Establishment of a novel hepatitis B virus culture system using immortalized human hepatocytes

Abstract Recent development of hepatitis B virus (HBV) culture systems has made it possible to analyze the almost all steps of the viral life cycle. However, the reproducibility of interaction between HBV and host cells seemed inaccurate in those systems because of utilization of cancer cell lines w...

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Autores principales:	Yuichi Akahori, Hiroki Kato, Takashi Fujita, Kohji Moriishi, Yasuhito Tanaka, Koichi Watashi, Michio Imamura, Kazuaki Chayama, Takaji Wakita, Makoto Hijikata
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/24c87ff8e1ec41cd8e076d743a800587
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spelling	oai:doaj.org-article:24c87ff8e1ec41cd8e076d743a8005872021-12-02T15:11:49ZEstablishment of a novel hepatitis B virus culture system using immortalized human hepatocytes10.1038/s41598-020-78655-x2045-2322https://doaj.org/article/24c87ff8e1ec41cd8e076d743a8005872020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78655-xhttps://doaj.org/toc/2045-2322Abstract Recent development of hepatitis B virus (HBV) culture systems has made it possible to analyze the almost all steps of the viral life cycle. However, the reproducibility of interaction between HBV and host cells seemed inaccurate in those systems because of utilization of cancer cell lines with a difference from hepatocytes in the majority of cases. In this study, in order to resolve this point, a novel HBV culture system using non-cancer-derived immortalized human hepatocytes derived cell lines, producing exogenous human sodium taurocholate cotransporting polypeptide, was developed. One of the cell clones, E/NtG8 cells, was permissive to both blood-borne HBV (HBVbb) and culture-derived recombinant HBV when cultured in the three-dimensional condition. Furthermore, the production of infectious HBV particles, which showed the similar physicochemical properties to HBVbb, was observed for about a month after HBVbb infection in this system, suggesting that it may reproduce whole steps of the HBV lifecycle under the condition analogous to human liver cells infected with HBV. This system seemed to contribute not only to find novel interactions between HBV and host cells but also to understand mechanism of HBV pathogenesis.Yuichi AkahoriHiroki KatoTakashi FujitaKohji MoriishiYasuhito TanakaKoichi WatashiMichio ImamuraKazuaki ChayamaTakaji WakitaMakoto HijikataNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuichi Akahori Hiroki Kato Takashi Fujita Kohji Moriishi Yasuhito Tanaka Koichi Watashi Michio Imamura Kazuaki Chayama Takaji Wakita Makoto Hijikata Establishment of a novel hepatitis B virus culture system using immortalized human hepatocytes
description	Abstract Recent development of hepatitis B virus (HBV) culture systems has made it possible to analyze the almost all steps of the viral life cycle. However, the reproducibility of interaction between HBV and host cells seemed inaccurate in those systems because of utilization of cancer cell lines with a difference from hepatocytes in the majority of cases. In this study, in order to resolve this point, a novel HBV culture system using non-cancer-derived immortalized human hepatocytes derived cell lines, producing exogenous human sodium taurocholate cotransporting polypeptide, was developed. One of the cell clones, E/NtG8 cells, was permissive to both blood-borne HBV (HBVbb) and culture-derived recombinant HBV when cultured in the three-dimensional condition. Furthermore, the production of infectious HBV particles, which showed the similar physicochemical properties to HBVbb, was observed for about a month after HBVbb infection in this system, suggesting that it may reproduce whole steps of the HBV lifecycle under the condition analogous to human liver cells infected with HBV. This system seemed to contribute not only to find novel interactions between HBV and host cells but also to understand mechanism of HBV pathogenesis.
format	article
author	Yuichi Akahori Hiroki Kato Takashi Fujita Kohji Moriishi Yasuhito Tanaka Koichi Watashi Michio Imamura Kazuaki Chayama Takaji Wakita Makoto Hijikata
author_facet	Yuichi Akahori Hiroki Kato Takashi Fujita Kohji Moriishi Yasuhito Tanaka Koichi Watashi Michio Imamura Kazuaki Chayama Takaji Wakita Makoto Hijikata
author_sort	Yuichi Akahori
title	Establishment of a novel hepatitis B virus culture system using immortalized human hepatocytes
title_short	Establishment of a novel hepatitis B virus culture system using immortalized human hepatocytes
title_full	Establishment of a novel hepatitis B virus culture system using immortalized human hepatocytes
title_fullStr	Establishment of a novel hepatitis B virus culture system using immortalized human hepatocytes
title_full_unstemmed	Establishment of a novel hepatitis B virus culture system using immortalized human hepatocytes
title_sort	establishment of a novel hepatitis b virus culture system using immortalized human hepatocytes
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/24c87ff8e1ec41cd8e076d743a800587
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_version_	1718387618003025920

Establishment of a novel hepatitis B virus culture system using immortalized human hepatocytes

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