Light perception in aerial tissues enhances DWF4 accumulation in root tips and induces root growth

Abstract Many attempts have been made to characterize the activities of brassinosteroids (BRs), which are important plant hormones. The crosstalk between light perception and the BR signalling pathway has been extensively studied regarding its effects on photomorphogenesis, especially in elongating...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jun Sakaguchi, Yuichiro Watanabe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/24c85da9d4f840e79ccdd8598ebdc3d6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:24c85da9d4f840e79ccdd8598ebdc3d6
record_format	dspace
spelling	oai:doaj.org-article:24c85da9d4f840e79ccdd8598ebdc3d62021-12-02T16:06:01ZLight perception in aerial tissues enhances DWF4 accumulation in root tips and induces root growth10.1038/s41598-017-01872-42045-2322https://doaj.org/article/24c85da9d4f840e79ccdd8598ebdc3d62017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01872-4https://doaj.org/toc/2045-2322Abstract Many attempts have been made to characterize the activities of brassinosteroids (BRs), which are important plant hormones. The crosstalk between light perception and the BR signalling pathway has been extensively studied regarding its effects on photomorphogenesis, especially in elongating etiolated hypocotyls. In contrast, how and where the light induces BR biosynthesis remain uncharacterized. DWF4 is one of the main enzymes involved in the BR biosynthesis pathway in Arabidopsis thaliana. We established DWF4-GUS A. thaliana lines in a homozygous dwf4-102 genetic background, but functionally complemented with a genomic DWF4 sequence fused in-frame with a β-glucuronidase (GUS) marker gene. The DWF4-GUS plants enabled the visualization of the accumulation of DWF4 under different conditions. We investigated the effects of aboveground light on root and hypocotyl growth. We observed that root length increased when shoots were maintained under light irrespective of whether roots were exposed to light. We also determined that light perception in aerial tissues enhanced DWF4 accumulation in the root tips. Overall, our data indicate that BR biosynthesis is promoted in the root tip regions by an unknown mechanism in distantly located shoot tissues exposed to light, leading to increased root growth.Jun SakaguchiYuichiro WatanabeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jun Sakaguchi Yuichiro Watanabe Light perception in aerial tissues enhances DWF4 accumulation in root tips and induces root growth
description	Abstract Many attempts have been made to characterize the activities of brassinosteroids (BRs), which are important plant hormones. The crosstalk between light perception and the BR signalling pathway has been extensively studied regarding its effects on photomorphogenesis, especially in elongating etiolated hypocotyls. In contrast, how and where the light induces BR biosynthesis remain uncharacterized. DWF4 is one of the main enzymes involved in the BR biosynthesis pathway in Arabidopsis thaliana. We established DWF4-GUS A. thaliana lines in a homozygous dwf4-102 genetic background, but functionally complemented with a genomic DWF4 sequence fused in-frame with a β-glucuronidase (GUS) marker gene. The DWF4-GUS plants enabled the visualization of the accumulation of DWF4 under different conditions. We investigated the effects of aboveground light on root and hypocotyl growth. We observed that root length increased when shoots were maintained under light irrespective of whether roots were exposed to light. We also determined that light perception in aerial tissues enhanced DWF4 accumulation in the root tips. Overall, our data indicate that BR biosynthesis is promoted in the root tip regions by an unknown mechanism in distantly located shoot tissues exposed to light, leading to increased root growth.
format	article
author	Jun Sakaguchi Yuichiro Watanabe
author_facet	Jun Sakaguchi Yuichiro Watanabe
author_sort	Jun Sakaguchi
title	Light perception in aerial tissues enhances DWF4 accumulation in root tips and induces root growth
title_short	Light perception in aerial tissues enhances DWF4 accumulation in root tips and induces root growth
title_full	Light perception in aerial tissues enhances DWF4 accumulation in root tips and induces root growth
title_fullStr	Light perception in aerial tissues enhances DWF4 accumulation in root tips and induces root growth
title_full_unstemmed	Light perception in aerial tissues enhances DWF4 accumulation in root tips and induces root growth
title_sort	light perception in aerial tissues enhances dwf4 accumulation in root tips and induces root growth
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/24c85da9d4f840e79ccdd8598ebdc3d6
work_keys_str_mv	AT junsakaguchi lightperceptioninaerialtissuesenhancesdwf4accumulationinroottipsandinducesrootgrowth AT yuichirowatanabe lightperceptioninaerialtissuesenhancesdwf4accumulationinroottipsandinducesrootgrowth
_version_	1718385135370371072

Light perception in aerial tissues enhances DWF4 accumulation in root tips and induces root growth

Ejemplares similares