Proteomic profiling of the rat hippocampus from the kindling and pilocarpine models of epilepsy: potential targets in calcium regulatory network

Abstract Herein proteomic profiling of the rat hippocampus from the kindling and pilocarpine models of epilepsy was performed to achieve new potential targets for treating epileptic seizures. A total of 144 differently expressed proteins in both left and right hippocampi by two-dimensional electroph...

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Autores principales:	Leila Sadeghi, Albert Anatolyevich Rizvanov, Bahareh Dabirmanesh, Ilnur Ildusovich Salafutdinov, Mohammad Sayyah, Amir Shojaei, Javad Zahiri, Javad Mirnajafi-Zadeh, Babak Khorsand, Khosro Khajeh, Yaghoub Fathollahi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/24a6ff8b58234294b17887def9b2d64f
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spelling	oai:doaj.org-article:24a6ff8b58234294b17887def9b2d64f2021-12-02T15:51:12ZProteomic profiling of the rat hippocampus from the kindling and pilocarpine models of epilepsy: potential targets in calcium regulatory network10.1038/s41598-021-87555-72045-2322https://doaj.org/article/24a6ff8b58234294b17887def9b2d64f2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87555-7https://doaj.org/toc/2045-2322Abstract Herein proteomic profiling of the rat hippocampus from the kindling and pilocarpine models of epilepsy was performed to achieve new potential targets for treating epileptic seizures. A total of 144 differently expressed proteins in both left and right hippocampi by two-dimensional electrophoresis coupled to matrix-assisted laser desorption-mass spectrometry were identified across the rat models of epilepsy. Based on network analysis, the majority of differentially expressed proteins were associated with Ca2+ homeostasis. Changes in ADP-ribosyl cyclase (ADPRC), lysophosphatidic acid receptor 3 (LPAR3), calreticulin, ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1), synaptosomal nerve-associated protein 25 (SNAP 25) and transgelin 3 proteins were probed by Western blot analysis and validated using immunohistochemistry. Inhibition of calcium influx by 8-Bromo-cADP-Ribose (8-Br-cADPR) and 2-Aminoethyl diphenylborinate (2-APB) which act via the ADPRC and LPAR3, respectively, attenuated epileptic seizures. Considering a wide range of molecular events and effective role of calcium homeostasis in epilepsy, polypharmacy with multiple realistic targets should be further explored to reach the most effective treatments.Leila SadeghiAlbert Anatolyevich RizvanovBahareh DabirmaneshIlnur Ildusovich SalafutdinovMohammad SayyahAmir ShojaeiJavad ZahiriJavad Mirnajafi-ZadehBabak KhorsandKhosro KhajehYaghoub FathollahiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Leila Sadeghi Albert Anatolyevich Rizvanov Bahareh Dabirmanesh Ilnur Ildusovich Salafutdinov Mohammad Sayyah Amir Shojaei Javad Zahiri Javad Mirnajafi-Zadeh Babak Khorsand Khosro Khajeh Yaghoub Fathollahi Proteomic profiling of the rat hippocampus from the kindling and pilocarpine models of epilepsy: potential targets in calcium regulatory network
description	Abstract Herein proteomic profiling of the rat hippocampus from the kindling and pilocarpine models of epilepsy was performed to achieve new potential targets for treating epileptic seizures. A total of 144 differently expressed proteins in both left and right hippocampi by two-dimensional electrophoresis coupled to matrix-assisted laser desorption-mass spectrometry were identified across the rat models of epilepsy. Based on network analysis, the majority of differentially expressed proteins were associated with Ca2+ homeostasis. Changes in ADP-ribosyl cyclase (ADPRC), lysophosphatidic acid receptor 3 (LPAR3), calreticulin, ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1), synaptosomal nerve-associated protein 25 (SNAP 25) and transgelin 3 proteins were probed by Western blot analysis and validated using immunohistochemistry. Inhibition of calcium influx by 8-Bromo-cADP-Ribose (8-Br-cADPR) and 2-Aminoethyl diphenylborinate (2-APB) which act via the ADPRC and LPAR3, respectively, attenuated epileptic seizures. Considering a wide range of molecular events and effective role of calcium homeostasis in epilepsy, polypharmacy with multiple realistic targets should be further explored to reach the most effective treatments.
format	article
author	Leila Sadeghi Albert Anatolyevich Rizvanov Bahareh Dabirmanesh Ilnur Ildusovich Salafutdinov Mohammad Sayyah Amir Shojaei Javad Zahiri Javad Mirnajafi-Zadeh Babak Khorsand Khosro Khajeh Yaghoub Fathollahi
author_facet	Leila Sadeghi Albert Anatolyevich Rizvanov Bahareh Dabirmanesh Ilnur Ildusovich Salafutdinov Mohammad Sayyah Amir Shojaei Javad Zahiri Javad Mirnajafi-Zadeh Babak Khorsand Khosro Khajeh Yaghoub Fathollahi
author_sort	Leila Sadeghi
title	Proteomic profiling of the rat hippocampus from the kindling and pilocarpine models of epilepsy: potential targets in calcium regulatory network
title_short	Proteomic profiling of the rat hippocampus from the kindling and pilocarpine models of epilepsy: potential targets in calcium regulatory network
title_full	Proteomic profiling of the rat hippocampus from the kindling and pilocarpine models of epilepsy: potential targets in calcium regulatory network
title_fullStr	Proteomic profiling of the rat hippocampus from the kindling and pilocarpine models of epilepsy: potential targets in calcium regulatory network
title_full_unstemmed	Proteomic profiling of the rat hippocampus from the kindling and pilocarpine models of epilepsy: potential targets in calcium regulatory network
title_sort	proteomic profiling of the rat hippocampus from the kindling and pilocarpine models of epilepsy: potential targets in calcium regulatory network
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/24a6ff8b58234294b17887def9b2d64f
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Proteomic profiling of the rat hippocampus from the kindling and pilocarpine models of epilepsy: potential targets in calcium regulatory network

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