Effects of In Vitro Muscle Contraction on Thermogenic Protein Levels in Co-Cultured Adipocytes

The crosstalk between the exercising muscle and the adipose tissue, mediated by myokines and metabolites, derived from both tissues during exercise has created a controversy between animal and human studies with respect to the impact of exercise on the browning process. The aim of this study was to...

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Autores principales:	Eleni Nintou, Eleni Karligiotou, Maria Vliora, Ioannis G. Fatouros, Athanasios Z. Jamurtas, Nikos Sakellaridis, Konstantinos Dimas, Andreas D. Flouris
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	adipocytes EPS myotubes thermogenesis UCP1 muscle contraction Science Q
Acceso en línea:	https://doaj.org/article/24ad2f9ac9d7455ca4e9c071e596dba9
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id	oai:doaj.org-article:24ad2f9ac9d7455ca4e9c071e596dba9
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spelling	oai:doaj.org-article:24ad2f9ac9d7455ca4e9c071e596dba92021-11-25T18:11:21ZEffects of In Vitro Muscle Contraction on Thermogenic Protein Levels in Co-Cultured Adipocytes10.3390/life111112272075-1729https://doaj.org/article/24ad2f9ac9d7455ca4e9c071e596dba92021-11-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1227https://doaj.org/toc/2075-1729The crosstalk between the exercising muscle and the adipose tissue, mediated by myokines and metabolites, derived from both tissues during exercise has created a controversy between animal and human studies with respect to the impact of exercise on the browning process. The aim of this study was to investigate whether co-culturing of C2C12 myotubes and 3T3-L1 adipocytes under the stimuli of electrical pulse stimulation (EPS) mimicking muscle contraction can impact the expression of UCP1, PGC-1a, and IL-6 in adipocytes, therefore providing evidence on the direct crosstalk between adipocytes and stimulated muscle cells. In the co-cultured C2C12 cells, EPS increased the expression of PGC-1a (<i>p</i> = 0.129; d = 0.73) and IL-6 (<i>p</i> = 0.09; d = 1.13) protein levels. When EPS was applied, we found that co-culturing led to increases in UCP1 (<i>p</i> = 0.044; d = 1.29) and IL-6 (<i>p</i> = 0.097; d = 1.13) protein expression in the 3T3-L1 adipocytes. The expression of PGC-1a increased by EPS but was not significantly elevated after co-culturing (<i>p</i> = 0.448; d = 0.08). In vitro co-culturing of C2C12 myotubes and 3T3-L1 adipocytes under the stimuli of EPS leads to increased expression of thermogenic proteins. These findings indicate changes in the expression pattern of proteins related to browning of adipose tissue, supporting the use of this in vitro model to study the crosstalk between adipocytes and contracting muscle.Eleni NintouEleni KarligiotouMaria VlioraIoannis G. FatourosAthanasios Z. JamurtasNikos SakellaridisKonstantinos DimasAndreas D. FlourisMDPI AGarticleadipocytesEPSmyotubesthermogenesisUCP1muscle contractionScienceQENLife, Vol 11, Iss 1227, p 1227 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adipocytes EPS myotubes thermogenesis UCP1 muscle contraction Science Q
spellingShingle	adipocytes EPS myotubes thermogenesis UCP1 muscle contraction Science Q Eleni Nintou Eleni Karligiotou Maria Vliora Ioannis G. Fatouros Athanasios Z. Jamurtas Nikos Sakellaridis Konstantinos Dimas Andreas D. Flouris Effects of In Vitro Muscle Contraction on Thermogenic Protein Levels in Co-Cultured Adipocytes
description	The crosstalk between the exercising muscle and the adipose tissue, mediated by myokines and metabolites, derived from both tissues during exercise has created a controversy between animal and human studies with respect to the impact of exercise on the browning process. The aim of this study was to investigate whether co-culturing of C2C12 myotubes and 3T3-L1 adipocytes under the stimuli of electrical pulse stimulation (EPS) mimicking muscle contraction can impact the expression of UCP1, PGC-1a, and IL-6 in adipocytes, therefore providing evidence on the direct crosstalk between adipocytes and stimulated muscle cells. In the co-cultured C2C12 cells, EPS increased the expression of PGC-1a (<i>p</i> = 0.129; d = 0.73) and IL-6 (<i>p</i> = 0.09; d = 1.13) protein levels. When EPS was applied, we found that co-culturing led to increases in UCP1 (<i>p</i> = 0.044; d = 1.29) and IL-6 (<i>p</i> = 0.097; d = 1.13) protein expression in the 3T3-L1 adipocytes. The expression of PGC-1a increased by EPS but was not significantly elevated after co-culturing (<i>p</i> = 0.448; d = 0.08). In vitro co-culturing of C2C12 myotubes and 3T3-L1 adipocytes under the stimuli of EPS leads to increased expression of thermogenic proteins. These findings indicate changes in the expression pattern of proteins related to browning of adipose tissue, supporting the use of this in vitro model to study the crosstalk between adipocytes and contracting muscle.
format	article
author	Eleni Nintou Eleni Karligiotou Maria Vliora Ioannis G. Fatouros Athanasios Z. Jamurtas Nikos Sakellaridis Konstantinos Dimas Andreas D. Flouris
author_facet	Eleni Nintou Eleni Karligiotou Maria Vliora Ioannis G. Fatouros Athanasios Z. Jamurtas Nikos Sakellaridis Konstantinos Dimas Andreas D. Flouris
author_sort	Eleni Nintou
title	Effects of In Vitro Muscle Contraction on Thermogenic Protein Levels in Co-Cultured Adipocytes
title_short	Effects of In Vitro Muscle Contraction on Thermogenic Protein Levels in Co-Cultured Adipocytes
title_full	Effects of In Vitro Muscle Contraction on Thermogenic Protein Levels in Co-Cultured Adipocytes
title_fullStr	Effects of In Vitro Muscle Contraction on Thermogenic Protein Levels in Co-Cultured Adipocytes
title_full_unstemmed	Effects of In Vitro Muscle Contraction on Thermogenic Protein Levels in Co-Cultured Adipocytes
title_sort	effects of in vitro muscle contraction on thermogenic protein levels in co-cultured adipocytes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/24ad2f9ac9d7455ca4e9c071e596dba9
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_version_	1718411486232051712

Effects of In Vitro Muscle Contraction on Thermogenic Protein Levels in Co-Cultured Adipocytes

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