Functional and Structural Characterization of <i>Pediococcus pentosaceus</i>-Derived Biosurfactant and Its Biomedical Potential against Bacterial Adhesion, Quorum Sensing, and Biofilm Formation

Biosurfactants are surface-active molecules of microbial origin and alternatives to synthetic surfactants with various applications. Due to their environmental-friendliness, biocompatibility, biodegradability, effectiveness to work under various environmental conditions, and non-toxic nature, they h...

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Autores principales:	Mohd Adnan, Arif Jamal Siddiqui, Walid Sabri Hamadou, Syed Amir Ashraf, Md Imtaiyaz Hassan, Mejdi Snoussi, Riadh Badraoui, Arshad Jamal, Fevzi Bardakci, Amir Mahgoub Awadelkareem, Manojkumar Sachidanandan, Mitesh Patel
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	biosurfactants <i>Pediococcus pentosaceus</i> antibiofilm anti-adhesion antimicrobial probiotic Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/24c3f463d4854f4fb48291aa59242da2
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spelling	oai:doaj.org-article:24c3f463d4854f4fb48291aa59242da22021-11-25T16:24:06ZFunctional and Structural Characterization of <i>Pediococcus pentosaceus</i>-Derived Biosurfactant and Its Biomedical Potential against Bacterial Adhesion, Quorum Sensing, and Biofilm Formation10.3390/antibiotics101113712079-6382https://doaj.org/article/24c3f463d4854f4fb48291aa59242da22021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1371https://doaj.org/toc/2079-6382Biosurfactants are surface-active molecules of microbial origin and alternatives to synthetic surfactants with various applications. Due to their environmental-friendliness, biocompatibility, biodegradability, effectiveness to work under various environmental conditions, and non-toxic nature, they have been recently recognized as potential agents with therapeutic and commercial importance. The biosurfactant produced by various probiotic lactic acid bacteria (LAB) has enormous applications in different fields. Thus, in vitro assessment of biofilm development prevention or disruption by natural biosurfactants derived from probiotic LAB is a plausible approach that can lead to the discovery of novel antimicrobials. Primarily, this study aims to isolate, screen, and characterize the functional and biomedical potential of biosurfactant synthesized by probiotic LAB <i>Pediococcus pentosaceus</i> (<i>P. pentosaceus</i>). Characterization consists of the assessment of critical micelle concentration (CMC), reduction in surface tension, and emulsification index (% EI24). Evaluation of antibacterial, antibiofilm, anti-QS, and anti-adhesive activities of cell-bound biosurfactants were carried out against different human pathogenic bacteria (<i>B. subtilis</i>, <i>P. aeruginosa</i>, <i>S. aureus</i>, and <i>E. coli</i>). Moreover, bacterial cell damage, viability of cells within the biofilm, and exopolysaccharide (EPS) production were also evaluated. As a result, <i>P. pentosaceus</i> was found to produce 4.75 ± 0.17 g/L biosurfactant, which displayed a CMC of 2.4 ± 0.68 g/L and reduced the surface tension from 71.11 ± 1.12 mN/m to 38.18 ± 0.58 mN/m. <i>P. pentosaceus</i> cells bound to the crude biosurfactant were found to be effective against all tested bacterial pathogens. It exhibited an anti-adhesion ability and impeded the architecture of the biofilm matrix by affecting the viability and integrity of bacterial cells within biofilms and reducing the total EPS content. Furthermore, the crude biosurfactant derived from <i>P. pentosaceus</i> was structurally characterized as a lipoprotein by GC-MS analysis, which confirms the presence of lipids and proteins. Thus, our findings represent the potent anti-adhesion and antibiofilm potential of <i>P. pentosaceus</i> crude biosurfactant for the first time, which may be explored further as an alternative to antibiotics or chemically synthesized toxic antibiofilm agents.Mohd AdnanArif Jamal SiddiquiWalid Sabri HamadouSyed Amir AshrafMd Imtaiyaz HassanMejdi SnoussiRiadh BadraouiArshad JamalFevzi BardakciAmir Mahgoub AwadelkareemManojkumar SachidanandanMitesh PatelMDPI AGarticlebiosurfactants<i>Pediococcus pentosaceus</i>antibiofilmanti-adhesionantimicrobialprobioticTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1371, p 1371 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biosurfactants <i>Pediococcus pentosaceus</i> antibiofilm anti-adhesion antimicrobial probiotic Therapeutics. Pharmacology RM1-950
spellingShingle	biosurfactants <i>Pediococcus pentosaceus</i> antibiofilm anti-adhesion antimicrobial probiotic Therapeutics. Pharmacology RM1-950 Mohd Adnan Arif Jamal Siddiqui Walid Sabri Hamadou Syed Amir Ashraf Md Imtaiyaz Hassan Mejdi Snoussi Riadh Badraoui Arshad Jamal Fevzi Bardakci Amir Mahgoub Awadelkareem Manojkumar Sachidanandan Mitesh Patel Functional and Structural Characterization of <i>Pediococcus pentosaceus</i>-Derived Biosurfactant and Its Biomedical Potential against Bacterial Adhesion, Quorum Sensing, and Biofilm Formation
