Transcriptome analysis reveals plant response to colchicine treatment during on chromosome doubling

Abstract Colchicine was commonly used to artificially double chromosomes while the transcriptome changes in colchicine treated plants has rarely been characterized. To understand the molecular mechanism of colchicine on chromosome doubling, we characterized transcriptome data of diploid orchardgrass...

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Autores principales:	Kai Zhou, Paige Fleet, Eviatar Nevo, Xinquan Zhang, Genlou Sun
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/24cff67896ef458396b36e8b8d22af8c
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spelling	oai:doaj.org-article:24cff67896ef458396b36e8b8d22af8c2021-12-02T15:05:06ZTranscriptome analysis reveals plant response to colchicine treatment during on chromosome doubling10.1038/s41598-017-08391-22045-2322https://doaj.org/article/24cff67896ef458396b36e8b8d22af8c2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08391-2https://doaj.org/toc/2045-2322Abstract Colchicine was commonly used to artificially double chromosomes while the transcriptome changes in colchicine treated plants has rarely been characterized. To understand the molecular mechanism of colchicine on chromosome doubling, we characterized transcriptome data of diploid orchardgrass root after colchicine treatment. Our results showed that 3381 of differentially expressed genes (DEGs) were mainly affected by water stress, 1258 DEGs that were expressed significantly in sample DacR5tr but not in DacR5ck were considered to be mainly affected by colchicine and combination of water and colchicine. These DEGs mainly regulated by colchicine were enriched to gene ontology (GO) accessions of cation binding, catalytic activity, membrane and transporter activity, and enriched to Kyoto Encyclopedia of Genes and Genome (KEGG) pathways of phenylpropanoid biosynthesis, phenylalanine metabolism, plant hormone signal transduction and starch and sucrose metabolism. Genes related to microtubule, spindle, chromosomal kinetochore, vesicle, cellulose and processes of cytoplasm movement, chromatid segregation, membrane and cell wall development were inhibited by colchicine. Our results revealed that colchicine restrained the microtubules and inhibited gene expression of cytokinesis, which might slow down the cell activity, delay the cell into anaerobic respiration, resulting in apoptosis at late stage, and relieving of waterlogging.Kai ZhouPaige FleetEviatar NevoXinquan ZhangGenlou SunNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kai Zhou Paige Fleet Eviatar Nevo Xinquan Zhang Genlou Sun Transcriptome analysis reveals plant response to colchicine treatment during on chromosome doubling
description	Abstract Colchicine was commonly used to artificially double chromosomes while the transcriptome changes in colchicine treated plants has rarely been characterized. To understand the molecular mechanism of colchicine on chromosome doubling, we characterized transcriptome data of diploid orchardgrass root after colchicine treatment. Our results showed that 3381 of differentially expressed genes (DEGs) were mainly affected by water stress, 1258 DEGs that were expressed significantly in sample DacR5tr but not in DacR5ck were considered to be mainly affected by colchicine and combination of water and colchicine. These DEGs mainly regulated by colchicine were enriched to gene ontology (GO) accessions of cation binding, catalytic activity, membrane and transporter activity, and enriched to Kyoto Encyclopedia of Genes and Genome (KEGG) pathways of phenylpropanoid biosynthesis, phenylalanine metabolism, plant hormone signal transduction and starch and sucrose metabolism. Genes related to microtubule, spindle, chromosomal kinetochore, vesicle, cellulose and processes of cytoplasm movement, chromatid segregation, membrane and cell wall development were inhibited by colchicine. Our results revealed that colchicine restrained the microtubules and inhibited gene expression of cytokinesis, which might slow down the cell activity, delay the cell into anaerobic respiration, resulting in apoptosis at late stage, and relieving of waterlogging.
format	article
author	Kai Zhou Paige Fleet Eviatar Nevo Xinquan Zhang Genlou Sun
author_facet	Kai Zhou Paige Fleet Eviatar Nevo Xinquan Zhang Genlou Sun
author_sort	Kai Zhou
title	Transcriptome analysis reveals plant response to colchicine treatment during on chromosome doubling
title_short	Transcriptome analysis reveals plant response to colchicine treatment during on chromosome doubling
title_full	Transcriptome analysis reveals plant response to colchicine treatment during on chromosome doubling
title_fullStr	Transcriptome analysis reveals plant response to colchicine treatment during on chromosome doubling
title_full_unstemmed	Transcriptome analysis reveals plant response to colchicine treatment during on chromosome doubling
title_sort	transcriptome analysis reveals plant response to colchicine treatment during on chromosome doubling
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/24cff67896ef458396b36e8b8d22af8c
work_keys_str_mv	AT kaizhou transcriptomeanalysisrevealsplantresponsetocolchicinetreatmentduringonchromosomedoubling AT paigefleet transcriptomeanalysisrevealsplantresponsetocolchicinetreatmentduringonchromosomedoubling AT eviatarnevo transcriptomeanalysisrevealsplantresponsetocolchicinetreatmentduringonchromosomedoubling AT xinquanzhang transcriptomeanalysisrevealsplantresponsetocolchicinetreatmentduringonchromosomedoubling AT genlousun transcriptomeanalysisrevealsplantresponsetocolchicinetreatmentduringonchromosomedoubling
_version_	1718388942995193856

Transcriptome analysis reveals plant response to colchicine treatment during on chromosome doubling

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