Shared and Unique Evolutionary Trajectories to Ciprofloxacin Resistance in Gram-Negative Bacterial Pathogens

The challenge of spreading antibiotic resistance calls for systematic efforts to develop more “irresistible” drugs based on a deeper understanding of dynamics and mechanisms of antibiotic resistance acquisition. To address this challenge, we have established a comparative resistomics approach which...

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Autores principales:	Jaime E. Zlamal, Semen A. Leyn, Mallika Iyer, Marinela L. Elane, Nicholas A. Wong, James W. Wamsley, Maarten Vercruysse, Fernando Garcia-Alcalde, Andrei L. Osterman
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/24c2bf31f14d4d6f8689b162ab43cd66
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spelling	oai:doaj.org-article:24c2bf31f14d4d6f8689b162ab43cd662021-11-03T21:15:04ZShared and Unique Evolutionary Trajectories to Ciprofloxacin Resistance in Gram-Negative Bacterial Pathogens2150-751110.1128/mBio.00987-21https://doaj.org/article/24c2bf31f14d4d6f8689b162ab43cd662021-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00987-21https://doaj.org/toc/2150-7511 The challenge of spreading antibiotic resistance calls for systematic efforts to develop more “irresistible” drugs based on a deeper understanding of dynamics and mechanisms of antibiotic resistance acquisition. To address this challenge, we have established a comparative resistomics approach which combines experimental evolution in a continuous-culturing device, the morbidostat, with ultradeep sequencing of evolving microbial populations to identify evolutionary trajectories (mutations and genome rearrangements) leading to antibiotic resistance over a range of target pathogens.Jaime E. ZlamalSemen A. LeynMallika IyerMarinela L. ElaneNicholas A. WongJames W. WamsleyMaarten VercruysseFernando Garcia-AlcaldeAndrei L. OstermanAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 12, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Jaime E. Zlamal Semen A. Leyn Mallika Iyer Marinela L. Elane Nicholas A. Wong James W. Wamsley Maarten Vercruysse Fernando Garcia-Alcalde Andrei L. Osterman Shared and Unique Evolutionary Trajectories to Ciprofloxacin Resistance in Gram-Negative Bacterial Pathogens
description	The challenge of spreading antibiotic resistance calls for systematic efforts to develop more “irresistible” drugs based on a deeper understanding of dynamics and mechanisms of antibiotic resistance acquisition. To address this challenge, we have established a comparative resistomics approach which combines experimental evolution in a continuous-culturing device, the morbidostat, with ultradeep sequencing of evolving microbial populations to identify evolutionary trajectories (mutations and genome rearrangements) leading to antibiotic resistance over a range of target pathogens.
format	article
author	Jaime E. Zlamal Semen A. Leyn Mallika Iyer Marinela L. Elane Nicholas A. Wong James W. Wamsley Maarten Vercruysse Fernando Garcia-Alcalde Andrei L. Osterman
author_facet	Jaime E. Zlamal Semen A. Leyn Mallika Iyer Marinela L. Elane Nicholas A. Wong James W. Wamsley Maarten Vercruysse Fernando Garcia-Alcalde Andrei L. Osterman
author_sort	Jaime E. Zlamal
title	Shared and Unique Evolutionary Trajectories to Ciprofloxacin Resistance in Gram-Negative Bacterial Pathogens
title_short	Shared and Unique Evolutionary Trajectories to Ciprofloxacin Resistance in Gram-Negative Bacterial Pathogens
title_full	Shared and Unique Evolutionary Trajectories to Ciprofloxacin Resistance in Gram-Negative Bacterial Pathogens
title_fullStr	Shared and Unique Evolutionary Trajectories to Ciprofloxacin Resistance in Gram-Negative Bacterial Pathogens
title_full_unstemmed	Shared and Unique Evolutionary Trajectories to Ciprofloxacin Resistance in Gram-Negative Bacterial Pathogens
title_sort	shared and unique evolutionary trajectories to ciprofloxacin resistance in gram-negative bacterial pathogens
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/24c2bf31f14d4d6f8689b162ab43cd66
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Shared and Unique Evolutionary Trajectories to Ciprofloxacin Resistance in Gram-Negative Bacterial Pathogens

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