression of other transcripts (mir-874, mir-199b, mir-766, mir-589 and mir-148b) also correlated with weight loss.<h4>Conclusions</h4>This research addresses the use of high-throughput sequencing technologies in the search for miRNA expression biomarkers in obesity, by determining the miRNA transcriptome of PBMC. Basal expression of different miRNAs, particularly mir-935 and mir-4772, could be prognostic biomarkers and may forecast the response to a hypocaloric diet.
format	article
author	Fermín I Milagro Jonatan Miranda María P Portillo Alfredo Fernandez-Quintela Javier Campión J Alfredo Martínez
author_facet	Fermín I Milagro Jonatan Miranda María P Portillo Alfredo Fernandez-Quintela Javier Campión J Alfredo Martínez
author_sort	Fermín I Milagro
title	High-throughput sequencing of microRNAs in peripheral blood mononuclear cells: identification of potential weight loss biomarkers.
title_short	High-throughput sequencing of microRNAs in peripheral blood mononuclear cells: identification of potential weight loss biomarkers.
title_full	High-throughput sequencing of microRNAs in peripheral blood mononuclear cells: identification of potential weight loss biomarkers.
title_fullStr	High-throughput sequencing of microRNAs in peripheral blood mononuclear cells: identification of potential weight loss biomarkers.
title_full_unstemmed	High-throughput sequencing of microRNAs in peripheral blood mononuclear cells: identification of potential weight loss biomarkers.
title_sort	high-throughput sequencing of micrornas in peripheral blood mononuclear cells: identification of potential weight loss biomarkers.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/249266a9f11e4ede9375d250dd6f9951
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High-throughput sequencing of microRNAs in peripheral blood mononuclear cells: identification of potential weight loss biomarkers.

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