Experimental Co-infection of Low Pathogenic Avian Influenza Virus (H9N2) and Escherichia Coli in SPF Broiler Chickens.

The work was implemented to evaluate the pathogenicity of H9N2 when co-infected with the E.coli. Avian influenza H9N2 virus strain A/chicken/Egypt/1618F/2016 which was isolated from Luxor province during 2016 and E.coli serotype O78 were used in this research. Sixty SPF birds were divided into 6...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mohamed Taha, Ahmed Ibrahim, Nabila Osmsn, Ahmed Gaber, Soad Nasef, Mohammed S. Ahmed
Formato:	article
Lenguaje:	EN
Publicado:	South Valley University 2019
Materias:	co-infection e.coli lpai (h9n2) pathogenicity spf Agriculture S Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/24c63b7dfacc433dbe488cd0bd4c9926
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:24c63b7dfacc433dbe488cd0bd4c9926
record_format	dspace
spelling	oai:doaj.org-article:24c63b7dfacc433dbe488cd0bd4c99262021-12-02T10:34:50ZExperimental Co-infection of Low Pathogenic Avian Influenza Virus (H9N2) and Escherichia Coli in SPF Broiler Chickens.10.21608/SVU.2019.13393.10162535-18262535-1877https://doaj.org/article/24c63b7dfacc433dbe488cd0bd4c99262019-07-01T00:00:00Zhttps://svu.journals.ekb.eg/article_43980.htmlhttps://doaj.org/toc/2535-1826https://doaj.org/toc/2535-1877The work was implemented to evaluate the pathogenicity of H9N2 when co-infected with the E.coli. Avian influenza H9N2 virus strain A/chicken/Egypt/1618F/2016 which was isolated from Luxor province during 2016 and E.coli serotype O78 were used in this research. Sixty SPF birds were divided into 6 groups of ten birds each. At age 21 days, group 1 was inoculated with H9N2 only, group 2 received E.coli and three days later received H9N2, group 3 inoculated with H9N2 and after three days received E.coli, group 4 was challenged with E.coli and H9N2 at the same time, group 5 was inoculated with E.coli only, and group 6 was designed as a negative group. Clinical signs, postmortem examination, and serological examination were monitored for 15 days. The H9N2 virus presence was assessed in tracheal and cloacal swabs using real-time PCR. The most severe signs and lesions were observed in groups (3, and 4), with also high mortality rate than other groups with a percentage of 20%. Groups 3, and 4 showed a longer duration in virus shedding in the tracheal and cloacal samples. The hemagglutination inhibition test, group 4 showed high HI antibody titer against AIV-H9N2 antigen than other groups, while group 2 showed the lowest HI antibody titer against AIV-H9N2 antigen than other groups that received only the H9N2 virus. In conclusion, the outcomes of this study revealed that the infection of H9N2 with E.coli can exacerbate the clinical outcomes and mortality rates which leads to higher economic losses in chicken flocks. Mohamed TahaAhmed IbrahimNabila OsmsnAhmed GaberSoad NasefMohammed S. AhmedSouth Valley Universityarticleco-infectione.colilpai (h9n2)pathogenicityspfAgricultureSVeterinary medicineSF600-1100ENSVU-International Journal of Veterinary Sciences, Vol 2, Iss 2, Pp 91-100 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	co-infection e.coli lpai (h9n2) pathogenicity spf Agriculture S Veterinary medicine SF600-1100
spellingShingle	co-infection e.coli lpai (h9n2) pathogenicity spf Agriculture S Veterinary medicine SF600-1100 Mohamed Taha Ahmed Ibrahim Nabila Osmsn Ahmed Gaber Soad Nasef Mohammed S. Ahmed Experimental Co-infection of Low Pathogenic Avian Influenza Virus (H9N2) and Escherichia Coli in SPF Broiler Chickens.
description	The work was implemented to evaluate the pathogenicity of H9N2 when co-infected with the E.coli. Avian influenza H9N2 virus strain A/chicken/Egypt/1618F/2016 which was isolated from Luxor province during 2016 and E.coli serotype O78 were used in this research. Sixty SPF birds were divided into 6 groups of ten birds each. At age 21 days, group 1 was inoculated with H9N2 only, group 2 received E.coli and three days later received H9N2, group 3 inoculated with H9N2 and after three days received E.coli, group 4 was challenged with E.coli and H9N2 at the same time, group 5 was inoculated with E.coli only, and group 6 was designed as a negative group. Clinical signs, postmortem examination, and serological examination were monitored for 15 days. The H9N2 virus presence was assessed in tracheal and cloacal swabs using real-time PCR. The most severe signs and lesions were observed in groups (3, and 4), with also high mortality rate than other groups with a percentage of 20%. Groups 3, and 4 showed a longer duration in virus shedding in the tracheal and cloacal samples. The hemagglutination inhibition test, group 4 showed high HI antibody titer against AIV-H9N2 antigen than other groups, while group 2 showed the lowest HI antibody titer against AIV-H9N2 antigen than other groups that received only the H9N2 virus. In conclusion, the outcomes of this study revealed that the infection of H9N2 with E.coli can exacerbate the clinical outcomes and mortality rates which leads to higher economic losses in chicken flocks.
format	article
author	Mohamed Taha Ahmed Ibrahim Nabila Osmsn Ahmed Gaber Soad Nasef Mohammed S. Ahmed
author_facet	Mohamed Taha Ahmed Ibrahim Nabila Osmsn Ahmed Gaber Soad Nasef Mohammed S. Ahmed
author_sort	Mohamed Taha
title	Experimental Co-infection of Low Pathogenic Avian Influenza Virus (H9N2) and Escherichia Coli in SPF Broiler Chickens.
title_short	Experimental Co-infection of Low Pathogenic Avian Influenza Virus (H9N2) and Escherichia Coli in SPF Broiler Chickens.
title_full	Experimental Co-infection of Low Pathogenic Avian Influenza Virus (H9N2) and Escherichia Coli in SPF Broiler Chickens.
title_fullStr	Experimental Co-infection of Low Pathogenic Avian Influenza Virus (H9N2) and Escherichia Coli in SPF Broiler Chickens.
title_full_unstemmed	Experimental Co-infection of Low Pathogenic Avian Influenza Virus (H9N2) and Escherichia Coli in SPF Broiler Chickens.
title_sort	experimental co-infection of low pathogenic avian influenza virus (h9n2) and escherichia coli in spf broiler chickens.
publisher	South Valley University
publishDate	2019
url	https://doaj.org/article/24c63b7dfacc433dbe488cd0bd4c9926
work_keys_str_mv	AT mohamedtaha experimentalcoinfectionoflowpathogenicavianinfluenzavirush9n2andescherichiacoliinspfbroilerchickens AT ahmedibrahim experimentalcoinfectionoflowpathogenicavianinfluenzavirush9n2andescherichiacoliinspfbroilerchickens AT nabilaosmsn experimentalcoinfectionoflowpathogenicavianinfluenzavirush9n2andescherichiacoliinspfbroilerchickens AT ahmedgaber experimentalcoinfectionoflowpathogenicavianinfluenzavirush9n2andescherichiacoliinspfbroilerchickens AT soadnasef experimentalcoinfectionoflowpathogenicavianinfluenzavirush9n2andescherichiacoliinspfbroilerchickens AT mohammedsahmed experimentalcoinfectionoflowpathogenicavianinfluenzavirush9n2andescherichiacoliinspfbroilerchickens
_version_	1718396987572748288

Experimental Co-infection of Low Pathogenic Avian Influenza Virus (H9N2) and Escherichia Coli in SPF Broiler Chickens.

Ejemplares similares