Multiple cargo deliveries of growth factors and antimicrobial peptide using biodegradable nanopolymer as a potential wound healing system

Amritha Vijayan, Pinky Prabha James, CK Nanditha, GS Vinod Kumar Chemical Biology, Nano Drug Delivery Systems (NDDS), Bio-Innovation Center (BIC), Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala 695014, India Background: Treatment of wounds with the help of nanoparticles (NPs) is...

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Autores principales:	Vijayan A, James PP, Nanditha CK, Kumar GSV
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	growth factors VEGF bFGF PLGA antimicrobial peptides Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/24bd80f73b9b4a1c8474df6e3d4262a8
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spelling	oai:doaj.org-article:24bd80f73b9b4a1c8474df6e3d4262a82021-12-02T03:23:23ZMultiple cargo deliveries of growth factors and antimicrobial peptide using biodegradable nanopolymer as a potential wound healing system1178-2013https://doaj.org/article/24bd80f73b9b4a1c8474df6e3d4262a82019-03-01T00:00:00Zhttps://www.dovepress.com/multiple-cargo-deliveries-of-growth-factors-and-antimicrobial-peptide--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Amritha Vijayan, Pinky Prabha James, CK Nanditha, GS Vinod Kumar Chemical Biology, Nano Drug Delivery Systems (NDDS), Bio-Innovation Center (BIC), Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala 695014, India Background: Treatment of wounds with the help of nanoparticles (NPs) is more effective and superior in comparison to traditional wound healing methods as it protects and sustains active drug release at the wound site thus enhancing the safety of the drug and reducing the possibility of side effects. The advantages of this method are the possibility of allowing a reduction in administered dose, limiting toxicity levels to the minimum, and increasing safety of topical delivery of the drug.Materials and methods: We report the synthesis of a novel poly (lactic-co-glycolic acid) (PLGA) NP-based multicargo delivery system for growth factors and antimicrobial peptide. Growth factors vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) were entrapped in PLGA NPs by solvent diffusion method and an antimicrobial peptide (K4) was conjugated to the NP by carbodiimide chemistry. The developed multiple cargo delivery systems with growth factors (VEGF and bFGF) and an antimicrobial peptide (K4) were investigated and optimized for potential wound healing.Results: The system showed a sustained release of growth factors and was evaluated for cytotoxicity by MTT and live/dead assay, which revealed that the bioactivity of the growth factor-entrapped NPs was higher than that of free growth factors, and it also induced enhanced cell proliferation in vitro.Conclusion: The development of a system for the codelivery of growth factors (VEGF and bFGF) and an antimicrobial peptide (K4) was investigated for potential wound healing application. The entrapment of growth factors with very high efficiency is an advantage in this method along with its sustained release from the nanoparticulate system, which will enhance the angiogenesis. Our system also displayed broad-spectrum antimicrobial activity against both gram-positive and gram-negative bacteria. Keywords: growth factors, VEGF, bFGF, PLGA, antimicrobial peptidesVijayan AJames PPNanditha CKKumar GSVDove Medical Pressarticlegrowth factorsVEGFbFGFPLGAantimicrobial peptidesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 2253-2263 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	growth factors VEGF bFGF PLGA antimicrobial peptides Medicine (General) R5-920
spellingShingle	growth factors VEGF bFGF PLGA antimicrobial peptides Medicine (General) R5-920 Vijayan A James PP Nanditha CK Kumar GSV Multiple cargo deliveries of growth factors and antimicrobial peptide using biodegradable nanopolymer as a potential wound healing system
description	Amritha Vijayan, Pinky Prabha James, CK Nanditha, GS Vinod Kumar Chemical Biology, Nano Drug Delivery Systems (NDDS), Bio-Innovation Center (BIC), Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala 695014, India Background: Treatment of wounds with the help of nanoparticles (NPs) is more effective and superior in comparison to traditional wound healing methods as it protects and sustains active drug release at the wound site thus enhancing the safety of the drug and reducing the possibility of side effects. The advantages of this method are the possibility of allowing a reduction in administered dose, limiting toxicity levels to the minimum, and increasing safety of topical delivery of the drug.Materials and methods: We report the synthesis of a novel poly (lactic-co-glycolic acid) (PLGA) NP-based multicargo delivery system for growth factors and antimicrobial peptide. Growth factors vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) were entrapped in PLGA NPs by solvent diffusion method and an antimicrobial peptide (K4) was conjugated to the NP by carbodiimide chemistry. The developed multiple cargo delivery systems with growth factors (VEGF and bFGF) and an antimicrobial peptide (K4) were investigated and optimized for potential wound healing.Results: The system showed a sustained release of growth factors and was evaluated for cytotoxicity by MTT and live/dead assay, which revealed that the bioactivity of the growth factor-entrapped NPs was higher than that of free growth factors, and it also induced enhanced cell proliferation in vitro.Conclusion: The development of a system for the codelivery of growth factors (VEGF and bFGF) and an antimicrobial peptide (K4) was investigated for potential wound healing application. The entrapment of growth factors with very high efficiency is an advantage in this method along with its sustained release from the nanoparticulate system, which will enhance the angiogenesis. Our system also displayed broad-spectrum antimicrobial activity against both gram-positive and gram-negative bacteria. Keywords: growth factors, VEGF, bFGF, PLGA, antimicrobial peptides
format	article
author	Vijayan A James PP Nanditha CK Kumar GSV
author_facet	Vijayan A James PP Nanditha CK Kumar GSV
author_sort	Vijayan A
title	Multiple cargo deliveries of growth factors and antimicrobial peptide using biodegradable nanopolymer as a potential wound healing system
title_short	Multiple cargo deliveries of growth factors and antimicrobial peptide using biodegradable nanopolymer as a potential wound healing system
title_full	Multiple cargo deliveries of growth factors and antimicrobial peptide using biodegradable nanopolymer as a potential wound healing system
title_fullStr	Multiple cargo deliveries of growth factors and antimicrobial peptide using biodegradable nanopolymer as a potential wound healing system
title_full_unstemmed	Multiple cargo deliveries of growth factors and antimicrobial peptide using biodegradable nanopolymer as a potential wound healing system
title_sort	multiple cargo deliveries of growth factors and antimicrobial peptide using biodegradable nanopolymer as a potential wound healing system
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/24bd80f73b9b4a1c8474df6e3d4262a8
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Multiple cargo deliveries of growth factors and antimicrobial peptide using biodegradable nanopolymer as a potential wound healing system

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