Carbon nanotubes impregnated with subventricular zone neural progenitor cells promotes recovery from stroke

Sung Ung Moon,1,* Jihee Kim,1,2,* Kiran Kumar Bokara,1,* Jong Youl Kim,1 Dongwoo Khang,3,4 Thomas J Webster,3,4 Jong Eun Lee1,21Department of Anatomy, 2Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea; 3School of Engineering, 4Department of Orthop...

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Autores principales:	Moon SU, Kim J, Bokara KK, Kim JY, Khang D, Webster TJ, Lee JE
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/24cec00584ab445da5912361aa8bc886
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spelling	oai:doaj.org-article:24cec00584ab445da5912361aa8bc8862021-12-02T05:02:17ZCarbon nanotubes impregnated with subventricular zone neural progenitor cells promotes recovery from stroke1176-91141178-2013https://doaj.org/article/24cec00584ab445da5912361aa8bc8862012-06-01T00:00:00Zhttp://www.dovepress.com/carbon-nanotubes-impregnated-with-subventricular-zone-neural-progenito-a10020https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Sung Ung Moon,1,* Jihee Kim,1,2,* Kiran Kumar Bokara,1,* Jong Youl Kim,1 Dongwoo Khang,3,4 Thomas J Webster,3,4 Jong Eun Lee1,21Department of Anatomy, 2Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea; 3School of Engineering, 4Department of Orthopedics, Brown University, Providence, RI, USA*These authors contributed equally to this workAbstract: The present in vivo study was conducted to evaluate whether hydrophilic (HL) or hydrophobic (HP) carbon nanotubes (CNTs) impregnated with subventricular zone neural progenitor cells (SVZ NPCs) could repair damaged neural tissue following stroke. For this purpose, stroke damaged rats were transplanted with HL CNT-SVZ NPCs, HP CNT-SVZ NPCs, or SVZ NPCs alone for 1, 3, 5, and 8 weeks. Results showed that the HP CNT-SVZ NPC transplants improved rat behavior and reduced infarct cyst volume and infarct cyst area compared with the experimental control and the HL CNT-SVZ NPC and SVZ NPCs alone groups. The transplantation groups showed an increase in the expression of nestin (cell stemness marker) and proliferation which was evident with the increased number of doublecortin and bromodeoxyuridine double-stained immunopositive cells around the lesion site. But, these effects were more prominent in the HP CNT-SVZ NPC group compared with the other transplantation groups. The HP CNT-SVZ NPC and HL CNT-SVZ NPC transplants increased the number of microtubule-associated protein 2 (marker for neurons) and decreased the number of glial fibrillary acidic protein (marker for astroglial cells) positive cells within the injury epicenter. The majority of the transplanted HP CNT-SVZ NPCs collectively broadened around the ischemic injured region and the SVZ NPCs differentiated into mature neurons, attained the synapse morphology (TUJ1, synaptophysin), and decreased microglial activation (CD11b/c [OX-42]). For these reasons, this study provided the first evidence that CNTs can improve stem cell differentiation to heal stroke damage and, thus, deserve further attention.Keywords: biocompatibility, brain, in vivo, stem cell, wettability, carbon nanotubes, strokeMoon SUKim JBokara KKKim JYKhang DWebster TJLee JEDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 2751-2765 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Moon SU Kim J Bokara KK Kim JY Khang D Webster TJ Lee JE Carbon nanotubes impregnated with subventricular zone neural progenitor cells promotes recovery from stroke
description	Sung Ung Moon,1,* Jihee Kim,1,2,* Kiran Kumar Bokara,1,* Jong Youl Kim,1 Dongwoo Khang,3,4 Thomas J Webster,3,4 Jong Eun Lee1,21Department of Anatomy, 2Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea; 3School of Engineering, 4Department of Orthopedics, Brown University, Providence, RI, USA*These authors contributed equally to this workAbstract: The present in vivo study was conducted to evaluate whether hydrophilic (HL) or hydrophobic (HP) carbon nanotubes (CNTs) impregnated with subventricular zone neural progenitor cells (SVZ NPCs) could repair damaged neural tissue following stroke. For this purpose, stroke damaged rats were transplanted with HL CNT-SVZ NPCs, HP CNT-SVZ NPCs, or SVZ NPCs alone for 1, 3, 5, and 8 weeks. Results showed that the HP CNT-SVZ NPC transplants improved rat behavior and reduced infarct cyst volume and infarct cyst area compared with the experimental control and the HL CNT-SVZ NPC and SVZ NPCs alone groups. The transplantation groups showed an increase in the expression of nestin (cell stemness marker) and proliferation which was evident with the increased number of doublecortin and bromodeoxyuridine double-stained immunopositive cells around the lesion site. But, these effects were more prominent in the HP CNT-SVZ NPC group compared with the other transplantation groups. The HP CNT-SVZ NPC and HL CNT-SVZ NPC transplants increased the number of microtubule-associated protein 2 (marker for neurons) and decreased the number of glial fibrillary acidic protein (marker for astroglial cells) positive cells within the injury epicenter. The majority of the transplanted HP CNT-SVZ NPCs collectively broadened around the ischemic injured region and the SVZ NPCs differentiated into mature neurons, attained the synapse morphology (TUJ1, synaptophysin), and decreased microglial activation (CD11b/c [OX-42]). For these reasons, this study provided the first evidence that CNTs can improve stem cell differentiation to heal stroke damage and, thus, deserve further attention.Keywords: biocompatibility, brain, in vivo, stem cell, wettability, carbon nanotubes, stroke
format	article
author	Moon SU Kim J Bokara KK Kim JY Khang D Webster TJ Lee JE
author_facet	Moon SU Kim J Bokara KK Kim JY Khang D Webster TJ Lee JE
author_sort	Moon SU
title	Carbon nanotubes impregnated with subventricular zone neural progenitor cells promotes recovery from stroke
title_short	Carbon nanotubes impregnated with subventricular zone neural progenitor cells promotes recovery from stroke
title_full	Carbon nanotubes impregnated with subventricular zone neural progenitor cells promotes recovery from stroke
title_fullStr	Carbon nanotubes impregnated with subventricular zone neural progenitor cells promotes recovery from stroke
title_full_unstemmed	Carbon nanotubes impregnated with subventricular zone neural progenitor cells promotes recovery from stroke
title_sort	carbon nanotubes impregnated with subventricular zone neural progenitor cells promotes recovery from stroke
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/24cec00584ab445da5912361aa8bc886
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_version_	1718400757793816576

Carbon nanotubes impregnated with subventricular zone neural progenitor cells promotes recovery from stroke

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