Nano rough micron patterned titanium for directing osteoblast morphology and adhesion

Sabrina Puckett, Rajesh Pareta, Thomas J WebsterDivision of Engineering, Brown University, Providence, RI, USAAbstract: Previous studies have demonstrated greater functions of osteoblasts (bone-forming cells) on nanophase compared with conventional metals. Nanophase metals possess a biologically ins...

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Autores principales:	Sabrina Puckett, Rajesh Pareta, Thomas J Webster
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2008
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/24ceeea3c1b44d5092e9a3bf949469ce
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spelling	oai:doaj.org-article:24ceeea3c1b44d5092e9a3bf949469ce2021-12-02T07:28:31ZNano rough micron patterned titanium for directing osteoblast morphology and adhesion1176-91141178-2013https://doaj.org/article/24ceeea3c1b44d5092e9a3bf949469ce2008-06-01T00:00:00Zhttp://www.dovepress.com/nano-rough-micron-patterned-titanium-for-directing-osteoblast-morpholo-a1834https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Sabrina Puckett, Rajesh Pareta, Thomas J WebsterDivision of Engineering, Brown University, Providence, RI, USAAbstract: Previous studies have demonstrated greater functions of osteoblasts (bone-forming cells) on nanophase compared with conventional metals. Nanophase metals possess a biologically inspired nanostructured surface that mimics the dimensions of constituent components in bone, including collagen and hydroxyapatite. Not only do these components possess dimensions on the nanoscale, they are aligned in a parallel manner creating a defined orientation in bone. To date, research has yet to evaluate the effect that organized nanosurface features can have on the interaction of osteoblasts with material surfaces. Therefore, to determine if surface orientation of features can mediate osteoblast adhesion and morphology, this study investigated osteoblast function on patterned titanium substrates containing alternating regions of micron rough and nano rough surfaces prepared by novel electron beam evaporation techniques. This study was also interested in determining whether or not the size of the patterned regions had an effect on osteoblast behavior and alignment. Results indicated early controlled osteoblast alignment on these patterned materials as well as greater osteoblast adhesion on the nano rough regions of these patterned substrates. Interestingly, decreasing the width of the nano rough regions (from 80 &micro;m to 22 &micro;m) on these patterned substrates resulted in a decreased number of osteoblasts adhering to these areas. Changes in the width of the nano rough regions also resulted in changes in osteoblast morphology, thus, suggesting there is an optimal pattern dimension that osteoblasts prefer. In summary, results of this study provided evidence that aligned nanophase metal features on the surface of titanium improved early osteoblast functions (morphology and adhesion) promising for their long term functions, criteria necessary to improve orthopedic implant efficacy.Keywords: osteoblasts, titanium, nanophase, orthopedic, alignment, surface topography Sabrina PuckettRajesh ParetaThomas J WebsterDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2008, Iss Issue 2, Pp 229-241 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Sabrina Puckett Rajesh Pareta Thomas J Webster Nano rough micron patterned titanium for directing osteoblast morphology and adhesion
description	Sabrina Puckett, Rajesh Pareta, Thomas J WebsterDivision of Engineering, Brown University, Providence, RI, USAAbstract: Previous studies have demonstrated greater functions of osteoblasts (bone-forming cells) on nanophase compared with conventional metals. Nanophase metals possess a biologically inspired nanostructured surface that mimics the dimensions of constituent components in bone, including collagen and hydroxyapatite. Not only do these components possess dimensions on the nanoscale, they are aligned in a parallel manner creating a defined orientation in bone. To date, research has yet to evaluate the effect that organized nanosurface features can have on the interaction of osteoblasts with material surfaces. Therefore, to determine if surface orientation of features can mediate osteoblast adhesion and morphology, this study investigated osteoblast function on patterned titanium substrates containing alternating regions of micron rough and nano rough surfaces prepared by novel electron beam evaporation techniques. This study was also interested in determining whether or not the size of the patterned regions had an effect on osteoblast behavior and alignment. Results indicated early controlled osteoblast alignment on these patterned materials as well as greater osteoblast adhesion on the nano rough regions of these patterned substrates. Interestingly, decreasing the width of the nano rough regions (from 80 &micro;m to 22 &micro;m) on these patterned substrates resulted in a decreased number of osteoblasts adhering to these areas. Changes in the width of the nano rough regions also resulted in changes in osteoblast morphology, thus, suggesting there is an optimal pattern dimension that osteoblasts prefer. In summary, results of this study provided evidence that aligned nanophase metal features on the surface of titanium improved early osteoblast functions (morphology and adhesion) promising for their long term functions, criteria necessary to improve orthopedic implant efficacy.Keywords: osteoblasts, titanium, nanophase, orthopedic, alignment, surface topography
format	article
author	Sabrina Puckett Rajesh Pareta Thomas J Webster
author_facet	Sabrina Puckett Rajesh Pareta Thomas J Webster
author_sort	Sabrina Puckett
title	Nano rough micron patterned titanium for directing osteoblast morphology and adhesion
title_short	Nano rough micron patterned titanium for directing osteoblast morphology and adhesion
title_full	Nano rough micron patterned titanium for directing osteoblast morphology and adhesion
title_fullStr	Nano rough micron patterned titanium for directing osteoblast morphology and adhesion
title_full_unstemmed	Nano rough micron patterned titanium for directing osteoblast morphology and adhesion
title_sort	nano rough micron patterned titanium for directing osteoblast morphology and adhesion
publisher	Dove Medical Press
publishDate	2008
url	https://doaj.org/article/24ceeea3c1b44d5092e9a3bf949469ce
work_keys_str_mv	AT sabrinapuckett nanoroughmicronpatternedtitaniumfordirectingosteoblastmorphologyandadhesion AT rajeshpareta nanoroughmicronpatternedtitaniumfordirectingosteoblastmorphologyandadhesion AT thomasjwebster nanoroughmicronpatternedtitaniumfordirectingosteoblastmorphologyandadhesion
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Nano rough micron patterned titanium for directing osteoblast morphology and adhesion

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