Immunoreactivity of Polish Lyme Disease Patient Sera to Specific <i>Borrelia</i> Antigens—Part 1

The diverse clinical picture and the non-specificity of symptoms in Lyme disease (LD) require the implementation of effective diagnostics, which should take into account the heterogeneity of <i>Borrelia</i> antigens. According to available guidelines, laboratories should use a two-tier s...

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Autores principales:	Iwona Wojciechowska-Koszko, Magdalena Mnichowska-Polanowska, Paweł Kwiatkowski, Paulina Roszkowska, Monika Sienkiewicz, Barbara Dołęgowska
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Lyme disease immunoreactivity ELISA immunoblot <i>Borrelia</i> Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/24c063deb7fe41218dffa1fff1f9c9ab
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spelling	oai:doaj.org-article:24c063deb7fe41218dffa1fff1f9c9ab2021-11-25T17:22:08ZImmunoreactivity of Polish Lyme Disease Patient Sera to Specific <i>Borrelia</i> Antigens—Part 110.3390/diagnostics111121572075-4418https://doaj.org/article/24c063deb7fe41218dffa1fff1f9c9ab2021-11-01T00:00:00Zhttps://www.mdpi.com/2075-4418/11/11/2157https://doaj.org/toc/2075-4418The diverse clinical picture and the non-specificity of symptoms in Lyme disease (LD) require the implementation of effective diagnostics, which should take into account the heterogeneity of <i>Borrelia</i> antigens. According to available guidelines, laboratories should use a two-tier serological diagnosis based on the enzyme-linked immunosorbent (ELISA) screening test and confirmation of the immunoblot (IB). The aim of the study was to investigate the immunoreactivity of LD patient sera to <i>Borrelia</i> antigens and to attempt to identify the genospecies responsible for LD using an ELISA–IB assay combination. Eighty patients with suspected LD and 22 healthy people participated in the study. All samples were tested with ELISA and IB assays in both IgM and IgG antibodies. In the case of the ELISA assay, more positive results were obtained in the IgM class than in the IgG class. In the case of the IB assay, positive results dominated in the IgG class. Positive results obtained in the IB assay most often showed IgM antibodies against the OspC and flagellin antigens, whereas the IgG antibodies were against VlsE, BmpA, OspC, p41, and p83 antigens. The IB assay is an important part of LD serodiagnosis and should be mandatory in diagnostic laboratories.Iwona Wojciechowska-KoszkoMagdalena Mnichowska-PolanowskaPaweł KwiatkowskiPaulina RoszkowskaMonika SienkiewiczBarbara DołęgowskaMDPI AGarticleLyme diseaseimmunoreactivityELISAimmunoblot<i>Borrelia</i>Medicine (General)R5-920ENDiagnostics, Vol 11, Iss 2157, p 2157 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lyme disease immunoreactivity ELISA immunoblot <i>Borrelia</i> Medicine (General) R5-920
spellingShingle	Lyme disease immunoreactivity ELISA immunoblot <i>Borrelia</i> Medicine (General) R5-920 Iwona Wojciechowska-Koszko Magdalena Mnichowska-Polanowska Paweł Kwiatkowski Paulina Roszkowska Monika Sienkiewicz Barbara Dołęgowska Immunoreactivity of Polish Lyme Disease Patient Sera to Specific <i>Borrelia</i> Antigens—Part 1
description	The diverse clinical picture and the non-specificity of symptoms in Lyme disease (LD) require the implementation of effective diagnostics, which should take into account the heterogeneity of <i>Borrelia</i> antigens. According to available guidelines, laboratories should use a two-tier serological diagnosis based on the enzyme-linked immunosorbent (ELISA) screening test and confirmation of the immunoblot (IB). The aim of the study was to investigate the immunoreactivity of LD patient sera to <i>Borrelia</i> antigens and to attempt to identify the genospecies responsible for LD using an ELISA–IB assay combination. Eighty patients with suspected LD and 22 healthy people participated in the study. All samples were tested with ELISA and IB assays in both IgM and IgG antibodies. In the case of the ELISA assay, more positive results were obtained in the IgM class than in the IgG class. In the case of the IB assay, positive results dominated in the IgG class. Positive results obtained in the IB assay most often showed IgM antibodies against the OspC and flagellin antigens, whereas the IgG antibodies were against VlsE, BmpA, OspC, p41, and p83 antigens. The IB assay is an important part of LD serodiagnosis and should be mandatory in diagnostic laboratories.
format	article
author	Iwona Wojciechowska-Koszko Magdalena Mnichowska-Polanowska Paweł Kwiatkowski Paulina Roszkowska Monika Sienkiewicz Barbara Dołęgowska
author_facet	Iwona Wojciechowska-Koszko Magdalena Mnichowska-Polanowska Paweł Kwiatkowski Paulina Roszkowska Monika Sienkiewicz Barbara Dołęgowska
author_sort	Iwona Wojciechowska-Koszko
title	Immunoreactivity of Polish Lyme Disease Patient Sera to Specific <i>Borrelia</i> Antigens—Part 1
title_short	Immunoreactivity of Polish Lyme Disease Patient Sera to Specific <i>Borrelia</i> Antigens—Part 1
title_full	Immunoreactivity of Polish Lyme Disease Patient Sera to Specific <i>Borrelia</i> Antigens—Part 1
title_fullStr	Immunoreactivity of Polish Lyme Disease Patient Sera to Specific <i>Borrelia</i> Antigens—Part 1
title_full_unstemmed	Immunoreactivity of Polish Lyme Disease Patient Sera to Specific <i>Borrelia</i> Antigens—Part 1
title_sort	immunoreactivity of polish lyme disease patient sera to specific <i>borrelia</i> antigens—part 1
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/24c063deb7fe41218dffa1fff1f9c9ab
work_keys_str_mv	AT iwonawojciechowskakoszko immunoreactivityofpolishlymediseasepatientseratospecificiborreliaiantigenspart1 AT magdalenamnichowskapolanowska immunoreactivityofpolishlymediseasepatientseratospecificiborreliaiantigenspart1 AT pawełkwiatkowski immunoreactivityofpolishlymediseasepatientseratospecificiborreliaiantigenspart1 AT paulinaroszkowska immunoreactivityofpolishlymediseasepatientseratospecificiborreliaiantigenspart1 AT monikasienkiewicz immunoreactivityofpolishlymediseasepatientseratospecificiborreliaiantigenspart1 AT barbaradołegowska immunoreactivityofpolishlymediseasepatientseratospecificiborreliaiantigenspart1
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Immunoreactivity of Polish Lyme Disease Patient Sera to Specific <i>Borrelia</i> Antigens—Part 1

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