Spectral fingerprinting to evaluate effects of storage conditions on biomolecular structure of filter-dried saliva samples and recovered DNA

Abstract Saliva has been widely recognized as a non-invasive, painless and easy-to-collect bodily fluid, which contains biomarkers that can be used for diagnosis of both oral and systemic diseases. Under ambient conditions, salivary biomarkers are subject to degradation. Therefore, in order to minim...

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Autores principales:	Raffaele Brogna, Harriëtte Oldenhof, Harald Sieme, Willem F. Wolkers
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/24d31be547c8401abb3f6c4fde07df31
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spelling	oai:doaj.org-article:24d31be547c8401abb3f6c4fde07df312021-12-02T12:33:15ZSpectral fingerprinting to evaluate effects of storage conditions on biomolecular structure of filter-dried saliva samples and recovered DNA10.1038/s41598-020-78306-12045-2322https://doaj.org/article/24d31be547c8401abb3f6c4fde07df312020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78306-1https://doaj.org/toc/2045-2322Abstract Saliva has been widely recognized as a non-invasive, painless and easy-to-collect bodily fluid, which contains biomarkers that can be used for diagnosis of both oral and systemic diseases. Under ambient conditions, salivary biomarkers are subject to degradation. Therefore, in order to minimize degradation during transport and storage, saliva specimens need to be stabilized. The aim of this study was to investigate the feasibility of preserving saliva samples by drying to provide a shelf-stable source of DNA. Human saliva was dried on filters under ambient conditions using sucrose as lyoprotective agent. Samples were stored under different conditions, i.e. varying relative humidity (RH) and temperature. In addition to assessment of different cell types in saliva and their DNA contents, Fourier transform infrared spectroscopy (FTIR) was used to evaluate the effects of storage on biomolecular structure characteristics of saliva. FTIR analysis showed that saliva dried without a lyoprotectant exhibits a higher content of extended β-sheet protein secondary structures compared to samples that were dried with sucrose. In order to evaluate differences in characteristic bands arising from the DNA backbone among differently stored samples, principal component analysis (PCA) was performed, allowing a clear discrimination between groups with/without sucrose as well as storage durations and conditions. Our results indicated that saliva dried on filters in the presence of sucrose exhibits higher biomolecular stability during storage.Raffaele BrognaHarriëtte OldenhofHarald SiemeWillem F. WolkersNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Raffaele Brogna Harriëtte Oldenhof Harald Sieme Willem F. Wolkers Spectral fingerprinting to evaluate effects of storage conditions on biomolecular structure of filter-dried saliva samples and recovered DNA
description	Abstract Saliva has been widely recognized as a non-invasive, painless and easy-to-collect bodily fluid, which contains biomarkers that can be used for diagnosis of both oral and systemic diseases. Under ambient conditions, salivary biomarkers are subject to degradation. Therefore, in order to minimize degradation during transport and storage, saliva specimens need to be stabilized. The aim of this study was to investigate the feasibility of preserving saliva samples by drying to provide a shelf-stable source of DNA. Human saliva was dried on filters under ambient conditions using sucrose as lyoprotective agent. Samples were stored under different conditions, i.e. varying relative humidity (RH) and temperature. In addition to assessment of different cell types in saliva and their DNA contents, Fourier transform infrared spectroscopy (FTIR) was used to evaluate the effects of storage on biomolecular structure characteristics of saliva. FTIR analysis showed that saliva dried without a lyoprotectant exhibits a higher content of extended β-sheet protein secondary structures compared to samples that were dried with sucrose. In order to evaluate differences in characteristic bands arising from the DNA backbone among differently stored samples, principal component analysis (PCA) was performed, allowing a clear discrimination between groups with/without sucrose as well as storage durations and conditions. Our results indicated that saliva dried on filters in the presence of sucrose exhibits higher biomolecular stability during storage.
format	article
author	Raffaele Brogna Harriëtte Oldenhof Harald Sieme Willem F. Wolkers
author_facet	Raffaele Brogna Harriëtte Oldenhof Harald Sieme Willem F. Wolkers
author_sort	Raffaele Brogna
title	Spectral fingerprinting to evaluate effects of storage conditions on biomolecular structure of filter-dried saliva samples and recovered DNA
title_short	Spectral fingerprinting to evaluate effects of storage conditions on biomolecular structure of filter-dried saliva samples and recovered DNA
title_full	Spectral fingerprinting to evaluate effects of storage conditions on biomolecular structure of filter-dried saliva samples and recovered DNA
title_fullStr	Spectral fingerprinting to evaluate effects of storage conditions on biomolecular structure of filter-dried saliva samples and recovered DNA
title_full_unstemmed	Spectral fingerprinting to evaluate effects of storage conditions on biomolecular structure of filter-dried saliva samples and recovered DNA
title_sort	spectral fingerprinting to evaluate effects of storage conditions on biomolecular structure of filter-dried saliva samples and recovered dna
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/24d31be547c8401abb3f6c4fde07df31
work_keys_str_mv	AT raffaelebrogna spectralfingerprintingtoevaluateeffectsofstorageconditionsonbiomolecularstructureoffilterdriedsalivasamplesandrecovereddna AT harrietteoldenhof spectralfingerprintingtoevaluateeffectsofstorageconditionsonbiomolecularstructureoffilterdriedsalivasamplesandrecovereddna AT haraldsieme spectralfingerprintingtoevaluateeffectsofstorageconditionsonbiomolecularstructureoffilterdriedsalivasamplesandrecovereddna AT willemfwolkers spectralfingerprintingtoevaluateeffectsofstorageconditionsonbiomolecularstructureoffilterdriedsalivasamplesandrecovereddna
_version_	1718393848121524224

Spectral fingerprinting to evaluate effects of storage conditions on biomolecular structure of filter-dried saliva samples and recovered DNA

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