Short Communication: Identification and characterization of nitrifying bacteria in mud crab (Scylla serrata) recirculation aquaculture system by 16S rRNA sequencing

Abstract. Hastuti YP, Rusmana I, Nirmala K, Affandi R, Tridesianti S. 2019. Identification and characterization of nitrifying bacteria in mud crab (Scylla serrata) recirculation aquaculture system by 16S rRNA sequencing. Biodiversitas 20: 1339-1343. Recirculation is one of the systems used for incre...

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Autores principales:	YUNI PUJI HASTUTI, IMAN RUSMANA, KUKUH NIRMALA, RIDWAN AFFANDI, SISKA TRIDESIANTI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	scylla serrata, identification, characterization, bacteria, nitrification Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/24a210ff4ee74dd7b4cb2ffbc258ebad
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spelling	oai:doaj.org-article:24a210ff4ee74dd7b4cb2ffbc258ebad2021-11-21T21:40:43ZShort Communication: Identification and characterization of nitrifying bacteria in mud crab (Scylla serrata) recirculation aquaculture system by 16S rRNA sequencing1412-033X2085-472210.13057/biodiv/d200524https://doaj.org/article/24a210ff4ee74dd7b4cb2ffbc258ebad2019-04-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2882https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Hastuti YP, Rusmana I, Nirmala K, Affandi R, Tridesianti S. 2019. Identification and characterization of nitrifying bacteria in mud crab (Scylla serrata) recirculation aquaculture system by 16S rRNA sequencing. Biodiversitas 20: 1339-1343. Recirculation is one of the systems used for increasing aquaculture production, for example, is mud crab Scylla serrata culture. Aquaculture activities will produce organic nitrogen waste and can be affected to inorganic condition (nitrification bacteria). Nitrifying bacteria are capable of changing aerobically from NH4+ to NO2-(nitrite) and from NO2-to NO3-(nitrate). This current work aimed to identify and characterize nitrifying bacteria in mud crab (S. serrata) culture with a recirculated system. Nitrifying bacteria were isolated using serial dilution (10-1 until 10-5) of open filter FB water sample previously cultured in a specific medium. Bacterial identification was performed using a procedure of PrestoTM Mini gDNA Kit (Geneid) for DNA extraction and Polymerase Chain Reaction (PCR) primer 63F and 1387R for 16S rRNA gene amplification. Molecular identification using 16S rRNA sequences, the nitrifying bacteria isolated from mud crab recirculation aquaculture system were 99% identified as a group Pantoea calida, Pseudomonas stutzerii dan Halomonas sp. Morphological characterization of the colony morphology showed that a mucoid appearance and appeared cream in color and large size. Furthermore, their cells were recognized as basil and Gram-negative.YUNI PUJI HASTUTIIMAN RUSMANAKUKUH NIRMALARIDWAN AFFANDISISKA TRIDESIANTIMBI & UNS Soloarticlescylla serrata, identification, characterization, bacteria, nitrificationBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 5 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	scylla serrata, identification, characterization, bacteria, nitrification Biology (General) QH301-705.5
spellingShingle	scylla serrata, identification, characterization, bacteria, nitrification Biology (General) QH301-705.5 YUNI PUJI HASTUTI IMAN RUSMANA KUKUH NIRMALA RIDWAN AFFANDI SISKA TRIDESIANTI Short Communication: Identification and characterization of nitrifying bacteria in mud crab (Scylla serrata) recirculation aquaculture system by 16S rRNA sequencing
description	Abstract. Hastuti YP, Rusmana I, Nirmala K, Affandi R, Tridesianti S. 2019. Identification and characterization of nitrifying bacteria in mud crab (Scylla serrata) recirculation aquaculture system by 16S rRNA sequencing. Biodiversitas 20: 1339-1343. Recirculation is one of the systems used for increasing aquaculture production, for example, is mud crab Scylla serrata culture. Aquaculture activities will produce organic nitrogen waste and can be affected to inorganic condition (nitrification bacteria). Nitrifying bacteria are capable of changing aerobically from NH4+ to NO2-(nitrite) and from NO2-to NO3-(nitrate). This current work aimed to identify and characterize nitrifying bacteria in mud crab (S. serrata) culture with a recirculated system. Nitrifying bacteria were isolated using serial dilution (10-1 until 10-5) of open filter FB water sample previously cultured in a specific medium. Bacterial identification was performed using a procedure of PrestoTM Mini gDNA Kit (Geneid) for DNA extraction and Polymerase Chain Reaction (PCR) primer 63F and 1387R for 16S rRNA gene amplification. Molecular identification using 16S rRNA sequences, the nitrifying bacteria isolated from mud crab recirculation aquaculture system were 99% identified as a group Pantoea calida, Pseudomonas stutzerii dan Halomonas sp. Morphological characterization of the colony morphology showed that a mucoid appearance and appeared cream in color and large size. Furthermore, their cells were recognized as basil and Gram-negative.
format	article
author	YUNI PUJI HASTUTI IMAN RUSMANA KUKUH NIRMALA RIDWAN AFFANDI SISKA TRIDESIANTI
author_facet	YUNI PUJI HASTUTI IMAN RUSMANA KUKUH NIRMALA RIDWAN AFFANDI SISKA TRIDESIANTI
author_sort	YUNI PUJI HASTUTI
title	Short Communication: Identification and characterization of nitrifying bacteria in mud crab (Scylla serrata) recirculation aquaculture system by 16S rRNA sequencing
title_short	Short Communication: Identification and characterization of nitrifying bacteria in mud crab (Scylla serrata) recirculation aquaculture system by 16S rRNA sequencing
title_full	Short Communication: Identification and characterization of nitrifying bacteria in mud crab (Scylla serrata) recirculation aquaculture system by 16S rRNA sequencing
title_fullStr	Short Communication: Identification and characterization of nitrifying bacteria in mud crab (Scylla serrata) recirculation aquaculture system by 16S rRNA sequencing
title_full_unstemmed	Short Communication: Identification and characterization of nitrifying bacteria in mud crab (Scylla serrata) recirculation aquaculture system by 16S rRNA sequencing
title_sort	short communication: identification and characterization of nitrifying bacteria in mud crab (scylla serrata) recirculation aquaculture system by 16s rrna sequencing
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/24a210ff4ee74dd7b4cb2ffbc258ebad
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Short Communication: Identification and characterization of nitrifying bacteria in mud crab (Scylla serrata) recirculation aquaculture system by 16S rRNA sequencing

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