Transferrin coated nanoparticles: study of the bionano interface in human plasma.

It is now well established that the surface of nanoparticles (NPs) in a biological environment is immediately modified by the adsorption of biomolecules with the formation of a protein corona and it is also accepted that the protein corona, rather than the original nanoparticle surface, defines a ne...

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Autores principales:	Andrzej S Pitek, David O'Connell, Eugene Mahon, Marco P Monopoli, Francesca Baldelli Bombelli, Kenneth A Dawson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/24ca73850bad4e08aa791c74f372c992
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spelling	oai:doaj.org-article:24ca73850bad4e08aa791c74f372c9922021-11-18T07:11:54ZTransferrin coated nanoparticles: study of the bionano interface in human plasma.1932-620310.1371/journal.pone.0040685https://doaj.org/article/24ca73850bad4e08aa791c74f372c9922012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22829881/?tool=EBIhttps://doaj.org/toc/1932-6203It is now well established that the surface of nanoparticles (NPs) in a biological environment is immediately modified by the adsorption of biomolecules with the formation of a protein corona and it is also accepted that the protein corona, rather than the original nanoparticle surface, defines a new biological identity. Consequently, a methodology to effectively study the interaction between nanomaterials and the biological corona encountered within an organism is a key objective in nanoscience for understanding the impact of the nanoparticle-protein interactions on the biological response in vitro and in vivo. Here, we outline an integrated methodology to address the different aspects governing the formation and the function of the protein corona of polystyrene nanoparticles coated with Transferrin by different strategies. Protein-NP complexes are studied both in situ (in human plasma, full corona FC) and after washing (hard corona, HC) in terms of structural properties, composition and second-order interactions with protein microarrays. Human protein microarrays are used to effectively study NP-corona/proteins interactions addressing the growing demand to advance investigations of the extrinsic function of corona complexes. Our data highlight the importance of this methodology as an analysis to be used in advance of the application of engineered NPs in biological environments.Andrzej S PitekDavid O'ConnellEugene MahonMarco P MonopoliFrancesca Baldelli BombelliKenneth A DawsonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e40685 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andrzej S Pitek David O'Connell Eugene Mahon Marco P Monopoli Francesca Baldelli Bombelli Kenneth A Dawson Transferrin coated nanoparticles: study of the bionano interface in human plasma.
description	It is now well established that the surface of nanoparticles (NPs) in a biological environment is immediately modified by the adsorption of biomolecules with the formation of a protein corona and it is also accepted that the protein corona, rather than the original nanoparticle surface, defines a new biological identity. Consequently, a methodology to effectively study the interaction between nanomaterials and the biological corona encountered within an organism is a key objective in nanoscience for understanding the impact of the nanoparticle-protein interactions on the biological response in vitro and in vivo. Here, we outline an integrated methodology to address the different aspects governing the formation and the function of the protein corona of polystyrene nanoparticles coated with Transferrin by different strategies. Protein-NP complexes are studied both in situ (in human plasma, full corona FC) and after washing (hard corona, HC) in terms of structural properties, composition and second-order interactions with protein microarrays. Human protein microarrays are used to effectively study NP-corona/proteins interactions addressing the growing demand to advance investigations of the extrinsic function of corona complexes. Our data highlight the importance of this methodology as an analysis to be used in advance of the application of engineered NPs in biological environments.
format	article
author	Andrzej S Pitek David O'Connell Eugene Mahon Marco P Monopoli Francesca Baldelli Bombelli Kenneth A Dawson
author_facet	Andrzej S Pitek David O'Connell Eugene Mahon Marco P Monopoli Francesca Baldelli Bombelli Kenneth A Dawson
author_sort	Andrzej S Pitek
title	Transferrin coated nanoparticles: study of the bionano interface in human plasma.
title_short	Transferrin coated nanoparticles: study of the bionano interface in human plasma.
title_full	Transferrin coated nanoparticles: study of the bionano interface in human plasma.
title_fullStr	Transferrin coated nanoparticles: study of the bionano interface in human plasma.
title_full_unstemmed	Transferrin coated nanoparticles: study of the bionano interface in human plasma.
title_sort	transferrin coated nanoparticles: study of the bionano interface in human plasma.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/24ca73850bad4e08aa791c74f372c992
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Transferrin coated nanoparticles: study of the bionano interface in human plasma.

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