Short Communication: DNA extraction from stored wood of Falcataria moluccana suitable for barcoding analysis

Abstract. Shabrina H, Siregar UJ, Matra DD, Kamiya D, Siregar IZ. 2019. Short Communication: DNA extraction from stored wood of Falcataria moluccana suitable for barcoding analysis. Biodiversitas 20: 1748-1752. Sengon or Falcataria moluccana (Miq.) Barneby & J.W.Grimes is the main crop in commun...

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Autores principales:	HASYYATI SHABRINA, ULFAH J SIREGAR, DEDEN D MATRA, KOICHI KAMIYA, ISKANDAR Z SIREGAR
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	barcode, wood dna isolation, sengon, liquid nitrogen Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/24c731658161434c96368ed7b7c3886c
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spelling	oai:doaj.org-article:24c731658161434c96368ed7b7c3886c2021-11-21T21:41:27ZShort Communication: DNA extraction from stored wood of Falcataria moluccana suitable for barcoding analysis1412-033X2085-472210.13057/biodiv/d200635https://doaj.org/article/24c731658161434c96368ed7b7c3886c2019-05-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3879https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Shabrina H, Siregar UJ, Matra DD, Kamiya D, Siregar IZ. 2019. Short Communication: DNA extraction from stored wood of Falcataria moluccana suitable for barcoding analysis. Biodiversitas 20: 1748-1752. Sengon or Falcataria moluccana (Miq.) Barneby & J.W.Grimes is the main crop in community tree plantations in Java, Indonesia, favored because of its fast-growing property and the promising economic return. The wood itself is mostly used for light construction, furniture, plywood, packing materials, and recently as feedstock for bioenergy. Sometimes the wood is used as DNA source for example in the wood identification analysis. However, extracting DNA from woods is considered difficult due particularly to very small quantities of DNA. The objective of this research was to optimize and modify the common CTAB protocols to extract DNA from Sengon wood without liquid nitrogen which sometimes unavailable in some laboratories. The extracted DNA was quantified with nanophotometer and gel visualization and amplified with primer coding psbA-trnH intergenic spacer for testing. The highest concentration of DNA extracted was from 100 mg of wood stored for 24 hours in -30 °C (257.80 ng/µL or 14.18 µg in total) and even the lowest concentration produced by this method able to produce sufficient amount of PCR product for sequencing. Compared with results from 200 mg samples and longer freezing time (72 hours) and extraction using liquid nitrogen, this method considered gave the best results.HASYYATI SHABRINAULFAH J SIREGARDEDEN D MATRAKOICHI KAMIYAISKANDAR Z SIREGARMBI & UNS Soloarticlebarcode, wood dna isolation, sengon, liquid nitrogenBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 6 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	barcode, wood dna isolation, sengon, liquid nitrogen Biology (General) QH301-705.5
spellingShingle	barcode, wood dna isolation, sengon, liquid nitrogen Biology (General) QH301-705.5 HASYYATI SHABRINA ULFAH J SIREGAR DEDEN D MATRA KOICHI KAMIYA ISKANDAR Z SIREGAR Short Communication: DNA extraction from stored wood of Falcataria moluccana suitable for barcoding analysis
description	Abstract. Shabrina H, Siregar UJ, Matra DD, Kamiya D, Siregar IZ. 2019. Short Communication: DNA extraction from stored wood of Falcataria moluccana suitable for barcoding analysis. Biodiversitas 20: 1748-1752. Sengon or Falcataria moluccana (Miq.) Barneby & J.W.Grimes is the main crop in community tree plantations in Java, Indonesia, favored because of its fast-growing property and the promising economic return. The wood itself is mostly used for light construction, furniture, plywood, packing materials, and recently as feedstock for bioenergy. Sometimes the wood is used as DNA source for example in the wood identification analysis. However, extracting DNA from woods is considered difficult due particularly to very small quantities of DNA. The objective of this research was to optimize and modify the common CTAB protocols to extract DNA from Sengon wood without liquid nitrogen which sometimes unavailable in some laboratories. The extracted DNA was quantified with nanophotometer and gel visualization and amplified with primer coding psbA-trnH intergenic spacer for testing. The highest concentration of DNA extracted was from 100 mg of wood stored for 24 hours in -30 °C (257.80 ng/µL or 14.18 µg in total) and even the lowest concentration produced by this method able to produce sufficient amount of PCR product for sequencing. Compared with results from 200 mg samples and longer freezing time (72 hours) and extraction using liquid nitrogen, this method considered gave the best results.
format	article
author	HASYYATI SHABRINA ULFAH J SIREGAR DEDEN D MATRA KOICHI KAMIYA ISKANDAR Z SIREGAR
author_facet	HASYYATI SHABRINA ULFAH J SIREGAR DEDEN D MATRA KOICHI KAMIYA ISKANDAR Z SIREGAR
author_sort	HASYYATI SHABRINA
title	Short Communication: DNA extraction from stored wood of Falcataria moluccana suitable for barcoding analysis
title_short	Short Communication: DNA extraction from stored wood of Falcataria moluccana suitable for barcoding analysis
title_full	Short Communication: DNA extraction from stored wood of Falcataria moluccana suitable for barcoding analysis
title_fullStr	Short Communication: DNA extraction from stored wood of Falcataria moluccana suitable for barcoding analysis
title_full_unstemmed	Short Communication: DNA extraction from stored wood of Falcataria moluccana suitable for barcoding analysis
title_sort	short communication: dna extraction from stored wood of falcataria moluccana suitable for barcoding analysis
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/24c731658161434c96368ed7b7c3886c
work_keys_str_mv	AT hasyyatishabrina shortcommunicationdnaextractionfromstoredwoodoffalcatariamoluccanasuitableforbarcodinganalysis AT ulfahjsiregar shortcommunicationdnaextractionfromstoredwoodoffalcatariamoluccanasuitableforbarcodinganalysis AT dedendmatra shortcommunicationdnaextractionfromstoredwoodoffalcatariamoluccanasuitableforbarcodinganalysis AT koichikamiya shortcommunicationdnaextractionfromstoredwoodoffalcatariamoluccanasuitableforbarcodinganalysis AT iskandarzsiregar shortcommunicationdnaextractionfromstoredwoodoffalcatariamoluccanasuitableforbarcodinganalysis
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Short Communication: DNA extraction from stored wood of Falcataria moluccana suitable for barcoding analysis

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