description	Biosurfactants are surface-active molecules of microbial origin and alternatives to synthetic surfactants with various applications. Due to their environmental-friendliness, biocompatibility, biodegradability, effectiveness to work under various environmental conditions, and non-toxic nature, they have been recently recognized as potential agents with therapeutic and commercial importance. The biosurfactant produced by various probiotic lactic acid bacteria (LAB) has enormous applications in different fields. Thus, in vitro assessment of biofilm development prevention or disruption by natural biosurfactants derived from probiotic LAB is a plausible approach that can lead to the discovery of novel antimicrobials. Primarily, this study aims to isolate, screen, and characterize the functional and biomedical potential of biosurfactant synthesized by probiotic LAB <i>Pediococcus pentosaceus</i> (<i>P. pentosaceus</i>). Characterization consists of the assessment of critical micelle concentration (CMC), reduction in surface tension, and emulsification index (% EI24). Evaluation of antibacterial, antibiofilm, anti-QS, and anti-adhesive activities of cell-bound biosurfactants were carried out against different human pathogenic bacteria (<i>B. subtilis</i>, <i>P. aeruginosa</i>, <i>S. aureus</i>, and <i>E. coli</i>). Moreover, bacterial cell damage, viability of cells within the biofilm, and exopolysaccharide (EPS) production were also evaluated. As a result, <i>P. pentosaceus</i> was found to produce 4.75 ± 0.17 g/L biosurfactant, which displayed a CMC of 2.4 ± 0.68 g/L and reduced the surface tension from 71.11 ± 1.12 mN/m to 38.18 ± 0.58 mN/m. <i>P. pentosaceus</i> cells bound to the crude biosurfactant were found to be effective against all tested bacterial pathogens. It exhibited an anti-adhesion ability and impeded the architecture of the biofilm matrix by affecting the viability and integrity of bacterial cells within biofilms and reducing the total EPS content. Furthermore, the crude biosurfactant derived from <i>P. pentosaceus</i> was structurally characterized as a lipoprotein by GC-MS analysis, which confirms the presence of lipids and proteins. Thus, our findings represent the potent anti-adhesion and antibiofilm potential of <i>P. pentosaceus</i> crude biosurfactant for the first time, which may be explored further as an alternative to antibiotics or chemically synthesized toxic antibiofilm agents.
format	article
author	Mohd Adnan Arif Jamal Siddiqui Walid Sabri Hamadou Syed Amir Ashraf Md Imtaiyaz Hassan Mejdi Snoussi Riadh Badraoui Arshad Jamal Fevzi Bardakci Amir Mahgoub Awadelkareem Manojkumar Sachidanandan Mitesh Patel
author_facet	Mohd Adnan Arif Jamal Siddiqui Walid Sabri Hamadou Syed Amir Ashraf Md Imtaiyaz Hassan Mejdi Snoussi Riadh Badraoui Arshad Jamal Fevzi Bardakci Amir Mahgoub Awadelkareem Manojkumar Sachidanandan Mitesh Patel
author_sort	Mohd Adnan
title	Functional and Structural Characterization of <i>Pediococcus pentosaceus</i>-Derived Biosurfactant and Its Biomedical Potential against Bacterial Adhesion, Quorum Sensing, and Biofilm Formation
title_short	Functional and Structural Characterization of <i>Pediococcus pentosaceus</i>-Derived Biosurfactant and Its Biomedical Potential against Bacterial Adhesion, Quorum Sensing, and Biofilm Formation
title_full	Functional and Structural Characterization of <i>Pediococcus pentosaceus</i>-Derived Biosurfactant and Its Biomedical Potential against Bacterial Adhesion, Quorum Sensing, and Biofilm Formation
title_fullStr	Functional and Structural Characterization of <i>Pediococcus pentosaceus</i>-Derived Biosurfactant and Its Biomedical Potential against Bacterial Adhesion, Quorum Sensing, and Biofilm Formation
title_full_unstemmed	Functional and Structural Characterization of <i>Pediococcus pentosaceus</i>-Derived Biosurfactant and Its Biomedical Potential against Bacterial Adhesion, Quorum Sensing, and Biofilm Formation
title_sort	functional and structural characterization of <i>pediococcus pentosaceus</i>-derived biosurfactant and its biomedical potential against bacterial adhesion, quorum sensing, and biofilm formation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/24c3f463d4854f4fb48291aa59242da2
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Functional and Structural Characterization of <i>Pediococcus pentosaceus</i>-Derived Biosurfactant and Its Biomedical Potential against Bacterial Adhesion, Quorum Sensing, and Biofilm Formation

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